BD Vacutainer® System
BD Diagnostics, Preanalytical Systems
Tube Guide including Order of Draw
Please display this in clinical areas beside your venepuncture equipment
HDFT Pathology (Nov 2008 update) - ALWAYS USE ICE REQUEST FORMS
For futher advice (eg Paed tubes) see the Pathology Handbook or tel. 01423 55(3000)
Blood Samples should be taken in the following order:
Catalogue Colour Code Tube Type Determinations Special Instructions
Number
Blood Aerobic followed by anaerobic - 8-10mls per bottle. If insufficient blood for both culture bottles,
Draw 8-10 Culture use aerobic bottle only. Wipe rubber septum with fresh alcohol steret before use.
Volume ml
363093 Coagulation Screen (PT and APTT)
INR, & Fibrinogen
Soduim Thrombophilia / Haemophilia samples: Mon-Thurs
Draw Thrombophilia (x4) or (x2) 4.5 mls +
Citrate only
Volume 1.8ml 1xGold +1xEDTA
Light Blue
Haemophilia Screen (x2)
Sodium
NOT IN USE AT THIS HOSPITAL
Draw Citrate
Volume Black
IMPORTANT: Both must be kept at 37 Degrees C
367837 Serum / Cold Agglutinnins - special instructions,
Cold Aggs by prior arrangement only - contact lab on
Draw Plastc Cryoglobulins - special instructions
3069
Volume 6ml Red
All Routine Chemical Pathology Tests, incl
367954 hormone & cortisol-ethanol
SST™ II Ethanol: inpatients only
Draw Paul Bunnell (IM), Vit B12 & Folate,
Volume
5ml Immunology tests, Microbiology Serology
Gold
Lithium
367375 Heparin Renin, Ammonia, Chromosome, BNP &
NB: BNP & Troponin have separate ICE request forms
Draw and Troponin plus other special assays
Volume 4.5mlGreen PST™ II
FBC, Plasma Viscosity, Hb A1C, G6PD, PTH, PV: Seperate tube required (eg FBC+PV = 2 x EDTA)
368860 Lead, DAGT (DCT), Mercury, Hb Ammonia: contact 3000 for collection instructions
EDTA
Draw Elctrophoresis, HLA B27 (x2), Ammonia, HLA B27: 4 Points of ID (Full name, DoB, Hospital or
Volume
4ml Molecular (DNA) investigations NHS Number and Address) - Handwritten please
Lavender
Cross
367941 Blood Group & Save Serum, Crossmatch, All transfusion samples MUST be clearly handwritten
Match
Draw EDTA Antenatal serology & include Full Name, DoB, Sex and Hospital number
Volume
6ml
Pink
367692 Fluoride Fasting Glucose, Non-fasting Glucose,
Lactate: Needed in Laboratory ASAP
Draw Oxalate Lactate
Volume
2ml
Grey
367737 Trace
Zinc
Draw Element
Volume
7ml
Royal Blue
RECOMMENDED ORDER OF DRAW: BD Diagnostics, Preanalytical Systems
1. Tubes with no Additive Between Towns Road, Cowley, Oxford. OX4 3LY
2. Coagulation Tubes Tel. No.: 01865 781540 Fax: 01865 781528
3. Other tubes with ADDITIVES BD, logo, Vacutainer, Hemogard are all trademarks of Becton Dickinson & Company
BD Vacutainer System ®
BD Diagnostics - Preanalytical Systems
Mixing Guidelines
All BD Vacutainer tubes require immediate mixing following collection
®
Colour Code Tube Type Inversions
Sodium Citrate 3-4 Times
Light Blue
Serum 5-6 Times
Red
SST II
TM
5-6 Times
Gold
Heparin & PST IITM
8-10 Times
Green
EDTA 8-10 Times
Lavender
Cross Match 8-10 Times
Pink
Fluoride Oxalate 8-10 Times
Grey
Trace Element 8-10 Times
Royal Blue
Insufficient mixing can result in inaccurate test results and the need to re-draw
