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Preparing Haemochromogen Crystals

The document describes a method for preparing haemochromogen crystals using human blood. The method involves using Takayama's reagent, which is a mixture of sodium hydroxide, pyridine and glucose solution. When a drop of human blood is added to the reagent on a microscope slide, it forms characteristic red crystals called haemochromogen crystals. These crystals allow for the microscopic examination and identification of haemoglobin in blood. The formation of the crystals involves alkaline hydrolysis and oxidation of haemoglobin in blood.

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2K views13 pages

Preparing Haemochromogen Crystals

The document describes a method for preparing haemochromogen crystals using human blood. The method involves using Takayama's reagent, which is a mixture of sodium hydroxide, pyridine and glucose solution. When a drop of human blood is added to the reagent on a microscope slide, it forms characteristic red crystals called haemochromogen crystals. These crystals allow for the microscopic examination and identification of haemoglobin in blood. The formation of the crystals involves alkaline hydrolysis and oxidation of haemoglobin in blood.

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aashiwrites09
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Preparation of haemochromogen crystals using human blood

Class: [Link]. Life Sciences (2nd Year, 3rd Sem)


Paper: Physiology and Biochemistry

Presented by
Dr. Jitendra K. Chaudhary; PhD

Disclaimer: The abovementioned teacher and/or students, hereby, make no claims over the any part of the content, including figure,
diagrams, scheme or any other original picture, to be his/her own, rather would like to duly acknowledge the original authors
referenced therein at the end whose contents are compiled in this presentation, solely for the purpose of teaching and discussion as
part of the aforementioned programme of study. Therefore, any person, in possession of this content, must not reproduce, publish or
use in any other academically dishonest way, any part of it without due acknowledgment to the original authors referenced. In
addition, presenter would like to be excused for inadvertent failure to acknowledge any other authors or original content developer (s),
if left duly unacknowledged.
Formation of blood cells
Materials: Spirit lamp, dropper, cotton, match box, light microscope, pricking needle, cover slips, glass slides.

Chemicals/Reagents: Human blood, Takayama’s Reagent (10% NaOH-3 ml; Pyridine-3 ml,
Saturated glucose solution-3 ml; DW-7 ml).
Formation of Takayama (Hemochromogen) Crystals

2+

Pyridine Pyridine
References

1. Gayatri Prakash (2012). Lab Manual on Blood Analysis and Medical Diagnostics. First Ed. S. Chand &
Company Pvt. Ltd.

2. Henry C. Lee (1978). Identification and Grouping of Bloodstains, University of New Haven (pp 267-277).

3. Wikipedia: [Link]
EPsO63qAE&q=Haemoglobin&oq=Haemoglobin&gs_lcp=CgZwc3ktYWIQAzIFCAAQsQMyBQgAELEDMggIABCxAxCDATIFCAAQsQMyAggAMgIIADIFCAAQsQMyAggAMg
UIABCxAzICCAA6BwgjEOoCECc6BwguEOoCECc6BAgjECc6BAgAEEM6BwgAELEDEEM6BQguELEDOgQIABAKUNaPAViLrwFg57EBaAJwAHgBgAG6CIgB0iGSAQsyLTQuN
C42LTEuMZgBAKABAaoBB2d3cy13aXqwAQo&sclient=psy-ab&ved=0ahUKEwjpr_Pz2brrAhV1xzgGHTD3DRUQ4dUDCAc&uact=5

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