Seminar on
Phytosomal Drug Delivery For
Wound Healing
Presented By: Guided By:
Sharvari D. Korde. Dr.S.N. Shrotriya
1ST yr. M.Pharm Sinhgad College Of
Department of pharmaceutics Pharmacy,Vadgaon Bk.
Roll no.509
Content
• Introduction to phytosomes as a novel drug delivery
• Phytosomal formulation
• Methods of preparation
• Characterization
• Benefits of phytosomal drug delivery
• Wound healing
• Phases of wound healing
• Stages of wound healing
• Case study (in detail)
• Reference
sharvari korde 07/19/2021 2
Introduction: Phytosomes (Herbosomes)
• Definition : “phyto” plant
“somes” cell like structure
• Advanced form of herbal formulation
• Properties of phytosomes : chemical and
biological
Tripathy.S, Patel.D, Baro.L, Nair.S,[2013]. Review on phytosomes, their characterization, advancements and
potential for transdermal application. Journal of drug delivery and therapeutics.
Sharvari korde 07/19/2021 3
Phytosomal formulation
Phospholipid
Solution of
(dissolved in a phospholipid in Drying /solvent
organic solvent organic solvent with evaporation
containing drug drug extract
extract)
Phytosome
suspension Formation of thin
Hydration
film
Patel.J, Patel.R, Khambholja.K, Patel.N,[2009].An overview of phytosomes as an advanced
drug delivery system.Asian journal of pharmaceutics[14th April 2009].
sharvari korde 07/19/2021 4
Different additives used in the formulation of
phytosomes
Phospholipids: Soya phosphatidyl choline, Egg phosphatidyl choline ,
phosphatidylcholine, phosphatidylserine, etc.
Aprotic solvents: Dioxane, Ethyl acetate, Methylene chloride
Protic solvents: Ethanol
Solvent: Dichloromethane(DCM)
Anti-solvent: N-hexane and non- solvent i.e. aliphatic hydrocarbon
Khan.J, Alexander.A, Ajazuddin, Saraf.S, Saraf.S,(2013). Recent advances and future prospects of phyto-
phospholipid complexation technique for improving pharmacokinetic profile of plant actives. Journal of
controlled release. Elsevier publication. [6th March 2013]
sharvari korde 07/19/2021 5
Methods of Preparation
Antisolvent
precipitation technique
Rotary evaporation
technique
Solvent evaporation
method
Singh.R.P, Narke.R,[2015].Preparation and evaluation of phytosome of lawsone.International journal
of pharmaceutical sciences and research .[1st December 2015].
sharvari korde 07/19/2021 6
Anti-solvent precipitation technique
Reflux with 20ml
Drug +soya Concentrate
dichloromethane
lecithin mixture to 5-10 ml
at 60℃ for 2hr.
Filter the ppt
formed dry, crush
Add hexane 20 ml
and pass through
#100
Singh.R.P, Narke.R,[2015].Preparation and evaluation of phytosome of lawsone.International journal of
pharmaceutical sciences and research .[1st December 2015].
sharvari korde 07/19/2021 7
Rotary evaporation technique
Drug +soya Ppt obtained Dry
lecithin
Dissolved in
Add n-hexane Pass through
30ml of
with stirring mesh
tetrahydrofuran
Stirring for 3hr
at temperature Thin film is Phytosome
not exceeding formed formed
40℃
Singh.R.P, Narke.R,[2015].Preparation and evaluation of phytosome of lawsone.International journal of
pharmaceutical sciences and research .[1st December 2015].
sharvari korde 07/19/2021 8
Solvent evaporation method
Drug +soya lecithin
Refluxed with 20ml of acetone at temperature 50-60℃ for 2 hr.
Concentrated mixture to 5-10 ml
Obtain the precipitate
Filter and collect
Phytosomes obtained
Singh.R.P, Narke.R,[2015].Preparation and evaluation of phytosome of lawsone.International journal of
pharmaceutical sciences and research .[1st December 2015].
sharvari korde 07/19/2021 9
Characterization of phytosomes
Characterization
FTIR SEM XRD DSC
Fourier-transform IR Scanning electron Differential scanning
spectroscopy X-ray diffraction
microscope analysis calorimetry
Tripathy.S, Patel.D, Barob.L, Nair.S, [2013]. A review of phytosomes, their characterization, advancement
and potential for transdermal application.Journal of drug delivery and therapeutics.
