AF-IM-13

Airfuge ®

Air-Driven Ultracentrifuge

Instruction Manual
Symbol Simbolo
Symbol
Title / Titel / Titre / Titulo / Titolo / /
Symbole
Símbolo

Dangerous voltage
Gefährliche elektrische Spannung
Courant haute tension
Voltaje peligroso
Pericolo: alta tensione

Attention, consult accompanying documents

Achtung! Begleitpapiere beachten!
Attention, consulter les documents joints
Atención, consulte los documentos adjuntos
! Attenzione: consultare le informazioni allegate

On (power)
Ein (Netzverbindung)
Marche (mise sous tension)
Encendido
Acceso (sotto tensione)

Off (power)
Aus (Netzverbindung)
Arrêt (mise hors tension)
Apagado
Spento (fuori tensione)

Protective earth (ground)

Schutzleiteranschluß
Liaison à la terre
Puesta a tierra de protección
Collegamento di protezione a terra

Earth (ground)
Erde
Terre
Tierra
Scarica a terra
 ! SAFETY NOTICE
This safety notice summarizes information basic to the safe operation of the equipment
described in this manual. The international symbol displayed above is a reminder that all
safety instructions should be read and understood before installation, operation, mainte-
nance, or repair of this instrument. When you see the symbol on other pages, pay special
attention to the safety information presented. Observance of safety precautions will also
help to avoid actions that could damage or adversely affect the performance of the
equipment.

Safety During Installation and/or Maintenance

Locate the ultracentrifuge in a clean, safe, uncluttered environment.

Any servicing of this equipment that requires removal of any covers can expose parts
which involve the risk of electric shock or personal injury. Make sure that the power
switch is off and the ultracentrifuge is disconnected from the main power source, and refer
such servicing to qualified personnel.

Do not replace any ultracentrifuge components with parts not specified for use on this
instrument.

Electrical Safety

To reduce the risk of electrical shock, this instrument uses a three-wire electrical cord and
plug to connect this equipment to earth-ground. To preserve this safety feature:

• Make sure that the matching wall outlet receptacle is properly wired and earth-
grounded. Check that the line voltage agrees with the voltage listed on the name-rating
plate affixed to the instrument.

• Never use a three-to-two wire plug adapter.

• Never use a two-wire extension cord or a two-wire non-grounding type of multiple-
outlet receptacle strip.

Do not place containers holding liquid on or near the chamber door. If they spill, liquid
may get into the instrument and damage electrical or mechanical components.

Safety Against Risk of Fire

Fuses protect certain electrical circuits within this instrument against overcurrent condi-
tions. For continued protection against the risk of fire, replace only with the same type and
rating specified.

This instrument is not designed for use with materials capable of developing flammable or
explosive vapors. Do not centrifuge such materials (such as chloroform or ethyl alcohol)
in this instrument nor handle or store them within the required 30-cm (1-ft) area
surrounding the ultracentrifuge.
Mechanical Safety

For safe operation of the equipment, observe the following:

• Use only the rotors and accessories designed for use in this ultracentrifuge.

• Never operate the instrument without a rotor cap or lid installed on the rotor.

• Do not exceed the maximum rated speed of the rotor in use.

• NEVER attempt to slow or stop the rotor by hand.

• Do not lift or move the ultracentrifuge while the rotor is spinning.

• NEVER open the chamber door while the rotor is spinning. The instrument has a
protective window to allow safe observation of rotor motion.

• Maintain a 7.6-cm (3-in.) clearance envelope around the ultracentrifuge while it is

running. During operation you should come within the envelope only to adjust instru-
ment controls, if necessary. Never bring any flammable substances within the 30-cm
(1-ft) area surrounding the ultracentrifuge. Never lean on the instrument or place items
on the ultracentrifuge while it is operating.

Chemical and Biological Safety

Normal operation may involve the use of solutions and test samples that are pathogenic,
toxic, or radioactive. Such materials should not be used in this ultracentrifuge, however,
unless all necessary safety precautions are taken.

• Observe all cautionary information printed on the original solution containers prior to
their use.

• Handle body fluids with care because they can transmit disease. No known test offers
complete assurance that they are free of micro-organisms. Some of the most virulent—
Hepatitis (B and C) and HIV (I–V) viruses, atypical mycobacteria, and certain systemic
fungi—further emphasize the need for aerosol protection. Handle other infectious
samples according to good laboratory procedures and methods to prevent spread of
disease. Because spills may generate aerosols, observe proper safety precautions for
aerosol containment. Do not run toxic, pathogenic, or radioactive materials in this
instrument without taking appropriate safety precautions. Biosafe containment should
be used when Risk Group II materials (as identified in the World Health Organization
Laboratory Biosafety Manual) are handled; materials of a higher group require more
than one level of protection.

• Dispose of all waste solutions according to appropriate environmental health and safety
guidelines.

It is your responsibility to decontaminate the instrument and accessories before

requesting service by a Beckman Coulter representative.
 AF-IM-13
December 2007

Airfuge®
Air-Driven Ultracentrifuge

Instruction Manual

© 2007 Beckman Coulter, Inc.

 Contents

Page

INTRODUCTION
Certification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . vii
Scope of Manual. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . vii
Conventions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . viii
Notes, Cautions, and Warnings . . . . . . . . . . . . . . . . . . . . . . . . . . . viii
CFC-Free Centrifugation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix
Radio Interference . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix
Canadian Regulations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . x
Recycling Label . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . x

SECTION 1: DESCRIPTION
Functional Description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-1
Safety Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-2
Controls and Indicators . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-2
Pressure Regulator Knob . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-2
Pressure Gauge . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-2
Run Light . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-2
Time-Delay Knob . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-2
Brake Tension Screw. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-3
Levitation Air Screw . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-3
Chamber Components . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-5
Stator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-5
Stator Pad. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-5
Brake Pin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-6
Door Latch . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-6
Filter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-6
Name Rating Plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-6
Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-7
Available Rotors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-8

iii
Contents

Page

SECTION 2: INSTALLATION
Space and Location Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-1
Electrical Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-2
Installation Procedures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-2
Filter Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-3
Time-Delay Setting Check . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-4
Leveling the Airfuge . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-4
Installing the Brake Pin and Stator Pad . . . . . . . . . . . . . . . . . . . . . 2-5
Trial Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-6
Cold Room Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-6

SECTION 3: OPERATION
Starting the Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-2
Ending the Run. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-4

