Introduction to Bacteria




USDA NIFSI Food Safety in the Classroom©
 University of Tennessee, Knoxville 2006
What are bacteria?
              • Single celled organisms
                                                                  E. Coli O157:H7
              • Very small                                        can make you
                                                                  very sick.


              • Need a microscope to see
              • Can be found on most
                materials and surfaces
                                                                        Streptococcus
                       – Billions on and in your                        can cause strep
                                                                        throat.
                         body right now
                                                   This E. coli helps
USDA NIFSI Food Safety in the Classroom©
 University of Tennessee, Knoxville 2006           you digest food.
What do they look like?
                     • Three basic shapes                              Bacilli
                              – Rod shaped called bacilli
                                (buh-sill-eye)
                              – Round shaped called
                                cocci (cox-eye)               Cocci
                              – Spiral shaped

                     • Some exist as single
                       cells, others
                     cluster together
USDA NIFSI Food Safety in the Classroom©   Cluster of cocci           Spiral
 University of Tennessee, Knoxville 2006
Bacteria are ALIVE!


                                           • What does it mean
                                             to be alive?
                                             – They reproduce
                                               (make more of
                                               themselves)
                                             – They need to eat


USDA NIFSI Food Safety in the Classroom©
 University of Tennessee, Knoxville 2006
How do bacteria
                     reproduce?
                     • Grow in number not in size
                              – Humans grow in size from child to adult

                     • Make copies of themselves by
                       dividing in half
                              – Human parents create a child




USDA NIFSI Food Safety in the Classroom©
 University of Tennessee, Knoxville 2006
How do bacteria eat?
              • Some make their own food from
                sunlight—like plants                                           Photosynthetic
                                                                                 bacteria

              • Some are scavengers
                       – Share the environment around them
                                • Example: The bacteria in your stomach
                                  are now eating what you ate for breakfast
                                                                              Harmless bacteria
              • Some are warriors (pathogens)                                  on the stomach
                                                                                    lining
                       – They attack other living things
                                • Example: The bacteria on your face can
                                  attack skin causing infection and acne
                                                                              E. Coli O157:H7
USDA NIFSI Food Safety in the Classroom©
 University of Tennessee, Knoxville 2006                                       is a pathogen
What is a pathogen?
                     • Bacteria that make you sick
                              – Why do they make you sick?
                                       • To get food they need to survive and
                                         reproduce

                              – How do they make you sick?
                                       • They produce poisons (toxins) that result in
                                         fever, headache, vomiting, and diarrhea
                                         and destroy body tissue

USDA NIFSI Food Safety in the Classroom©
 University of Tennessee, Knoxville 2006
Where do you get a
                                                  pathogen?

                  Indirect contact

                             • Contact with people who are sick
                                      – Direct or indirect

                             • Food, Water, or other Surfaces that
                               are contaminated
                 Foods that
                  could be
                contaminated


USDA NIFSI Food Safety in the Classroom©
 University of Tennessee, Knoxville 2006                     Direct contact
A Closer Look – Where do
                                   you get a pathogen

                                                              Direct
                                                             Contact

                          Indirect
                          Contact


                                           Foods and water
                                               may be
                                            contaminated

USDA NIFSI Food Safety in the Classroom©
 University of Tennessee, Knoxville 2006
Are all bacteria pathogens?
                       • No, most are harmless
                       • Some are even helpful
                                – Examples of helpful bacteria:
                                           • Lactobacillus: makes cheese, yogurt, &
                                             buttermilk and produces vitamins in your
                                             intestine
                                           • Leuconostoc: makes pickles &
                                             sauerkraut
                                           • Pediococcus: makes
                                             pepperoni, salami, & summer sausage

USDA NIFSI Food Safety in the Classroom©
 University of Tennessee, Knoxville 2006
A Closer Look – Helpful
                                     Bacteria                               www.bioweb.usu.edu




                 Pediococcus               - used in
                 production of fermented meats
                                                       Lactobacillus casei – found in
                                                       human intestines and mouth to improve
                                                       digestion




              Leuconostoc cremoris –
             used in the production of buttermilk and
USDA NIFSI Food Safety in the Classroom© sour cream               Lactobacillus bulgaricus –
 University of Tennessee, Knoxville 2006
                                                                  used in the production of yogurt
What are some common
                     pathogens?                                             E. coli
                                                                           O157:H7
                     • Pathogenic E. coli
                       (like O157:H7)
                              – Found in ground beef, contaminated
                                fruits and vegetables
                                                                            Salmonella
                     • Salmonella
                              – Found in raw
                                meats, poultry, eggs, sprouts, fruit and
                                vegetables

                     • Listeria
                              – Found in deli foods, lunch
                                meats, smoked fish and vegetables
USDA NIFSI Food Safety in the Classroom©
 University of Tennessee, Knoxville 2006
                                                                            Listeria
Examples of Pathogens
                          Salmonella
                                                                 E. coli O157:H7




                                           What shape are
              Staphylococcus               these bacteria?
              aureus                        Cocci, bacilli, or
                                               spiral?
                                                                          Campylobacter
                                                                          jejuni


USDA NIFSI Food Safety in the Classroom©
 University of Tennessee, Knoxville 2006
How can I avoid pathogens?
                     • Wash your hands often so you won’t
                       transfer bacteria to your mouth or
                       food
                              – Warm water with soap for 20
                                seconds, rub hard between fingers and
                                nails




USDA NIFSI Food Safety in the Classroom©
 University of Tennessee, Knoxville 2006
How can I avoid pathogens?

