BIOMEDIC 3 UROGENITAL SYSTEM
BIOCHEMISTRY OF URINE
dr.Syahrijuita,M.Kes, Sp.THT
BIOCHEMISTRY DEPARTMENT
Faculty of Medicine Hasanuddin University
2017
Biochemistry of Urine
Sub Topic
Function of Kidney and Biochemistry of Urine
Acid-Base Status and Buffer System
Metabolic Acidosis and Metabolic Alkalosis
Kidney Compensation
Metabolism of amino acid, and biosynthesis of
urea,uric acid, creatinine
Composition and Function of vagina secretory,
prostat, vesiculo seminalis and bulbouretralis
secretory
Biochemistry of Urolithiasis
Learning Objectives
Describe function of kidney and biochemistry of urine
Describe acid-base status and buffer system
Describe metabolic acidosis and metabolic alkalosis
Describe kidney compensation
Describe composition and Function of vagina
secretory, prostat, vesiculo seminalis and
bulbouretralis secretory
Describe metabolism of amino acid and biosynthesis
of urea, uric acid, creatinine
Describe biochemistry of urolithiasis
An organic Component
ACID-BASE STATUS
Acids : substances that can donate
hidrogen ions (proton)
Bases: compounds that accept protons
Examples:
HCl H + Cl
NH3 NH4 + OH
Acid-base reactions always involve
conjugate acid-base pairs.
pH VALUE
pH: the H+ concentration in an aqueous
solution
:the negative logarithm of the H+
: - log (0,00000004)
: 7,4
pH of urine: 5,8 ( 4,8 7,5)
because kidney can excrete both acids or
bases.
BUFFER SYSTEMS
Such systems are mixtures of a weak acid
(HB) with its conjugate base (B-) or
A weak base with its conjugate acid
Short-term changes in living organisms
are counteracted.
The main buffer system in the urine
involves ammonium ions (NH4+) and
ammonia (NH3)
METABOLIC ACIDOSIS AND ALKALOSIS
ASIDOSIS & ALKALOSIS METABOLIK
ACID- BASE BALANCE
There are 3 main system to manage
(H+)in body fluid and to prevent
acidosis and alkalosis:
1. Buffer Systems, to prevent (H+) over
2. Respiratoric System, to excrete CO2
3. Kidney, to excrete acid/base
Buffer system of the plasma
KIDNEY COMPENSATION
There are 3 mekanism to manage ion
Hidrogen concentration at extracellular
fluid:
1.Secretion of Hidrogen ions
2.Reabsorption of bikarbonat ions
3.Production of new bikarbonat ions
Kidney control acid-base balance with
excrete both acid or base in urine.
Respiratoric Acidosis: hipoventilation and
increase of P CO2
Respiratoric alkalosis: hiperventilation and
decrease of P CO2
Kidney compensation:
Acidosis: increase of ion hydrogen excretion
increase Bicarbonat extracelular fluid
Alkalosis: decrease of ion hydrogen secreation
at tubulus.
decrease of bicarbonat extraceluler
fluid.
DIAGNOSTIK ASAM BASA
GANGGUAN ASAM BASA KOMPLEKS &
PENGGUNAAN NOMOGRAM ASAM BASA
UNTUK DIAGNOSTIK
ALIRAN NITROGEN
SECRETORY OF VAGINA ,SECRET OF
PROSTAT, SECRET OF VESICULA
SEMINALIS, SECRET OF
BULBOURETRALIS
COMPOSITION AND FUNCTION OF
VAGINA SECRETORY
COMPOSITION AND FUNCTION OF :
- PROSTAT SECRETORY
- VESICULO SEMINALIS SECRETORY
- BULBOURETRALIS SECRETORY
SECRETORY OF VAGINA
Secretory fluid from vagina and Bartollini
secretion combine with bacteries and
production.
Fuctions of vagina secretory ,are :
1. To cleaning its self
2. As lumbricans
3. Immun from infections,etc
Normally, it is white- grey or yellowist ,
transparan if dried
The normal of vagina secretory
Contents microorganisms,means 6
species bacteries
The normal flora aerobic
>>> Lactobacillus carispatus and
Lactobacillus jensenii.
prevent other bacteries / anaerobic
growth by Hydrogen Perokside
production
The normal kompositions of vagina
secretory : water, electrolit, mikroorganism,
epitelial cells and organic compound like :
fatty acid, protein, and carbohidrat .
pH of vagina secretory is < 4,5
If pH is base will reduce the normal flora exp
: by douchess and frequent of coitus
eas
for repeat of infections
3 sex secretory in man
1. Prostat secretory : the white acid fluid
2. Vesico seminalis secretory : to product
base fluid to netralization the acid
condition in vagina and to prepare
fruktosa as energy of sperma for motility
3. Cowper secretory : to netralization acid
in urethrae
Cemen ( Air Mani)
The 3 th sex secretory result fluid for care
sperma life and fluid for motility
60 - 100 million sperma in 1 cc cemen
pH of cemen is 7,2 volume 3 - 5 cc
Colour : milky white -yellowist
Secretory of Prostat
Prostat only one and product the white fluid
leke milk- grey
Secretory of prostat is 30% volume of cemen
The contens :enzim proteolytic and
anticoagulans,citrat acid as sperma
nutrition,kolesterol,fosfolipid and salts
This secretory prevent sperma from high
pressure in uretrae and vagina
Testesteron is regulatory hormon
Secretory of Vesiculo Seminalis
The secret fluid from Vesiculo seminalis
behind of VU
This yellow secret as nutrition for energy
of sperma
pH 7,3
60% total volume of sperma
Contents : mucus,fructosa, ascorbat acid,
protaglandin dan coagulation enzim
Secretory of Bulbourethralis=
Cowper
5% volume of cemen,clearly fluid
pH is base
Function :- netralization acid urine in
urethrae
- Lumbricant fluid in urethrae
Found : every Mamalian ( male ) except :
dog
BIOCHEMISTRY OF
UROLITHIASIS
Calculus >>> Kidney
Analysis of calculus:
75% Calsium Oxalat/ + Calsium
Phosphat
15% Magnesium Ammonium Phosphat
10% Uric acid/ Cysteine
2,5% organic material:mucoprotein
PREDISPOSITION FACTOR
1. FAMILIAL
2. SEX, >> MAN
3. CONCENTRATION OF CALCULUS
MATERIAL
4. INFECTION
5. CRONIC DISEASES
6. ACID/BASE OF URINE,ETC
Calsium calculus of kidney:
70% hipercalsiuria
25% abnormalitas of kidney
Hipertiroidism : hipercalsemia+hipercalsiuria
Recurrence of infectionbase
urinemagnesium ammonium phosphat
calculus
Gout, Leukemia:>>uric acid calculus
Acid urineuric/ cysteine calculus
Cysteine calculus.>> genetic anomali of amino
acid transport at kidney
Mucoprotein is the basic organic material of
calculus