SECTION 4: TROUBLESHOOTING

SECTION 5: MAINTENANCE
Instrument Care . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-1
General Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-2
Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-2
Instrument Interior and Exterior . . . . . . . . . . . . . . . . . . . . . . . . . . 5-3
Stator Assembly. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-3
Lubrication . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-4
Filter Element Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-4
Brake Spring Wire Adjustment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-6
Brake Spring Wire Tension Adjustment . . . . . . . . . . . . . . . . . . . . . . . 5-7
Levitation Air Adjustment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-8
Time-Delay Period Adjustment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-9
Decontamination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-10
Sterilization and Disinfection. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-10
Storage and Transportation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-11
Supply List . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-11
Supplies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-11

Warranty

iv
 Illustrations and Tables

Page

Figure 1-1. The Airfuge Ultracentrifuge. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-1

Figure 1-2. Bottom Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-3
Figure 1-3. Cross-Section View of the Airfuge . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-4
Figure 1-4. Rear View. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-4
Figure 1-5. Airfuge Components and Accessories . . . . . . . . . . . . . . . . . . . . . . . . . 1-5

Figure 2-1. Filter Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-3

Figure 2-2. Aligning the Brake Pin in the Stator . . . . . . . . . . . . . . . . . . . . . . . . . . 2-5
Figure 2-3. Cold Room Set-up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-7

Figure 3-1. Worn and New Stator Pads . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-3

Figure 5-1. Prying Up the Stator. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-5

Figure 5-2. Lifting Out the Stator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-5
Figure 5-3. Stator O-Ring Positions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-5
Figure 5-4. Reassembling the Stator and Pin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-5
Figure 5-5. Location of the Brake Spring Wire from the Ultracentrifuge
Bottom . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-7
Figure 5-6. Location of Rear Controls . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-8
Figure 5-7. Location of Time-Delay Knob . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-9

Table 4-1. Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-2

v
 Introduction

CERTIFICATION
To ensure full system quality, Beckman Coulter Airfuge® ultracentri-
fuges are manufactured in a registered ISO 9001 or 13485 facility.
They have been designed and tested to be compliant (when used with
Beckman Coulter rotors) with the laboratory equipment requirements
of applicable regulatory agencies. Declarations of conformity and
certificates of compliance are available at www.beckmancoulter.com.

SCOPE OF MANUAL
This manual is designed to familiarize you with the Airfuge ultracen-
trifuge, its functions, specifications, operation, and routine operator
care and maintenance. We recommend that you read this entire
manual, especially the SAFETY NOTICE and all safety-related infor-
mation, before operating the ultracentrifuge or performing instrument
maintenance.

• Section 1 contains system specifications and a brief physical and

functional description of the ultracentrifuge, including the
operating controls and indicators.

• Section 2 provides information about space and power require-

ments for installing and connecting the ultracentrifuge.

• Section 3 contains ultracentrifuge operating procedures.

• Section 4 lists possible malfunctions, together with probable

causes and suggested corrective actions.

• Section 5 contains procedures for routine operator care and main-

tenance, as well as a brief list of supplies and replacement parts.

vii
Introduction

➠ NOTE
If the ultracentrifuge is used in a manner other
than specified in this manual, the safety and
performance of this equipment could be
impaired. Further, the use of any equipment
other than that recommended by Beckman
Coulter has not been evaluated for safety. Use of
any equipment not specifically recommended in
this manual and/or the appropriate rotor manual
is the sole responsibility of the user.

CONVENTIONS
Certain symbols are used in this manual to call out safety-related and
other important information. These international symbols may also be
displayed on the ultracentrifuge and are reproduced and described
below and on the inside of the front cover.

NOTES, CAUTIONS, AND WARNINGS

➠ NOTE
Used to call attention to important information
that should be followed during installation, use,
or servicing of this equipment.

! CAUTION
Used to indicate a potentially hazardous situa-
tion which, if not avoided, may result in minor or
moderate injury and/or mechanical damage. It
is also used to alert against unsafe practices.

viii
 Introduction

! WARNING
Used whenever an action or condition may
potentially cause personal injury or loss of
life. Mechanical damage may also result.

WARNING
Indicates high voltage or risk of electric
shock. Refer servicing of all areas displaying
either symbol to service personnel.

CFC-FREE CENTRIFUGATION
To ensure minimal environmental impact, no CFCs are used in the
manufacture or operation of Airfuge ultracentrifuges.
CFC

RADIO INTERFERENCE
This equipment has been tested and found to comply with the limits
for a Class A digital device, pursuant to Part 15 of FCC Rules. These
limits are designed to provide reasonable protection against harmful
interference when the equipment is operated in a commercial environ-
ment. This equipment generates, uses, and can radiate radio
frequency energy and, if not installed and used in accordance with
this instruction manual, may cause interference to radio communica-
tions. Operation of this equipment in a residential area is likely to
cause interference, in which case the user will be required to correct
the interference at his own expense.

ix
Introduction

CANADIAN REGULATIONS

This equipment does not exceed the Class A limits for radio noise
emissions from digital apparatus as set out in the radio interference
regulations of the Canadian Department of Communications.

Le présent appareil numérique n’émet pas de bruits radioélectriques

dépassant les limites applicables aux appareils numériques de Classe
A prescrites dans le reglement sur le brouillage radioelectrique édicté
par le Ministère des Communications du Canada.

RECYCLING LABEL
This symbol is required in accordance with the Waste Electrical and
Electronic Equipment (WEEE) Directive of the European Union. The
presence of this marking on the product indicates:

A28219-AA
1) the device was put on the European market after August 13, 2005
and
Note: On the instrument, the
triangle background is yellow 2) the device is not to be disposed via the municipal waste collection
rather than gray. system of any member state of the European Union.

It is very important that customers understand and follow all laws

regarding the proper decontamination and safe disposal of electrical
equipment. For Beckman Coulter products bearing this label please
contact your dealer or local Beckman Coulter office for details on the
take back program that will facilitate the proper collection, treatment,
recovery, recycling and safe disposal of the device.

x
 1
Description

FUNCTIONAL DESCRIPTION
The Beckman Coulter Airfuge® (see Figure 1-1) is a benchtop
air-driven ultracentrifuge capable of accelerating rotors up to
110 000 rpm (revolutions per minute) in as little as 30 seconds. The
instrument uses no vacuum or high-speed bearings; the rotor is
supported and turned by streams of air. Rotor speed can be deter-
mined by conversion of the applied air pressure, as displayed on the
PRESSURE gauge, to rpm.1

An available Digital Speed Readout Accessory (347592), which

attaches to the top of the chamber door, continuously displays actual
rotor speed. Installation and use of the accessory are described in
publication AF-TB-013.

Pressure Regulator Knob

Run Light

Figure 1-1. The Airfuge Ultracentrifuge

1 This conversion is different for each type of rotor (see the graph in the applicable rotor manual).

1-1
Description

SAFETY FEATURES
The Airfuge ultracentrifuge has been designed and tested to operate
safely indoors at altitudes up to 2 000 m (6 562 ft).

