RAMAN SPECTROSCOPY
BY
Dr. Bilal Ahmad Bhat
Assistant Professor Physics
GDC Sopore
Plan of presentation
Why NSD is celebrated
Why NSD is celebrated on February 28
What is Raman Effect
Lines in Raman Spectrum
Quantum theory of Raman Spectra
Applications of Raman Spectroscopy
Raman spectra of my Research work
Why NSD is celebrated
To spread a message about the importance of science
in our day to day life.
To Present all the activities, efforts and achievements
in the field of science for human welfare.
To discuss New technologies for the development in
the field of science.
To provide New opportunities to the citizens of country
To encourage the people to take part in new scientific
inventions
TO popularize science and technology.
Why NSD is celebrated on February 28
National Science Day is celebrated in
India on 28 February each year to mark
the discovery of the Raman effect by
Indian physicist Sir C. V. Raman on 28
February 1928.
For his discovery, Sir C.V. Raman was
awarded the Nobel Prize in Physics in
1930.
The government of India elected 28
February as National Science Day (NSD) in
1986.
What is Raman Effect?
It is an inelastic scattering that refers to change in the Frequency /Wavelength of
light when passed through some medium.
Lines in Raman Spectrum
1. Rayleigh Line
Ѵ = Ѵi
2. Stokes Lines
Ѵ < Ѵi
3. AntiStokes Lines
Ѵ > Ѵi
Quantum theory of Raman Spectra.
According to quantum theory when a beam of monochromatic light of frequency Ѵi in
the form of photons each of energy h Ѵi strikes a molecule of sample.
Elastic Collision Inelastic Collision
No loss or gain of Energy by Photon
Rayleigh Line
Energy Loss Energy Gain
Stokes lines Antistokes Lines
Energy Level Diagram
E v = h ν (v + 1 2) v = 1,2,3,.....
Selection Rule
∆v =0, ±1, ±2,….
Block Diagram of Raman Spectrometer
Applications of Raman Spectroscopy
1.In medical field, Raman spectroscopy is used to diagnose
various disease tumor, cancer , Blood infection etc.
2. Biomolecules can also be directly sensed with Raman
spectroscopy, that enables a new understanding of DNA, proteins,
lipids, and carbohydrates in biological organisms.
Raman spectra DNA
3. In material science to determine the structure of molecules , bonds, crystallinity etc of
samples.
Raman Spectra of My Research work samples Carried at IUAC New .