SIWES DEFENSE
PRESENTATION
BY: UDOMA MAGDALENE OGHENEKPAROBO
MATRIC NUMBER: UAT20/03/01/1355
DEPARTMENT: Chemical Sciences
PROGRAMME: BSc. Biochemistry
INSTITUTION: University Of Africa, Toru-orua
INDUSTRIAL PLACEMENT BASE: Godreigns Clinic And
Maternity Jeddo Okpe, Delta State.
DEFENSE TOPIC: INSTITUTION SUPERVISOR:
PACKED CELL VOLUME - PCV DR. BENNETT VICTORIA
SWIPE
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FORWARD
TOPIC:
PACKED CELL
VOLUME - PCV
P R E S E N TAT I O N F O C U S :
AIM AND INTRODUCTION/PRINCIPLES, CAUSES
O F LO W A N D H I G H H E M AT O C R I T ( H C T ) ,
M AT E R I A L S , P R O C E D U R E S , R E S U LT S ,
C O N C LU S I O N / I N F E R E N C E , A N D R E F E R E N C E .
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UDOMA M.O.SLIDE 2 SWIPE FORWARD
INDUSTRY BASE ORGANOGRAM
BOARD OF
DIRECTOR
HEALTH CHIEF
SECRETARY ACCOUNTANT
ACCOUNT SALARIES
REGISTARY MAINTANANCE
FINANCE REPORT
SECURITY
HOD CLINIC CLINIC
SERVICE MATRON
PHARMACY LABORATORY CONSULTANT NURSING
RECEPTIONS
WARDS AND
ICU AND
UNITS
EMERGENCY
PROJECT AND
TECHNICAL
TRANSPORT REAPAIR
ELECTRICAL FEUL
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ACTIVITIES DONE
DURING SIWES
Malaria Parasite Test Full Blood Count (FBC)
Blood Glucose Test Blood Grouping Test
Packed Cell Volume (PCV) Genotyping Test
Liver Function Test (LFT) Pregnancy Test
Lipid Profile Test Hormonal Profile Test
Urinalysis E/U/CR etc.
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AIM OF PCV TEST
Also known as the haematocrit test, the PCV or
Packed cell volume test.
It’s a test done to diagnose polycythaemia,
dehydration or anaemia in certain patient.
It is generally a part of the full blood count test
that is used to estimate the need for certain blood
transfusions and monitor the response to
treatment.
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PRINCIPLE
OF PCV TEST
The PCV test measures how much of blood
consists of red blood cell. i.e; measures the
volume percentage of red blood cells in the blood
If the PCV returns a reading of 50%, it means
that 50 mL of the red blood cells are present in
exactly 100 mL of blood.
If the RBC number increases, then the total
reading of the PCV is also up.
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MATERIALS
NEEDED
Anticoagulated whole blood(normally in EDTA
bottle)
Bunsen burner
PCV/Hematocrit reader
Hematocrit centrifuge
Capillary tubes
Disposable gloves
Tissue paper
Clinical waste bin
Timer
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TEST PROCEDURE:
Hematocrit is diagnosed using automated analyzer or the
manual method (spun hematocrit or spun crit).
SPUN HEMATOCRIT” METHOD
A thin capillary tube is filled with whole blood
sample and the end is sealed with flame.
The tube is then placed in a micro hematocrit
centrifuge to be centrifuged for five(5) minutes using
a timer
Note: The red cells collect at the bottom and form a
red column and are separated from the straw-
colored serum column by a small area composed of
white blood cells. The tube is read using micro
hematocrit reader. Bull, B. S., Koepke (2000).
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PCV TEST RESULT:
The height of the total blood in the capillary tube
(red cells, white cells and serum) equals 100%. The
height of the red cell column divided by the height of
the total fluid in the capillary tube equals the
hematocrit (% of RBC of the total blood volume).
Abnormally low PCV refers to anemia and
abnormally high PCV refers to polycythemia.
Ranges
Normal values for hematocrit test vary accordingly
to age, sex, pregnancy, altitude where people live
and even vary slightly between various laboratory.
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REFERENCE RANGE
The following are reported ranges of normal
hematocrit levels:
New born: 55%-68%
Children: 29%-41%
Adult men: 42%-54%
Adult female: 38%-46%
TREATMENT
The treatment of high and low hematocrit depends on the
underlying causes, the HCT level,
and the overall health status of the individual. Most people
are not treated with medications
if result is slightly above or below the normal levels. Some
patients with very low HCT may require intravenous iron.
Some patients with very high hematocrit due to diseases,
such as polycythaemia, may require bloodletting (blood
removal).
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REFERENCE
• Bull, B. S., Koepke, J. A., Simson, E., & Van Assendelft,
O. W. (2000). Procedure for determining packed cell
volume by the microhematocrit method; approved
standard. NCCLS Document H7-A3, 20(18), 1-8.
• https://www.portea.com/labs/diagnostic-tests/packed-cell-
volume-test
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THANK
YOU FOR
LISTENING
THE END
