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Microbiology STAINING

Staining techniques are used to visualize microorganisms under a microscope. There are several types of stains that are used for different purposes: basic dyes stain all microorganism components, acidic dyes stain only certain components, and special stains are used to distinguish specific structures like bacterial spores or capsules. Common staining techniques include simple staining to highlight total bacteria count, Gram staining to differentiate bacteria types, acid-fast staining for mycobacteria, and spore or capsule staining for specific visualization.

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129 views21 pages

Microbiology STAINING

Staining techniques are used to visualize microorganisms under a microscope. There are several types of stains that are used for different purposes: basic dyes stain all microorganism components, acidic dyes stain only certain components, and special stains are used to distinguish specific structures like bacterial spores or capsules. Common staining techniques include simple staining to highlight total bacteria count, Gram staining to differentiate bacteria types, acid-fast staining for mycobacteria, and spore or capsule staining for specific visualization.

Uploaded by

Kai
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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STAINING

TECHNIQUES

1
Because microbial
cytoplasm is usually
transparent, it is necessary
to stain microorganisms
before they can be viewed
with the microscope.
BASIC DYE

ACIDIC DYE

3 3
STAIN

Staining can be performed with The differential stain technique A special stain technique is used
a single stain. distinguishes two kinds of to examine bacterial spores
organisms.
SIMPLE POSITIVE STAINING- crystal
violet or methylene blue

SIMPLE NEGATIVE STAINING- nigrosin or


Congo red
and ears;
• For visualization of morphological shape and arrangement of microorganisms
• To highlight the total count of bacteria
• Ex. Loeffler’s Methylene Blue, Dilute Carbol Fuschin, Crystal Violet
Gram Staining
This procedure separates bacteria into two
groups: Gram positive bacteria and
Gram negative bacteria.

Gram negative bacteria give


pink color
Gram positive bacteria give
purple color

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PROCEDURE

Come In And Stain


1 2 3 4 5

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This technique differentiates species of Mycobacterium
from other bacteria. Because the cell wall is resistant to
water-based stains, acid-fast organisms require a
special staining technique

acid fast bacteria appear bright red

non-acid fast bacteria appear blue

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SPORE STAIN

15
CAPSULE STAIN

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FLAGELLA STAIN

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OTHER STAINING
TECHNIQUES

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Wheatley Positive Staining Negative Staining Gomori Methenamine Periodic acid-Schiff
Trachoma Stain of Viruses of Viruses Silver (GMS) Staining (PAS ) Staining

Ex. Wheatley’s
stain for Chemical: Chemical: Ex. GMS staining Ex. Candida on
intestinal Uranyl Acetate Uranyl Acetate of Aspergillus PAS Stain
amoeba
References

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THANK YOU

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