The Airfuge is an exceptionally safe ultracentrifuge because the rotor

is held in place by a pressure differential created during centrifugation.
An air pressure limitation of 35 psig (240 kPa) is factory set. However,
do not operate the instrument at more than 30 psig (207 kPa).

CONTROLS AND INDICATORS

PRESSURE REGULATOR KNOB

The pressure regulator knob controls the driving air pressure. Pressing
down on the knob and turning to the right (clockwise) closes the door.
Turning the knob further brings up the air pressure and accelerates the
rotor to speed.

PRESSURE GAUGE

Operating air pressure is displayed on this gauge. Recommended

operating air pressure is 30 psig (207 kPa).

RUN LIGHT

The run light comes on when the timer is set and remains lit until the
braking system is engaged.

TIME-DELAY KNOB

This knob, located on the ultracentrifuge bottom panel (see Figure 1-2),
is used to set a time-delay period that allows the rotor to coast after
the timer reaches zero at the end of a run. The run light goes out and
a brake pin is released at the end of the set delay period. A setting of
120 seconds on the knob (or about 3.5 on older instruments) represents
the 2-minute delay period.

1-2
 Description

Time Delay Knob

Figure 1-2. Bottom Panel

BRAKE TENSION SCREW

The brake spring wire (see Figure 1-3) forces the brake pin up into
the rotor bottom during braking. Occasional adjustment of the spring
tension, using the BRAKE TENSION adjusting screw located on the
back panel (see Figure 1-4), may be necessary.

LEVITATION AIR SCREW

When the timer reaches zero at the end of a run, the driving air is
turned off and levitation air supports the rotor during its deceleration.
If the flow of levitation air is too high and the rotor fails to stop
completely, or too low and the rotor stops too fast, adjustment of the
LEVITATION AIR screw located on the rear panel may be necessary.

1-3
Description

Instrument
Door Protective Window

Stator Turbine
Pad Flutes
Stator

Driving
Levitation
Air Passage
Air Passage

Brake Brake Pin

Spring Wire
Electromagnet

Figure 1-3. Cross-section View of the Airfuge

Figure 1-4. Rear View

1-4
 Description

CHAMBER COMPONENTS
STATOR

The brass stator (see Figure 1-3) contains the driving air jets
that control the rotor speed and the air jets that levitate the rotor
during deceleration. Levitation air operates automatically during
deceleration.

STATOR PAD

The black stator pad (see Figure 1-5) rests on the channel of the stator
and cushions the rotor during a run and when the rotor is at rest.

Figure 1-5. Airfuge Components and Accessories

1-5
Description

BRAKE PIN

Braking action is provided by a movable brake pin (see Figure 1-5)

set vertically on a spring in the shaft of the stator (see Figure 1-3).
An electromagnet holds the pin down during a run and releases it
2 minutes after the timer reaches zero. When released, the pin
engages the rotor bottom, stabilizing the rotor as it gradually
decelerates to a stop.

DOOR LATCH

The door-latch mechanism secures the door during centrifugation.

This prevents premature door opening, which would result in damage
to the rotor and stator pad.

FILTER
A filter is supplied with the ultracentrifuge for water and oil removal
from the air supply. The filter element turns red when it is saturated
and needs to be replaced.

NAME RATING PLATE

A name rating plate is affixed to the rear of the instrument. Always
mention the serial number and model number when contacting
Beckman Coulter regarding your Airfuge ultracentrifuge.

1-6
 Description

SPECIFICATIONS
Only values with tolerances or limits are guaranteed data. Values
without tolerances are informative data, without guarantee.

Speed range . . . . . . . . . . . . . . . . . . . . . . . . 80 000 to 110 000 (±5 000) rpm

Maximum relative centrifugal field*. . . . . . . . . . . . . . . . . . . . . 199 000 × g
Air pressure:
Air pressure at ultracentrifuge for
routine operation . . . . . . . . . . . . . . . . . . . . . . . . . 30 psig (207 kPa)
Air pressure at filter for routine operation . . . . . . . . 42 psig (290 kPa)
Air pressure drop across filter . . . . . . . . . . . . . . . . . . 12 psig (83 kPa)
Maximum air pressure at ultracentrifuge . . . . . . . . . 75 psig (517 kPa)
Minimum air pressure at ultracentrifuge
for operation. . . . . . . . . . . . . . . . . . . . . . . . . . . . 20 psig (138 kPa)†
Maximum air flow rate at 30 psig (207 kPa) . . . . . . 0.0015 m3/s (3.5 cfm)
Time. . . . . . . . . . . . . . . . . . . . . . . . . . . . to 5 hours in 5-minute increments
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . or continuous (hold)
Approximate acceleration time . . . . . . . . . . . . . . . . . . . . 30 to 60 seconds
Approximate deceleration time . . . . . . . . . . . . . . . . . 2 1/2 to 5 1/2 minutes
Rotor temperature . . . . . . . rotor temperature is always a few degrees above
. . . . . . . . . . . . . . . . . . ambient temperature; the instrument can be run
. . . . . . . . . . . . . . . . . . . . . . . . . . in a cold room for lower temperatures
Dimensions
Width . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28 cm (11 in.)
Depth . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38 cm (15 in.)
Height. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20 cm (8 in.)
Weight . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10.4 kg (23 lb)
Finishes
Control panel . . . . . . . . . . . . . . . . . . . . . . . . . . . coated polycarbonate
Top surface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . urethane paint
Other surfaces . . . . . . . . . . . . . . . . . . . . . . . . . . general purpose paint
Electrical requirements
60-Hz instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 120 VAC
50-Hz instrument . . . . . . . . . . . . . . . . . . . . . . . . 100 or 220–240 VAC
Electrical supply . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Class I
Installation (overvoltage) category . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . II
Pollution degree. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2‡

* Relative Centrifugal Field (RCF) is the ratio of the centrifugal acceleration at a specified radius
and speed (rω 2) to the standard acceleration of gravity (g) according to the following formula:

rω 2
RCF = --------
-
g

where r is the radius in millimeters, ω is the angular velocity in radians per second
(2 π RPM /60), and g is the standard acceleration of gravity (9807 mm/s2). After substitution:

RPM 2
RCF = 1.12 r ⎛⎝ ------------⎞⎠
1000

† Instrument operation at gauge pressures below 20 psig (138 kPa) may cause rotor instability.
‡ Normally only nonconductive pollution occurs; occasionally, however, a temporary conduc-
tivity caused by condensation must be expected.

1-7
Description

AVAILABLE ROTORS
Airfuge rotors are made of anodized aluminum and have turbine
flutes on the bottom that provide the driving surface for the jets of air.
Pressurized air impinges on the rotor bottom and lifts and turns the
rotor. A white plastic bushing fitted in the rotor bottom is engaged by
the brake pin during braking.

