Presentation on protein Array
- 1. NEXT-GENERATION HIGH-DENSITY SELF-ASSEMBLING FUNCTIONAL PROTEIN ARRAYS By Arindam Sain
- 2. Serum screening to identify antibody immune responses for cancer Detect post-translational modifications such as phosphorylation Identify protein-protein or protein-DNA interactions High throughput quantification of protein binding kinetics by coupling Protein array with surface plasmon resonance (SPRi) APPLICATIONS
- 4. NEXT GENERATION CELL FREE PROTEIN MICROARRAY Replaces printing proteins with the more reliable and less expensive process of printing DNA Avoids the need to express, purify and store individual proteins Displays 95% of sequence-verified full-length genes Protein display levels are consistent from protein to protein(e.g. differential MW) Assures protein integrity by using both mammalian expression machinery and chaperone proteins to synthesize and fold proteins Easy to create custom arrays
- 5. MAMMALIAN CELL FREE EXPRESSION
- 6. NUCLEIC ACID PROGRAMMABLE PROTEIN ARRAY-NAPPA Plasmid encoding target proteins fused with an affinity tag (e.g. GST) are affixed to surface Array is activated by the addition of a cell free expression system (IVTT mixture) Target proteins are expressed and immobilized in situ, and detected using anti-tag antibody
- 7. WORK FLOW OF NAPPA
- 8. LaBaer et al
- 9. In 2016, cell-free protein microarrays made the jump from bench to bedside with the launch of Provista’s Videssa® test The test, the first blood-based proteomics biomarker assay for early breast cancer detection, includes 28 autoantibody biomarkers that were originally discovered with cell-free protein arrays Videssa® test can significantly reduce the number of unnecessary breast biopsies RECENT ADVANCEMENTS Anderson et al.
- 11. DRUG SCREENING Rauf et al., 2018
- 13. THANK YOU