B.Pharm.
Seventh Semester (New) Winter 2024
BP701T - Instrumental Methods of Analysis
P. Pages : 3 AG - 2292
Time : Three Hours *4480* Max. Marks : 75
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Notes : 1. All question are compulsory.
2. Answer all question
3. Use of pen Blue/Black ink/refill only for writing the answer book.
1. Multiple choice questions: 20
1) -------------- Radiation has the longer wavelength
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a) Visible b) Ultraviolet
c) IR d) X-ray
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2) In Nephelometer, the intensity of the scattered light is usually measured at ---------
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angle.
a) 44º b) 60º
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c) 70º d) 90º
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3) RF value means the distance travelled by the ------------ to distance travelled by
the ----------.
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a) Solvent, Spot b) Spot, Solvent
c) Spot, Spot d) Solvent, Solvent
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4) Ion-exchange chromatography is used for the separation of ------- molecules.
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a) Polar b) Non-polar
c) a and b d) None of the above
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5) What is principle of Nephelometry?
a) Light scattered b) Light transmitted
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c) a and b d) None of above
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6) In paper chromatography ---------- in filter paper contains moisture which acts
stationary phase.
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a) Liquid b) Cellulose layer
c) Vapor Layer d) Air layer
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7) In silica gel G, G stands for Gypsum which is a:
a) Alkaloid b) Sulfate
c) Alkyl d) None of above
8) Which technique is used to analyze colloidal system?
a) Nephelometry b) Turbidimetry
c) a and b d) None of the above
9) In HPLC degassing can be done by :
a) Helium purging b) Vacuum filtration
c) Ultra sonification d) All of the above
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10) The movement of particles under spatial uniform electric field in a fluid is called:
a) Electrophoresis b) Cataphoresis
c) Both a and b d) None of these
11) What is the range of UV spectroscopy:
a) 10-20 nm b) 50-100 nm
c) 200-400 nm d) None of these
12) Chromophore is responsible for:
a) Colour b) Pigment
c) Emission d) Solubility
13) Halocathode lamp is used in:
a) Flame spectroscopy
b) HPLC
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c) Atomic absorption spectroscopy
d) G.C.
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14) Quantitative principles of UV spectroscopy is based on:
a) Beer’s Law b) Lambert law
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c) a and b d) None of these
15) -------------- Heart of GC
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a) Column b) Pump
c) Radiation source d) Detector
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16) Bolometer detector is used in -----------
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a) U. V. spectroscopy b) I. R. spectroscopy
c) HPLC d) G.C.
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17) Nujol means ----------
a) Mineral oil b) Polymer
c) Crude oil d) Volatile oil
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18) Which material is used to coated cathode:
a) Gold b) Silvera
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c) a and b d) None of these
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19) Nephelometry is similar to --------
a) Fluorimetry b) UV visible spectroscopy
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c) Calorimeter d) NMR
20) Heart of HPLC is ----------
a) Pump b) Solvent
c) Column d) Detector
2. Solve any two long answer questions: 20
i) Explain principles, instrumentation of gas chromatography with any two detectors.
ii) Explain Instrumentation of UV-visible spectroscopy and its applications.
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iii) What is normal phase and reverse phase chromatography ? Explain principles and
instrumentation of HPLC.
3. Short answer questions solve any seven. 35
i) Describe methodology, advantages, disadvantages and application of
chromatography thin layer.
ii) Describe principle, Instrumentation and application of Nepheloturbidometry.
iii) Write about principle and instrumentation of atomic absorption spectroscopy.
iv) Discuss in brief column used in GC.
v) Explain concept of brief principle and instrumentation of AES.
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vi) Explain various spectral shifts in U.V. spectroscopy.
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vii) Describe in detail factor affecting fluorescence and quenching in fluorimetry.
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viii) Explain theory, principle and application of Ion exchange chromatography.
ix) Explain principle, methodology, development techniques and application of paper
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chromatography.
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