Rotors are designed to meet specific needs. Detailed information

about each rotor is contained in the applicable rotor manual.

• The A-110 fixed angle rotor holds up to six 180-µL tubes at a tube
angle of 18 degrees from the axis of rotation, and is ideal for the
rapid separation of small sample volumes in a very short time.
Maximum operating speed is 110 000 rpm at 30 psig (207 kPa) air
pressure.

• The A-100/18 fixed angle rotor holds up to six 175-µL tubes at

a tube angle of 18 degrees from the axis of rotation, and is ideal
for the efficient sedimentation of small sample volumes in a very
short time. Maximum operating speed is 95 000 rpm at 30 psig
(207 kPa) air pressure.

• The A-100/30 fixed angle rotor holds up to six 240-µL tubes at

a tube angle of 30 degrees from the axis of rotation, and is ideal
for the efficient sedimentation of small sample volumes in a very
short time. Maximum operating speed is 92 000 rpm at 30 psig
(207 kPa) air pressure.

• The A-95 fixed angle rotor holds up to four 450-μL tubes at a tube
angle of 30 degrees from the axis of rotation, and is ideal for the
rapid separation of small sample volumes in a very short time or
to isolate microsomal fractions and plasma membranes. It can also
be used to clarify small volumes of lipemic serum by flotation of
the chylomicrons. Maximum operating speed is 95 000 rpm at
30 psig (207 kPa) air pressure.

• The ACR-90 rotor uses 3.5-mL and 2.4-mL disposable liners,

and is used to clarify lipemic serum. Serum can be clarified by a
10-minute spin at 90 000 rpm at 30 psig (207 kPa) air pressure.

• The EM-90 electron microscopy particle-counting rotor is specifi-

cally designed for sedimenting particulates from very small
volumes onto supports suitable for insertion into an electron
microscope. The rotor is rated for 90 000 rpm at 30 psig (207 kPa)
air pressure.

• The Batch rotor can be used to pellet particles from a single large
sample. Sample volumes of 2 to 7 mL can be run. The rotor is
rated for 90 000 rpm at 30 psig (207 kPa) air pressure.

1-8
 2
Installation

SPACE AND LOCATION REQUIREMENTS

! WARNING
Do not place the ultracentrifuge near areas
containing flammable reagents or combus-
tible fluids. Vapors from these materials
could enter the ultracentrifuge air system and
be ignited by the motor. Maintain a 30-cm
(1-ft) clearance envelope around the ultracen-
trifuge while it is running. No persons or any
hazardous materials should be within this
clearance boundary while the ultracentrifuge
is operating except to change operating
controls, if required.

• Select a location away from heat-producing laboratory equipment,

with sufficient ventilation to allow for heat dissipation.

• Position the ultracentrifuge on a level surface, such as a sturdy

table or laboratory bench, within 4 m (12 ft) of a supply of clean,
compressed air at about 42 psig (290 kPa), but not over 75 psig
(517 kPa).

• In addition to space for the ultracentrifuge itself, allow 7.6-cm

(3-in.) clearances at the sides and back of the ultracentrifuge to
ensure sufficient air circulation.

2-1
Preinstallation Requirements

ELECTRICAL REQUIREMENTS
60-Hz instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 120 VAC
50-Hz instrument . . . . . . . . . . . . . . . . . . . . . . . 100 or 200–240 VAC

To reduce the risk of electrical shock, this ultracentrifuge uses a three-

wire electrical cord and plug to connect the ultracentrifuge to earth-
ground. (Contact your local Beckman Coulter office for specific
information regarding local requirements.) To preserve this safety
feature:

• Make sure that the matching wall outlet receptacle is properly

wired and earth-grounded. Check that the line voltage agrees with
the voltage listed on the name rating plate affixed to the ultracen-
trifuge. Then plug in both ends of the ultracentrifuge power cord.

• Never use a three-to-two wire plug adapter.

• Never use a two-wire extension cord or a two-wire non-grounding

type of multiple-outlet receptacle strip.

• If there is any question about voltage, have a qualified service

person measure it under load while the drive is operating.

To ensure safety the ultracentrifuge should be wired to a remote emer-

gency switch (preferably outside the room where the ultracentrifuge
is housed, or adjacent to the exit from that room), in order to
disconnect the ultracentrifuge from the main power source in case
of a malfunction.

INSTALLATION PROCEDURES
Installation of the ultracentrifuge requires the following steps—in
order:

• installing the filter,

• checking the time-delay setting,

• leveling the instrument, and

• installing the brake pin and stator pad.

After installation, perform a trial run to ensure proper operation.

2-2
 Preinstallation Requirements

FILTER INSTALLATION

➠ NOTE
If the air source is especially wet or oily, install a
second filter as a prefilter. A second filter will
cause an additional pressure drop of approxi-
mately 5 psig (35 kPa) in the air pressure that
reaches the instrument.

Make sure the required operating air pressure is obtainable at the

instrument PRESSURE gauge.

1. Pull back the spring-loaded sleeve of the quick-disconnect at the

filter outlet and push the fitting of the quick-disconnect hose into
the sleeve until they snap together (see Figure 2-1).

➠ NOTE
Do not install the filter upside down. The filter
bottom has a valve that distinguishes it from the
top. Escaping air can be heard if the filter is
Filter Bottom installed upside down.

From Air Source From Air

Source
Quick-disconnect
hose from filter Quick-disconnect
to Airfuge hose from filter
Ultracentrifuge to Airfuge
Barb
Fitting Ultracentrifuge

Arrow indicates
direction of airflow Arrow indicates
Remove barb fitting
direction of airflow
before installing 1/4"
piping directly into filter

Mounting
Screws (4)

Filter Mounted On The Wall Free Standing Filter

Figure 2-1. Filter Installation

2-3
Preinstallation Requirements

2. If non-rigid piping leads from the air source, mount the filter on a
ring stand or on the wall. Clamp the filter to a ring stand, or use the
supplied mounting brackets (see Figure 2-1) to mount the filter
to the wall. Attach the brackets to the assembled filter with the
mounting screws, then attach the brackets to the wall. The air flow
arrow must point toward the ultracentrifuge. Add hose clamps to
the plain high-pressure hose. Clamp one end of the hose to the
barb fitting of the filter inlet and one end to the air source.

If rigid piping (standard 1/4-in. pipe thread) leads from the air
source, the filter can be free-standing. Remove the barb fitting
from the filter inlet to the air source. The air flow arrow must point
toward the ultracentrifuge.

3. Connect the quick-disconnect hose to the fitting at the rear of the

instrument. Place a beaker or other container under the filter to
collect water and oil.

