Scribd
Upload
146 views2 pages

Food Enzymology Exam Paper

This document appears to be an exam for a Food Enzymology course, consisting of 5 questions. Question 1 asks about different types of enzyme regulation, classification of enzymes, and analyzing enzyme inhibition kinetics data. Question 2 asks about examples of different types of enzyme inhibition, regulation of the Trp operon, and deriving equations for enzyme inhibition kinetics. Question 3 asks about drawing enzyme inhibition kinetics plots, explaining several enzyme kinetics terms, and discussing enzyme immobilization. Questions 4 and 5 ask about various topics relating to applications of enzymes such as in food processing.

Uploaded by

feyayel988
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
146 views2 pages

Food Enzymology Exam Paper

This document appears to be an exam for a Food Enzymology course, consisting of 5 questions. Question 1 asks about different types of enzyme regulation, classification of enzymes, and analyzing enzyme inhibition kinetics data. Question 2 asks about examples of different types of enzyme inhibition, regulation of the Trp operon, and deriving equations for enzyme inhibition kinetics. Question 3 asks about drawing enzyme inhibition kinetics plots, explaining several enzyme kinetics terms, and discussing enzyme immobilization. Questions 4 and 5 ask about various topics relating to applications of enzymes such as in food processing.

Uploaded by

feyayel988
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd

Seat No.: ________ Enrolment No.

___________

GUJARAT TECHNOLOGICAL UNIVERSITY


BE - SEMESTER–VII (NEW) EXAMINATION – WINTER 2021
Subject Code:3171410 Date:27/12/2021
Subject Name:Food Enzymology
Tim[Link] AM TO 01:00 PM Total Marks: 70
Instructions:
1. Attempt all questions.
2. Make suitable assumptions wherever necessary.
3. Figures to the right indicate full marks.
4. Simple and non-programmable scientific calculators are allowed.

Q.1 (a) Differentiate between inducible and repressible enzyme regulation. 03


(b) Write a detailed note on classification of enzymes in tabular manner. 04
(c) A chemist measured the initial rate of an enzyme-catalyzed reaction in 07
the absence and presence of inhibitor A and, in a separate procedure,
inhibitor B. In each case, the inhibitor’s concentration was 8.0 mM
(8.0 X 10^-3 M). The following data were obtained:

[S] (M) Vo (M/s) Vo (M/s) Vo (M/s)


No inhibitor Inhibitor A Inhibitor B
5 X 10-4 1.25 X 10-6 5.8 X 10-7 3.8 X 10-7
1 X 10-3 2 X 10-6 1.04 X 10-6 6.3 X 10-7
2.5 X 10-3 3.13 X 10-6 2 X 10-6 1 X 10-6
5 X 10-3 3.85 X 10-6 2.78 X 10-6 1.25 X 10-6
1 X 10-2 4.55 X 10-6 3.57 X 10-6 1.43 X 10-6

Find Vmax for all 3 cases and comment of type of inhibition displayed
by inhibitor A and B. Verify he same using LB equation.

Q.2 (a) Give one example each of competitive, uncompetitive and 03


uncompetitive inhibition.
(b) What is the significance of two consecutive trp codon in regulation of 04
Trp operon
(c) Derive Michaelis–Menten equation and lineweaver burk equation for 07
non-competitive enzyme inhibition.
OR
(c) Derive Michaelis–Menten equation and lineweaver burk equation for 07
uncompetitive enzyme inhibition.

Q.3 (a) Draw both MM and LB plots for competitive, uncompetitive, and non- 03
competitive inhibition.
(b) Explain the following terms: a) Ribozyme b) stereochemical 04
specificity c) induced fit model d)Significance of Km
(c) Explain enzyme immobilization, types, matrices, advantages and 07
disadvantages.
OR
Q.3 (a) Draw a flowchart to quantify the amylase enzyme activity 03

1
(b) Complete the following table in reference to lac operon: 04
Case Repressor Operator CAP/ RNA
occupied site cAMP polymerase
(Y/N) blocked moiety moves
(Y/N) formed forward
(Y/N) (Y/N)
Only glucose
present
Only lactose
present
Both glucose and
lactose present
Both glucose and
lactose absent

(c) Explain the role of enzymes in egg processing or lipid modification. 07

Q.4 (a) Explain regulation of Arabinose operon in case when both inducer and 03
repressor is present
(b) Draw a flowchart to depict purification of chymosin. Give one 04
application of chymosin in dairy industry.
(c) Comment “enzymes play a crucial role in tenderization and flavour 07
development in meat products”.
OR
Q.4 (a) Explain role of enzymes in inactivation of antinutritional factors. 03
(b) Draw a flowchart for MSG production. Suggest few applications of 04
MSG.
(c) Explain enzyme aided flavour extraction from hydrolyzed plant and 07
animal sources.

Q.5 (a) Justify the following: 03


1) Gibbs Energy of [ES] is much higher than S or P.
2) MM plot presented certain practical hurdles.
3) Effect of temperature on enzyme activity is not exactly bell shaped.
(b) Explain the role of enzymes in deskinning and collagen extraction 04
from fishes.
(c) Enlist application of enzymes in baking and brewing industries. 07
OR
Q.5 (a) Comment on application of immobilized enzymes. 03
(b) Mention the enzymes used for liquefaction, saccharification, and 04
dextrinization, isomerization of starch.
(c) What are the applications of enzymes in dairy industry? 07

*************

You might also like