                    • Cook food thoroughly to kill
                      any pathogens that may be
                      in your food
                    • Store food properly to limit
                      pathogen growth
                             – Cold temperatures (40 F)


USDA NIFSI Food Safety in the Classroom©
 University of Tennessee, Knoxville 2006
Review
                 • Bacteria are living organisms
                 • Most are harmless
                 • A few are pathogens that make
                   you sick
                 • You can reduce the risk of
                   getting sick by washing your
                   hands and handling food
                   properly.
USDA NIFSI Food Safety in the Classroom©
 University of Tennessee, Knoxville 2006
Stained Bacteria Cells at 4x
Stained Bacteria Cells at 10x
Stained Bacteria Cells at 40x

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Bacteria

  • 1. Introduction to Bacteria USDA NIFSI Food Safety in the Classroom© University of Tennessee, Knoxville 2006
  • 2. What are bacteria? • Single celled organisms E. Coli O157:H7 • Very small can make you very sick. • Need a microscope to see • Can be found on most materials and surfaces Streptococcus – Billions on and in your can cause strep throat. body right now This E. coli helps USDA NIFSI Food Safety in the Classroom© University of Tennessee, Knoxville 2006 you digest food.
  • 3. What do they look like? • Three basic shapes Bacilli – Rod shaped called bacilli (buh-sill-eye) – Round shaped called cocci (cox-eye) Cocci – Spiral shaped • Some exist as single cells, others cluster together USDA NIFSI Food Safety in the Classroom© Cluster of cocci Spiral University of Tennessee, Knoxville 2006
  • 4. Bacteria are ALIVE! • What does it mean to be alive? – They reproduce (make more of themselves) – They need to eat USDA NIFSI Food Safety in the Classroom© University of Tennessee, Knoxville 2006
  • 5. How do bacteria reproduce? • Grow in number not in size – Humans grow in size from child to adult • Make copies of themselves by dividing in half – Human parents create a child USDA NIFSI Food Safety in the Classroom© University of Tennessee, Knoxville 2006
  • 6. How do bacteria eat? • Some make their own food from sunlight—like plants Photosynthetic bacteria • Some are scavengers – Share the environment around them • Example: The bacteria in your stomach are now eating what you ate for breakfast Harmless bacteria • Some are warriors (pathogens) on the stomach lining – They attack other living things • Example: The bacteria on your face can attack skin causing infection and acne E. Coli O157:H7 USDA NIFSI Food Safety in the Classroom© University of Tennessee, Knoxville 2006 is a pathogen
  • 7. What is a pathogen? • Bacteria that make you sick – Why do they make you sick? • To get food they need to survive and reproduce – How do they make you sick? • They produce poisons (toxins) that result in fever, headache, vomiting, and diarrhea and destroy body tissue USDA NIFSI Food Safety in the Classroom© University of Tennessee, Knoxville 2006
  • 8. Where do you get a pathogen? Indirect contact • Contact with people who are sick – Direct or indirect • Food, Water, or other Surfaces that are contaminated Foods that could be contaminated USDA NIFSI Food Safety in the Classroom© University of Tennessee, Knoxville 2006 Direct contact
  • 9. A Closer Look – Where do you get a pathogen Direct Contact Indirect Contact Foods and water may be contaminated USDA NIFSI Food Safety in the Classroom© University of Tennessee, Knoxville 2006
  • 10. Are all bacteria pathogens? • No, most are harmless • Some are even helpful – Examples of helpful bacteria: • Lactobacillus: makes cheese, yogurt, & buttermilk and produces vitamins in your intestine • Leuconostoc: makes pickles & sauerkraut • Pediococcus: makes pepperoni, salami, & summer sausage USDA NIFSI Food Safety in the Classroom© University of Tennessee, Knoxville 2006
  • 11. A Closer Look – Helpful Bacteria www.bioweb.usu.edu Pediococcus - used in production of fermented meats Lactobacillus casei – found in human intestines and mouth to improve digestion Leuconostoc cremoris – used in the production of buttermilk and USDA NIFSI Food Safety in the Classroom© sour cream Lactobacillus bulgaricus – University of Tennessee, Knoxville 2006 used in the production of yogurt
  • 12. What are some common pathogens? E. coli O157:H7 • Pathogenic E. coli (like O157:H7) – Found in ground beef, contaminated fruits and vegetables Salmonella • Salmonella – Found in raw meats, poultry, eggs, sprouts, fruit and vegetables • Listeria – Found in deli foods, lunch meats, smoked fish and vegetables USDA NIFSI Food Safety in the Classroom© University of Tennessee, Knoxville 2006 Listeria
  • 13. Examples of Pathogens Salmonella E. coli O157:H7 What shape are Staphylococcus these bacteria? aureus Cocci, bacilli, or spiral? Campylobacter jejuni USDA NIFSI Food Safety in the Classroom© University of Tennessee, Knoxville 2006
  • 14. How can I avoid pathogens? • Wash your hands often so you won’t transfer bacteria to your mouth or food – Warm water with soap for 20 seconds, rub hard between fingers and nails USDA NIFSI Food Safety in the Classroom© University of Tennessee, Knoxville 2006
  • 15. How can I avoid pathogens? • Cook food thoroughly to kill any pathogens that may be in your food • Store food properly to limit pathogen growth – Cold temperatures (40 F) USDA NIFSI Food Safety in the Classroom© University of Tennessee, Knoxville 2006
  • 16. Review • Bacteria are living organisms • Most are harmless • A few are pathogens that make you sick • You can reduce the risk of getting sick by washing your hands and handling food properly. USDA NIFSI Food Safety in the Classroom© University of Tennessee, Knoxville 2006