If the air source does not supply sufficient air pressure to close the
filter’s automatic drain valve you will hear air escaping from the
filter. Routine operation at 30 psig (207 kPa) on the instrument
PRESSURE gauge requires 290 kPa (42 psig) air pressure supplied to
the filter.

TIME-DELAY SETTING CHECK

The time-delay knob (on the ultracentrifuge bottom plate) is factory

set for a 2-minute delay period. Make sure that the knob is still set at
about 120 seconds (or 3.5 on older instruments).

LEVELING THE AIRFUGE

The Airfuge must be leveled before use.

1. Place the supplied liquid level on the center of the stator.

2. Adjust the instrument front feet until the bubble of the level is
centered.

2-4
 Preinstallation Requirements

INSTALLING THE BRAKE PIN AND STATOR PAD

1. Place the brake pin in the hole at the center of the stator (see
Figure 2-2). Make sure that the slot in the pin bottom straddles
the brake spring wire as shown in the figure.

2. Lightly touch the pin. It should bounce back freely to about 3 mm

(1/8 in.) above the stator. If the pin is too high (approximately
6 mm above the stator), gently turn the pin so that it drops down
to straddle the spring wire.

3. Install the black ring-shaped stator pad in the channel of the stator.

Brake Pin

Stator Screw Stator Screw

Stator Pad

Stator Brake Pin Slot

Brake
Spring Wire

WRONG

Figure 2-2. Aligning the Brake Pin in the Stator. The slot on the end of the pin straddles the brake spring wire.
The broken line shows the wire direction (aligned with the screws). If the tip of the pin slot stands
on the wire instead of straddling it, the pin will be too high above the stator.

2-5
Preinstallation Requirements

TRIAL RUN

When installation is complete, perform a trial run to confirm proper

ultracentrifuge operation. Refer to Section 3 for operating instruc-
tions. Time the rotor deceleration as follows.

1. After the rotor runs for a few minutes, turn the TIME dial to zero
and begin timing. The run light should go out approximately
2 minutes after the TIME dial is turned off.

2. Observe rotor motion through the window. Fixed angle rotors

should stop within about 2 minutes after the run light goes out.
Other rotors should stop within about 3-1/2 minutes.

➠ NOTE
If a fixed angle rotor stops in less than
45 seconds, sample stirring may result; adjust
the brake or levitation air to provide a longer
stopping time. If a rotor takes too long to stop,
the brake, pin tension, or levitation air needs
adjusting. See Section 5 for adjustment
procedures.

COLD ROOM INSTALLATION

If rotor temperatures below room temperature are required, the

Airfuge can be operated in a cold room. A typical cold room set-up is
shown in Figure 2-3. In addition to the installation procedures above,
the following variations are required.

• Because large amounts of water may condense in the airstream

as it passes through the lower temperatures of the cold room, a
second filter is required to remove the additional water. Install
the second filter as close as possible to the ultracentrifuge. The
maximum distance between the filter and the instrument should
be 0.6 m (2 ft).

• The second filter will cause a pressure drop of about 5 psig

(35 kPa) in the air pressure that reaches the instrument. Make
sure that the required operating air pressure is obtainable at the
instrument pressure gauge.

2-6
 Preinstallation Requirements

• Approximately 3 m (10 ft) of finned copper tubing (3/8-in. ID

recommended) must be added to the air supply line (see
Figure 2-3) to allow the air to cool to cold-room temperature.

• The operating temperature of the instrument will be a few degrees

higher than the cold-room temperature; the rotor will typically be
operating at approximately 8 to 10°C.

Compressor or
Pressure
Laboratory Air Supply
Gauge
Compressor Capacity
3.5 to 4 cfm at 47
psig (330 kPa)

Oil Removal
Filter
Regulator
(Norgren Part
No. 339620)

COLD ROOM
Water Removal Filter
(Norgren F11-200A3T)

0.6 m
(2 ft)
or less

3 m (10 ft) of
3/8 in. ID finned Airfuge Ultracentrifuge
copper tubing

Figure 2-3. Cold Room Set-up

2-7
 3
Operation

Refer to individual rotor manuals for detailed information on

each rotor.

! WARNING
Normal operation may involve the use of solu-
tions and test samples that are pathogenic,
toxic, or radioactive. Handle body fluids
with care because they can transmit disease.
No known test offers complete assurance that
they are free of micro-organisms. Some of the
most virulent—Hepatitis (B and C) and HIV
(I–V) viruses, atypical mycobacteria, and
certain systemic fungi—further emphasize
the need for aerosol protection. Handle other
infectious samples according to good labora-
tory procedures and methods to prevent
spread of disease. Because spills may generate
aerosols, observe proper safety precautions
for aerosol containment. Do not run toxic,
pathogenic, or radioactive materials in this
ultracentrifuge without taking appropriate
safety precautions. Biosafe containment
should be used when Risk Group II materials
(as identified in the World Health Organiza-
tion Laboratory Biosafety Manual) are
handled; materials of a higher group require
more than one level of protection.

3-1
Operation

! WARNING
Do not use the ultracentrifuge in the vicinity
of flammable liquids or vapors, and do not
run such materials in the instrument. Do not
lean on the instrument or place items on it
while it is operating.

STARTING THE RUN

1. Connect the power cord and turn the air supply on.

! CAUTION
Never operate the ultracentrifuge without a
rotor cap or lid installed on the rotor.

2. Load the rotor and secure the rotor lid or cap as described in the
applicable rotor manual. Tubes should be symmetrically loaded in
a fixed angle rotor; rotor liners should be full and evenly seated in
their rotor base.

➠ NOTE
If you are using an Airfuge with the digital
speed readout accessory installed, use a flat
black marker to color half of the rotor lid or cap
flat black. If the black surface is shiny, inaccu-
rate speed readings will be displayed.

3. Lightly touch the brake pin to be sure it operates freely.

4. Check the stator pad; it must be clean and in good condition.

A worn stator pad is smooth and shiny on the inside surface
(see Figure 3-1). Replace a worn stator pad.

➠ NOTE
Stator pads—especially the white circles on the
bottom—should be kept clean with alcohol.

3-2
 Operation

Worn
Stator Pads

New
Stator Pad

Figure 3-1. Worn and New Stator Pads

5. Place the rotor on the stator pad and shut the instrument door.

6. Set the TIME dial for the required run time. The dial indicates up
to 5 hours of run time in 5-minute increments. (In some models,
the dial indicates 5 minutes of run time in 5-second increments.)
For longer runs, set the timer to HOLD. For runs shorter than
30 minutes, turn the dial past the 30-minute point, then back to the
required time. (ACR-90 rotor—set the run time for 10 minutes to
prevent significant evaporative loss.)

