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Packed Cell Volume Heamatology

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60 views9 pages

Packed Cell Volume Heamatology

Uploaded by

olaifatomilola9
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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CHAPTER 3

Medical Laboratory Practical


HEAMATOLOGY

BY: ABDULMALIK A.O


DEPARTMENT : MEDICAL LABORATORY SCIENCE
FACULTY: NURSING AND ALLIED HEALTH SCIENCE
PACKED CELL VOLUME (PCV)

PACKED CELL VOLUME (PCV)


 A packed cell volume (PCV) test is a routine blood test that analyses
and measures the proportion of red blood cells in a blood sample.
 It is also alternatively known as a hematocrit test and is expressed as
a percentage of packed RBCs by volume in a blood sample, from
which the amount of RBCs present in the body can be inferred
PRINCIPLES AND PROCEDURE FOR PCV TEST

Principles: The PCV test is performed in a laboratory by centrifuging


a blood sample.
 During centrifugation, the blood cells settle at the bottom of the
centrifuge tube while the plasma rises to the top.
 The percentage of red blood cells in the blood sample can be
determined by measuring the height of the packed RBCs at the
bottom in relation to the total height of the sample.
REAGENT/MATERIAL

 Capillary Tube, Plasticine, Lancet, EDTA Bottle, Needle & Syringe,


Dry Swab, Alcohol pad, Tourniquet, Adhesive pad, latex gloves.
PROCEDURE

 fill the plain capillary tube with well – mixed venous blood collected in EDTA or
heparinized capillary tube with blood from a freely flowing capillary puncture.
 seal one end of the capillary tube in a Bunsen flame/with plasticine.
 place the capillary in the micro-hematocrits centrifuge, and screw on the safety
cover.
 close the lid and spin at 12,000 rpm for five minutes
 place the spun tube into the specially designed hematocrits Reader and read off
the PCV as percentage.
 Sample: whole blood collected in EDTA or from a capillary puncture.
Normal Ranges:

PCV RANGE
Men: 37 - 50%

Women: 34 - 47%

Cord Blood: 44 - 64%

Children: 32 - 45%
USES OF PCV TEST
 A PCV test primarily measures the percentage of RBCs in a sample
of blood.
 It is usually performed to diagnose or rule out blood conditions
such as anemia or polycythemia in patients.
 Doctors may also recommend the PCV hematocrit test to evaluate
the response to blood transfusions or determine the effectiveness of
treatments for certain blood conditions.
 Overall, a PCV test provides a thorough assessment of a person's
health and their risk of developing certain medical conditions. PCV
test results are expressed in percentages.
 If the PCV blood test shows a reading of 50%, it means that in 100
ml of blood, red blood cells occupy 50 ml by volume.
 Any increase or decrease in this reading may indicate various health
conditions and problems, some of which may be chronic
FACTORS AFFECTING THE ACCURACY OF PCV

 Poor sample collection


 Incorrect blood/anticoagulant ratio
 Incorrect centrifugation
 Technical error
 Wrong reading due to error of parallax
 Cell lyse/destruction due to flame burnt during sealing.
CONCLUSION

 Pcv test can be used for diagnosing certain blood disorders or for
monitoring the overall health of patients.

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