Back cross
and
test cross
In backcross, the F1 is crossed with
one of the parents or genetically
identical individual to the parent
Parent F1-Tall Tall
Phenotype
Genotype
Tt X TT
Gametes T t T T
F2
F T t
M
TT Tt
T Tall Tall
T
TT Tt
Tall Tall
Parent Tall Dwarf
Phenotype
Genotype
Tt X tt
Gametes T t t t
F2
F T t
M
Tt tt
t Tall Dwarf
t
Tt tt
Tall Dwarf
In test cross, a dominant phenotype
is crossed with the homologous
recessive genotype in order to
discriminate between homologous
dominant and heterozygous
genotypes
Parent Tall Dwarf
Phenotype
Genotype
Tt X tt
Gametes T t t t
F2
F T t
M
Tt tt
t Tall Dwarf
t
Tt tt
Tall Dwarf
F2 phenotypic and genotypic ratio is 1:1
The main difference between test cross and the
backcross is that test cross is used to
discriminate the genotype of an individual which
is phenotypically dominant whereas a backcross
is used to recover an elite genotype from a
parent which bears an elite genotype
All test crosses are
back cross but all back
crosses are not test
cross
Parent Yellow round seed X Green wrinkled
Phenotype
Genotype
YYRR X yyrr
Gametes
YR yr
F1
YyRr
Yellow round
Parent Yellow round seed X Green wrinkled
Phenotype
Genotype
YyRr X yyrr
Gametes
YR Yr yR yr yr
F2
YyRr Yyrr yyRr yyrr
Yellow round Yellow Wrinkled Green round Green Wrinkled
YyRr
Yellow round