7. Secure the instrument door by turning the pressure regulator knob

to the right (clockwise), pushing down, until the air pressure indi-
cated on the PRESSURE gauge brings the rotor to the required
speed. Optimal run speed is achieved at 30 psig (207 kPa). (This is
the only recommended operating air pressure for the ACR-90
rotor.) Note that some low-speed rotor vibration is normal at the
beginning of the run.

3-3
Operation

! CAUTION
If the rotor makes an unusual noise, turn the
timer to zero. When the rotor stops, open the
chamber door and remove the rotor. Check to
make sure the rotor is loaded correctly. Correct
the problem before restarting the run (see
Table 4-1).

ENDING THE RUN

1. When the timer reaches or is turned to zero, the rotor will coast for
about 2 minutes. After the 2-minute delay period, the run light
goes out and the brake pin engages the rotor. The rotor will stop
about 1 to 3 minutes later, depending on the rotor.

➠ NOTE
If the coasting time is too short—45 seconds or
less—check the time-delay relay setting and/or
adjust the flow of levitation air as described in
Section 5.

2. After the rotor has stopped, turn the pressure regulator knob to
the left (counterclockwise) until the PRESSURE gauge reading
is zero.

3. Open the chamber door. Refer to the applicable rotor manual for
instructions on unloading and cleaning the rotor.

➠ NOTE
Do not tighten the pressure regulator knob
between runs.

3-4
 4
Troubleshooting

This section lists possible malfunctions, together with probable

causes and corrective actions. Maintenance procedures are in
Section 5. For any problems not covered here, contact Beckman
Coulter Field Service for assistance.

➠ NOTE
It is your responsibility to decontaminate the
instrument, as well as any rotors and/or accesso-
ries, before requesting service by Beckman
Coulter Field Service representatives.

Possible malfunctions are described in Table 4-1, along with possible

causes—listed in the probable order of occurrence—and corrective
actions. Perform the recommended corrective action in sequence, as
listed. If you are unable to correct the problem, call Beckman Coulter
Field Service. To help diagnose and correct the problem, provide as
much information as possible:

• Note the operating situation when the error occurred (rotor in use,
speed, load type, and so forth).

• Note any unusual environmental and/or operating conditions

(ambient temperature, voltage fluctuations, and so forth).

• Include any other useful information.

4-1
Troubleshooting

Table 4-1. Troubleshooting. If the problem persists call Beckman Coulter Field Service.

Problem Possible Cause Recommended Action

Brake pin falls flat on stator Brake spring wire not centered Adjust the brake spring wire.
surface
Brake spring tension too low Adjust the brake spring tension.

Brake pin not actuating Brake spring tension too low Adjust the brake spring tension.

Stator shaft oily Clean the stator shaft and pin.

Brake pin damaged Replace the brake pin.

Rotor will not spin Air supply or power disconnected Check air and power connections.

Circuit breaker tripped Reset the circuit breaker.

Filter saturated Replace the filter element.

Stator pad worn or damaged Replace the stator pad.

Stator screws loose Check stator screws; tighten as required.

Rotor runs erratically or Stator pad damaged Replace the stator pad.
makes unusual noise
Dirty air jets Clean the stator assembly.

Missing or damaged rotor cap Check the rotor cap.

Rotor improperly loaded Check placement of tubes or liner in rotor.

Rotor fails to stop Brake pin shaft oily Clean the pin and shaft.

Levitation air flow too high Adjust levitation air.

Brake spring tension too low Adjust the brake spring tension.

Rotor stops too fast Levitation air flow too low Adjust levitation air.

Time-delay period too short Check the time-delay setting.

Stator pad worn or damaged Replace the stator pad.

Brake pin shaft oily Clean the stator assembly and shaft.

Brake malfunction Adjust brake spring tension and levitation air.

Oil visibly collecting in Filter element saturated Replace the filter element.
instrument

Filter element turns red Filter element saturated Replace the filter element.

Pressure at instrument will Filter element saturated Replace the filter element.
no longer read 30 psig

Continued —

4-2
 Troubleshooting

Table 4-1. Troubleshooting (continued)

Problem Possible Cause Recommended Action

Run light fails to come on Light burned out Replace the lamp.

Circuit breaker tripped Reset the circuit breaker.

Time delay faulty Call Beckman Coulter Field Service.

Rotor crashes Stator pad worn Order Repair Kit 1.

Missing rotor lid or cap Refer to the applicable rotor manual.

Circuit breaker trips Various Call Beckman Coulter Field Service.

repeatedly

! CAUTION
Repeated tripping of the circuit breaker may cause
instrument damage.

4-3
 5
Maintenance

This section contains care and maintenance procedures that should

be performed regularly. For maintenance not covered in this manual,
contact Beckman Coulter Field Service for assistance. Refer to the
applicable rotor manual for instructions on the care of rotors and
their accessories.

INSTRUMENT CARE

➠ NOTE
It is your responsibility to decontaminate the
instrument, as well as any rotors and/or accesso-
ries, before requesting service by Beckman
Coulter Field Service representatives.

WARNING
Any maintenance procedure requiring
removal of a panel exposes the operator to
the possibility of electrical shock and/or
mechanical injury. Therefore, turn the power
OFF and disconnect the instrument from the
main power source, and refer such mainte-
nance to service personnel.

5-1
Maintenance

GENERAL MAINTENANCE
Perform the following procedures regularly to ensure satisfactory
performance and long service life of the ultracentrifuge.

• At least once a week (depending on usage) inspect the interior of

the rotor chamber for accumulations of foreign matter. Clean as
required (see CLEANING, below), as these accumulations can
result in rotor vibrations.

• Regularly check the stator pad for ease of rotation. Rotate it on the
stator, using light finger pressure, to ensure it moves easily. Also
check for dirt or oil; clean with alcohol* (especially the white
circles on the bottom).

• Regularly check the stator pad for wear; a worn pad is smooth and
shiny on the upper half of the inside surface. A shiny pad has lost
its cushioning effect and will eventually cause rotor failure.
Replace a worn pad.

➠ NOTE
Proper deceleration of the rotor (that is, allowing
the rotor to come to a complete stop before
opening the door) will increase pad life.

CLEANING

➠ NOTE
Before using any cleaning or decontamination
methods except those recommended by the
manufacturer, users should check with the
manufacturer that the proposed method will not
damage the equipment.

Frequent cleaning will ensure proper operation and prolong the life
of the ultracentrifuge. Always clean up spills when they occur to
prevent corrosives or contaminants from drying on component
surfaces. (Be careful not to spill liquid on the instrument where
electrical or mechanical components could become damaged.)
Refer to Chemical Resistances (publication IN-175) for chemical
compatibilities of cleaning materials.

* Flammability hazard. Do not use in or near operating ultracentrifuges.

