BC-6000 service training
clincal basic
IVD Global Technical Support Dept.
Version ： V1.0
Number: MXQ-18032-BC-6000
© 2018 Mindray Confidential
Clinical basic knowledge
2 © 2018 Mindray Confidential
RET·NRBC Introduction
RBC System
Proerythroblast Prorubricyte Polychromatic Metarubricyte Reticulocyte Mature erythrocyte
Nucleated Red Blood Cells
3 © 2018 Mindray Confidential
Reticulocyte
RET （ 0.025~0.075×1012/L ）
Between Metarubricyte and Mature erythrocyte.
Important indicator for Anemia treatment
4 © 2018 Mindray Confidential
Reticulocyte---Morphology
HFR ： High Fluorescent RET
MFR ： Medium Fluorescent RET
LFR ： Low Fluorescent RET
TYPE I TYPE II TYPE III TYPE IV
HFR MFR LFR
5 © 2018 Mindray Confidential
Ret calculation
Re t #
Re t(%) 100
RBC #9
6 © 2018 Mindray Confidential
NRBC -- Nucleated red blood cell
NRBC （ 0~0.02×109/L ）
There will be no NRBC in normal
human peripheral blood, except for
neonate and fetus.
Because NRBC have cell nucleus
inside, it is always identified as
WBC.
7 © 2018 Mindray Confidential
NRBC-- calculation
NRBC #
NRBC(%) 100
WBC #
8 © 2018 Mindray Confidential
Fluorescence
Feature of fluorescence
Lower intensity
Longer wavelength Fluorescence
Affect by temperature Excitation light
9 © 2018 Mindray Confidential
Fluorescence -- nuclear acid
Dye + Nuclear ACID Compound
Key questions:
a) How does the dye get into cell ? --- Affect the combination of dye and
nuclear acid
b) How much is the nuclear acid? --- Affect the target quantity of the dye
c) The density of the chromatin? --- Affect the efficiency of the bonding
process
d) The concentrate of dye? --- Affect the intensity of the fluorescence
e) Higher temperature, lower fluorescence intensity
f) Reaction time
g) Other factors
10 © 2018 Mindray Confidential
Cell maturation
Fluorescent
幼稚
Immature Mature Intensity
成熟
Strong Weakness
Cell Size Big Small 强 弱
Nucleo- Strong Weakness
Big Small 强 弱
cytoplasmic ratio
Nuclear chromation Strong Weakness
structure 强 弱
Loose Tight
Nuclear acid Strong Weakness
More Less 强 弱
quantity
11 © 2018 Mindray Confidential
Version Information
Version Editor Date Description
Number
1.0 Marcus 2018-6-28 First release
12 © 2018 Mindray Confidential
Review Information
Version Reviewed Review Date Revision Details
Number by
1.0 Luke 2018-6-30 First release
13 © 2018 Mindray Confidential
Thank you!
14 © 2018 Mindray Confidential