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Benefit Of Phytosome Complex
Khan.J,Alexander.A,Ajazuddin,Saraf.S,Saraf.S[2013].Recent advances and future prospects of phyto-phospholipid complexation
technique for improving pharmacokinetic profile of plant actives. Journal of controlled release. Elsevier publication.[6 th march
2013].
Sharvari korde 07/19/2021 11
Wound Healing
Phases Of Healing
Inflammatory Proliferative Maturational
(reactive) (regenerative) (remodeling)
Haemostasis Epithelial migration Contraction
Inflammation Proliferation Scarring
Maturation Remodeling
Muzumdar.A, Dwivedi.A, Preez.J.L.D, Plessis.J.D,[2015].In-vitro wound healing and cytotoxic effects of sinigrin-
phytosome complex.International journal of pharmaceutics. Elsevier publication[17 th December 2015]
sharvari korde 07/19/2021 12
Chadwick S, Heath R, Shah M. Abnormal pigmentation within cutaneous scars: a complication of wound healing. Indian journal of plastic surgery: official
publication of the Association of Plastic Surgeons of India. 2012 May;45(2):403.
sharvari korde 07/19/2021 13
Interaction of phytosome complex with
biological membrane
Khan.J, Alexander,A, Ajazuddin, Saraf.S, Saraf.S [2013].Recent advances and future prospect of phyto-phospholipid complexation technique for
improving pharmacokinetic profile of plant actives. Journal of controlled release: Elsevier publication.[6 th march 2013]
sharvari korde 07/19/2021 14
Case Study
Muzumdar.A, Dwivedi.A, Preez.J.L.D, Plessis.J.D.[2015].In-vitro wound healing and cytotoxic effects of sinigrin-phytosome
complex.International journal of pharmaceutics 498(2016)283-293. Elsevier publication[17th December 2015]
sharvari korde 07/19/2021 15
Preparation Of Sinigrin-Phytosome
Complex
Phosphatidylcholine + Stirr well and evaporate
sinigrin (1:1) 5 ml of DCM
DCM
Thin film
N-Hexane
Thin film hydration + Stirr well and wait formed
(5ml)
sonication
PHYTOSOME
COMPLEX
Muzumdar.A, Dwivedi.A, Preez.J.L.D, Plessis.J.D,[2015]. In-vitro wound healing and cytotoxic effects of sinigrin-phytosome
complex.International journal of pharmaceutics 498(2016)283-293. Elsevier publications.[17 th December 2015].
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In-vitro toxicity study
• Cytotoxicity test of sinigrin , sinigrin-phytosome complex and
phytosome alone.
• A-357(Human malignant melanoma) and HaCaT (human
immortalized keratinocytes) (2×104) cells were used.
• Cells were treated with different concentrations of phytosome alone,
sinigrin alone and sinigrin-phytosome complex.
(0.048mg/ml,0.072mg/ml,0.096mg/ml,0.14mg/ml resp.)
• MTT assay
• Cell viability testing
• Cell migration assay
Muzumdar.A, Dwivedi.A, Preez.J.L.D, Plessis.J.D,[2015]. In-vitro wound healing and cytotoxic effects of sinigrin-phytosome complex. International
journal of pharmaceutics 498(2016)283-293. Elsevier publications. [17th December 2015].
sharvari korde 07/19/2021 17
Cell viability testing
Muzumdar.A, Dwivedi.A, Preez.J.L.D, Plessis.J.D,(2015). In-vitro wound healing and cytotoxic effects of sinigrin-phytosome
complex. International journal of pharmaceutics 498(2016)283-293. Elsevier publications. [17th December 2015].