5-2
 Maintenance

INSTRUMENT INTERIOR AND EXTERIOR

• Clean the instrument exterior surfaces by wiping with a damp

cloth or washing with Beckman Solution 555™ (339555). Dilute
the detergent 10 to 1 with water. Do not use acetone or other
solvents.

• To prevent accumulations of sample, dust, oil, dirt, or other foreign

matter, regularly wipe the channel of the stator, the air jets, and the
brake pin with isopropyl alcohol.*

STATOR ASSEMBLY

Clean the stator assembly when the filter element is replaced, or if the
rotor runs erratically.

! WARNING
Disconnect the power cord and the air supply.

1. Disconnect the air supply at the rear of the instrument by pulling

back the quick-disconnect hose sleeve. The hose may be discon-
nected while it is under pressure, but be careful not to damage
surrounding objects.

2. Remove the stator pad and the brake pin. (Lift the pin out with a
forceps.)

3. Unscrew the two stator screws, then pry the stator off, using the
tools supplied. See Figures 5-1 and 5-2. Note that the slots on the
stator outside rim are used to provide a leverage point for the
stator tools.

4. Remove the three stator O-rings, shown in Figure 5-3. Use a

pointed wooden or plastic tool to avoid scratching the stator.

5. Clean the stator, including the air jets and shaft, with acetone.*

6. Lightly coat the O-rings with silicone vacuum grease (335148)

and reposition them.

* Flammability hazard. Do not use in or near operating ultracentrifuges.

5-3
Maintenance

7. Clean the brake pin and install it in the shaft at the center of the
stator. The pin should move freely up and down in the stator
shaft.

8. To reassemble the stator and pin, align the screw holes then lower
the stator and pin into place. The slot on the end of the stator
must straddle the brake spring in the hole as shown in Figure 5-4.
(The wire direction is indicated by an imaginary line between the
stator screws.) Press the stator down firmly. The brake pin will
bounce up about 3 mm (1/8 in.) above the stator. Lightly touch the
pin to test its motion.

9. Replace the screws. Firmly tighten the screws, alternating until

both are tight.

10. Place the stator pad on the channel of the stator.

11. Reconnect the power cord.

12. To reconnect the air supply, pull back the spring-loaded sleeve of
the quick-disconnect hose and push the sleeve over the fitting at
the rear of the instrument.

LUBRICATION
• Periodically lubricate the threads of the pressure regulator knob
with Spinkote lubricant (306812).

• Lightly coat the three stator O-rings with silicone vacuum grease
(335148) when removed for stator cleaning.

FILTER ELEMENT REPLACEMENT

Expected service life of the filter element is approximately
1400 hours of run time, if the air supply is not excessively oily.
Replace the element when it turns red or when there is an additional
drop of about 67 kPa (10 psig) in the air pressure that reaches the
Airfuge. Instructions for replacing the element are in the Filter
Element Kit (878587).

5-4
 Maintenance

Figure 5-1. Prying Up the Stator Figure 5-2. Lifting Out the Stator.
Keep the stator level to prevent binding.

Brake Pin
Stator Screw
Stator Screw

Stator

Brake
Spring Wire

Stator Slot

Brake Spring
Tension Adjust

Figure 5-3. Stator O-Ring Positions Figure 5-4. Reassembling the Stator and Pin

5-5
Maintenance

BRAKE SPRING WIRE ADJUSTMENT

If the brake pin is flat on the stator surface, either the brake spring
wire is out of position or the brake spring tension is too low.

! WARNING
Disconnect the power cord and the air supply.

1. Remove the stator pad and brake pin. Lift the pin out with a
forceps.

2. Using a flashlight, look straight down the shaft in the center of

the stator. The wire should extend across the center. If the wire is
centered, go to BRAKE SPRING WIRE TENSION ADJUSTMENT,
below. If the wire is not positioned correctly or you do not see the
wire, go on to Step 3.

3. Unscrew the two stator screws, then pry the stator off, using the
tools supplied. See Figures 5-1 and 5-2. Note that the slots on
the stator outside rim are used to provide a leverage point for the
stator tools.

4. Gently push the wire to the center, using a screwdriver blade. If

the wire is too far off center to be engaged by a tool, or if it will
not stay centered, reposition it as follows:

WARNING
Any maintenance procedure requiring
removal of a panel exposes the operator to
the possibility of electrical shock and/or
mechanical injury. Therefore, disconnect the
power cord and the air supply, and refer such
maintenance to trained and qualified service
personnel.

a. Ensure that the power cord and air supply are disconnected,
then turn the instrument on its side and remove the bottom
plate.

b. Position the wire from underneath (see Figure 5-5) so that it

extends across the center of the hole. It may be necessary to
raise the stator slightly so the wire can pass between the stator
and the electromagnet (buzzer). Check the position of the wire
by looking down the hole from the top.

c. Replace the bottom plate. Turn the instrument upright.

5-6
 Maintenance

Figure 5-5. Location of the Brake Spring Wire

from the Ultracentrifuge Bottom

5. Reinstall the stator and brake pin as described under CLEANING,

(Steps 8 through 11 of STATOR ASSEMBLY), above.

BRAKE SPRING WIRE TENSION ADJUSTMENT

If the brake spring wire is centered but the brake pin drops flat on the
stator, the brake spring wire tension requires adjustment. Adjustment
may also be required if the rotor fails to stop.

1. Remove the brake pin and clean it with alcohol, then reinstall it.

2. Turn the BRAKE TENSION screw (see Figure 5-6) clockwise

(decrease), as viewed from the instrument back, until the brake pin
drops to the stator surface.

3. Turn the screw counterclockwise (increase) one-quarter to one-

half turn until the brake pin pops up to its 3-mm (1/8-in.) position
above the stator. (The adjusting screw will turn slightly less than
one full revolution from the fully decreased position.) Stop turning
the screw as soon as the brake pin pops up.

4. Lightly touch the pin. If it bounces freely, the brake is in operating

condition. If the pin motion is sticky, remove it and clean the pin
and shaft with alcohol.

5-7
Maintenance

Figure 5-6. Location of Rear Controls

LEVITATION AIR ADJUSTMENT

If the rotor fails to stop completely or stops too fast, the levitation air
flow may require adjustment.

➠ NOTE
Refer to publication AF-TB-013 for levitation
air adjustment procedures on an instrument
equipped with a digital speed readout accessory.

1. Remove the stator pad and clean it with alcohol,* especially the
white circles on the bottom. Place it on the channel of the stator.

2. Turn the timer on, then back to zero. This will engage the braking
system and hold the brake pin down for 2 minutes while you
complete this adjustment.

3. Place a rotor on the stator pad. Be sure rotor caps or lids are
properly installed. (The rotor does not need to be loaded.)

4. Close the Airfuge door and turn the pressure regulator knob on
the door clockwise until the PRESSURE gauge indicates 30 psig
(207 kPa). The rotor will wobble.