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Wound closure
Muzumdar.A, Dwivedi.A, Preez.J.L.D, Plessis.J.D,(2015). In-vitro wound healing and cytotoxic effects of sinigrin-phytosome
complex. International journal of pharmaceutics 498(2016)283-293. Elsevier publications. [17th December 2015].
sharvari korde 07/19/2021 19
Cell migration assay
Muzumdar.A, Dwivedi.A, Preez.J.L.D, Plessis.J.D,(2015). In-vitro wound healing and cytotoxic effects of sinigrin-phytosome
complex. International journal of pharmaceutics 498(2016)283-293. Elsevier publications. [17th December 2015].
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Observations at 0.07gm/ml conc.
Muzumdar.A, Dwivedi.A, Preez.J.L.D, Plessis.J.D(2015). In-vitro wound healing and cytotoxic effects of sinigrin-phytosome complex.
International journal of pharmaceutics: Elsevier publications. [17th December 2015].
sharvari korde 07/19/2021 21
Observations at o.14mg/ml conc.
Muzumdar.A, Dwivedi.A, Preez.J.L.D, Plessis.J.D,(2015). In-vitro wound healing and cytotoxic effects of sinigrin-phytosome
complex. International journal of pharmaceutics 498(2016)283-293. Elsevier publications. [17th December 2015].
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In-Vitro Cell Migration Assay
• Increased in concentration of
sinigrin (0.14mg/ml) there is
increased in percentage cell
migration to 97% and sinigrin-
phytosome complex to 145% cell
migration.
• Only sinigrin and only
phytosome conc. May have lower
percentage of cell migration than
sinigrin-phytosome complex.
Muzumdar.A, Dwivedi.A, Preez.J.L.D, Plessis.J.D,(2015). In-vitro wound healing and cytotoxic effects of sinigrin-phytosome complex.
International journal of pharmaceutics498(2016)283-293. Elsevier publications. [17th December 2015].
sharvari korde 07/19/2021 23
Case Study Conclusion
At higher conc.0.14mg/ml(42hr.) sinigrin-phytosome
complex completely cured wound (100%)
Sinigrin alone displayed only 71% wound healing
Sinigrin-phytosome complex enhanced the cytotoxic activity
than sinigrin alone
Muzumdar.A, Dwivedi.A, Preez.J.L.D, Plessis.J.D,[2015]. In-vitro wound healing and cytotoxic effects of sinigrin-phytosome
complex. International journal of pharmaceutics: Elsevier publications. [17th December 2015].
sharvari korde 07/19/2021 24
Overall conclusion
• Phytosomes can enhance the bioavailability of drug.
• Phytosomal drug delivery can enhanced the absorption of herbal
constituents.
• Entrapment efficiency is high in the phytosomal drug delivery.
• Phytosomal drug delivery on wound shows greater healing activity.
• Phytosomal drug delivery effective in transdermal drug delivery.
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Reference
•Muzumdar.A, Dwivedi.A, Preez.J.L.D, Plessis.J.D,(2015). In-vitro wound healing and
cytotoxic effects of sinigrin-phytosome complex. International journal of pharmaceutics
498(2016)283-293. Elsevier publications. [17th December 2015].
•Khan.J, Alexander.A, Ajazuddin, Saraf.S, Saraf.S, Recent advances and future prospects
of phyto-phospholipid complexation technique for improving pharmacokinetic profile of
plant actives. Journal of controlled release: Elsevier publication. [6th March 2013]
•Chadwick S, Heath R, Shah M. Abnormal pigmentation within cutaneous scars: a
complication of wound healing. Indian journal of plastic surgery: official publication of
the Association of Plastic Surgeons of India. 2012 May;45(2):403.
•Tripathy.S, Patel.D, Baro.L, Nair.S, [2013].A review on phytosomes , their
characterization, advancement and potential for transdermal application. Journal of
drug delivery and therapeutics
•Patel.J, Patel.R, Khambholja.K, Patel.N,[2009].An overview of phytosomes as an
advanced drug delivery system.Asian journal of pharmaceutics[14th April 2009].
sharvari korde 07/19/2021 26
sharvari korde 07/19/2021 27