5. Turn the LEVITATION AIR screw on the back panel (see

Figure 5-6) clockwise (decrease) until the rotor stops wobbling.
Then turn the screw slowly counterclockwise (increase) until the
rotor wobbles slightly. Note the position of the screw, then turn it

* Flammability hazard. Do not use in or near operating ultracentrifuges.

5-8
 Maintenance

one-quarter turn further counterclockwise. The rotor will be

wobbling and may be turning in either direction. Leave the
LEVITATION AIR screw at this setting; this is the correct
adjustment.

6. Turn the pressure regulator knob counterclockwise and open the

door. Remove the rotor.

At the end of the 2-minute period, the run light will go out and a
clicking sound can be heard when the brake pin is released.

TIME-DELAY PERIOD ADJUSTMENT

The delay period between the time the timer reaches zero and the run
light goes out may be lengthened or shortened, using the time-delay
knob on the instrument bottom (see Figure 5-7). A setting of about
120 seconds (or 3.5 on older instruments) is optimal.

! WARNING
Disconnect the power cord and the air supply.

Time Delay Knob

Figure 5-7. Location of Time-Delay Knob

5-9
Maintenance

1. Close the Airfuge door and turn the instrument on its side.

2. Turn the time-delay knob counterclockwise to shorten the delay

time, or clockwise to lengthen the delay time.

3. Reconnect the power cord and the air supply and perform a trial
run to check the new setting.

DECONTAMINATION
If the instrument and/or accessories are contaminated with radioactive
or pathogenic solutions, follow appropriate decontamination
procedures as determined by your laboratory safety officer. Refer
to Chemical Resistances (publication IN-175) or contact Beckman
Coulter Field Service to ensure that the decontamination method
does not damage any part of the instrument (or accessories).

STERILIZATION AND DISINFECTION

Instrument surfaces are finished with vinyl paint, the window is made
of polycarbonate, and the front panel is acrylic. Ethanol (70%)* may
be used on these surfaces. See Chemical Resistances for more infor-
mation regarding chemical resistances of instrument and accessory
materials.

While Beckman Coulter has tested these methods and found that they
do not damage the instrument, no guarantee of sterility or disinfection
is expressed or implied. When sterilization or disinfection is a
concern, consult your laboratory safety officer regarding proper
methods to use.

* Flammability hazard. Do not use in or near operating ultracentrifuges.

5-10
 Maintenance

STORAGE AND TRANSPORTATION

To ensure that the instrument does not get damaged, contact Beckman
Coulter Field Service for specific instructions and/or assistance
in preparing the equipment for transport or long-term storage.
Temperature and humidity requirements for storage should meet
the environmental requirements described under SPECIFICATIONS
in Section 1 of this manual.

SUPPLY LIST

➠ NOTE
Publications referenced in this manual can be
obtained by calling Beckman Coulter at 1-800-
742-2345 in the United States, or by contacting
your local Beckman Coulter office.

Contact Beckman Coulter Sales (1-800-742-2345 in the United

States; worldwide offices are listed on the back cover of this manual)
for information about ordering parts and supplies. A partial list of
supplies is given below for your convenience. See the Beckman
Coulter Ultracentrifuge Rotors, Tubes & Accessories catalog
(BR-8101, available at www.beckmancoulter.com) for detailed
information on ordering rotors, tubes, and accessories.

SUPPLIES

Stator pad. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 339610

Stator assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 339609
Brake pin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 339611
Filter assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 339620
Filter mounting kit. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 979436
Forceps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 878446
Level . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 874303
Stator tools (2) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 339641
Filter element kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 878587
Plastic rotor bushing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 339639
Digital Speed Readout Accessory (50-Hz instrument) . . . . . . . . . . . . 347592
Neon lamp for run light. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 979659
Repair Kit 1 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 347597
Spinkote lubricant (2 oz) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 306812
Silicone vacuum grease (1 oz) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 335148
Beckman Solution 555 (1 qt) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 339555

5-11
 WARRANTY FOR THE AIRFUGE® ULTRACENTRIFUGE

Subject to the exceptions and upon the conditions specified CONDITIONS

below and the warranty clause of the Beckman Coulter terms
and conditions of sale in effect at the time of sale, Beckman Beckman Coulter makes no warranty concerning products or
Coulter agrees to correct, either by repair, or, at its election, by accessories not manufactured by it. In the event of failure of
replacement, any defects of material or workmanship which de- any such product or accessory, Beckman Coulter will give rea-
velop within one (1) year after delivery of the Airfuge Ultracen- sonable assistance to the Buyer in obtaining from the respective
trifuge (the product) to the original Buyer by Beckman Coulter manufacturer whatever adjustment is reasonable in light of the
or by an authorized representative, provided that investigation manufacturer’s own warranty.
and factory inspection by Beckman Coulter discloses that such
defect developed under normal and proper use. Beckman Coulter shall be released from all obligations under
all warranties, either expressed or implied, if the product cov-
Some components and accessories by their nature are not ered hereby is repaired or modified by persons other than its
intended to and will not function for one (1) year. If any such own authorized service personnel, unless such repair by others
component or accessory fails to give reasonable service for a is made with the written consent of Beckman Coulter, or unless
reasonable period of time, Beckman Coulter will repair or, at its such repair is, in the sole opinion of Beckman Coulter, minor,
election, replace such component or accessory. What consti- or unless such modification is merely the installation of a new
tutes either reasonable service and a reasonable period of time Beckman Coulter plug-in component for such product.
shall be determined solely by Beckman Coulter.

DISCLAIMER
REPLACEMENT
IT IS EXPRESSLY AGREED THAT THE ABOVE WAR-
Any product claimed to be defective must, if requested by RANTY SHALL BE IN LIEU OF ALL WARRANTIES OF
Beckman Coulter, be returned to the factory, transportation FITNESS AND OF THE WARRANTY OF MERCHANT-
charges prepaid, and will be returned to Buyer with the trans- ABILITY AND THAT BECKMAN COULTER, INC.,
portation charges collect unless the product is found to be SHALL HAVE NO LIABILITY FOR SPECIAL OR CONSE-
defective, in which case Beckman Coulter will pay all transpor- QUENTIAL DAMAGES OF ANY KIND WHATSOEVER
tation charges. ARISING OUT OF THE MANUFACTURE, USE, SALE,
HANDLING, REPAIR, MAINTENANCE, OR REPLACE-
MENT OF THE PRODUCT.
Beckman Coulter, Inc. • 250 S. Kraemer Blvd. • Brea, California 92821
Sales and Service: 1-800-742-2345 • Internet: www.beckmancoulter.com

©2009 Beckman Coulter, Inc.

All rights reserved