1
LAKSHYA (NEET)
Biotechnology: Principles and Processes – [DPP-02]
1. In recombinant DNA technique, the term 5. Presence of more than one recognition site
cloning vector refers to a within vector will lead to
(A) donor DNA, it is identified and picked (A) generation of several fragments
up through electrophoresis
(B) generations of one fragment
(B) plasmid, transfers DNA into living cell
(C) generation of half fragment
(C) collection of entire genome in the form
(D) None of the above
of plasmid
6. The recognition site for BamH I in pBR322
(D) enzyme, cuts the DNA at specific sites
is present in
2. During ‘gene cloning’ which is called a
(A) ampicillin resistant site
gene taxi?
(B) tetracycline resistant site
(A) vaccine (B) Plasmid
(C) ori site
(C) Bacteria (D) Protozoa
(D) rop site
3. Which of the following is a plasmid vector?
7. Which of the following in not a feature of
(A) pBR322 (B) BamHI
the plasmids?
(C) Sal I (D) EcoRI
(A) Circular structure
4. Identify A, B, C and D in the given
(B) Transferable
diagram of cloning vector pBR322.
(C) Single-stranded
A
(D) Independent replication
8. When an alien DNA is ligated in
B tetracycline resistant gene, the recombinant
D will
(A) become tetracycline resistance
(B) will loose tetracycline resistance
C (C) will remain same
(D) None of the above
9. The method(s) that is/are used to
differentiate recombinant and non-
recombinants is/are
(A) A-EcoR I, B-BamH I, C-Ori, D-ampR
(A) antibiotic resistant gene
(B) A-ampR, B-Ori, C-BamH I, D-EcoR I
(B) insertional inactivation
(C) A-Ori, B-BamH I, C-EcoR I, D-ampR
(C) gene cloning
(D) A-BamH I, B-EcoR I, C-ampR, D-Ori
(D) Both (A) and (B)
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10. In insertional inactivation in presence of 15. Which of the following statement is not
chromogenic substrate, a recombinant correct regarding EcoRI?
DNA is inserted within the coding
I. EcoRI restriction endonuclease enzyme
sequence of
is isolated from Escherichia coli RY13.
(A) β − galactosidase
II. Its recognition sequence is
(B) tetracycline resistant gene 5’- GAATTC- 3’
(C) restriction enzyme
3’-CTTAAG-5’
(D) ampicillin resistant gene
III. Its site of cleavage is
11. Recombinant colonies in insertional
inactivation are differentiated on the basis 5’-G↓-A-A-T-T-C-3’
of 3’-C-T-T-A-A-↑G-5’
(A) produce blue color (A) I and II (B) I and III
(B) show no colour
(C) I, II and III (D) None of these
(C) produce red colour
16. There is a restriction endonuclease called
(D) no change EcoRI. What does ‘co’ part in it stands for?
12. The function of ori in a vector is
(A) Coelom
(A) help in replication of linked DNA
(B) Strain of bacterium
(B) control copy number of the linked
DNA (C) coli
(C) help in selecting recombinants (D) Colon
(D) Both (A) and (B) 17. The roman number following the name of
13. Which of the following is used in restriction enzyme indicate
recombinant DNA technique? (A) order in which enzyme is isolated from
(A) Cell wall of virus strain of bacteria
(B) Gene which produces capsid of virus (B) number of enzyme
(C) Virus (C) order of enzyme
(D) Capsid of virus
(D) None of the above
14. Consider the following statements.
18. Which of the following is a restriction
I. Recombinant DNA technology endonuclease?
popularly known as genetic
engineering which deals with the (A) Protease (B) DNase I
manipulation of genetic material by (C) RNase (D) Hind II
man in vitro.
19. The mode of action of exonucleases is
II. pBR322 is developed by Boliver and
Rodriquez from E. coli plasmid. (A) remove nucleotides from specific place
III. Restriction enzymes belongs to a class (B) remove nucleotides from the ends of
of enzymes called nucleases. DNA
Which of the statements given above are (C) remove nucleotides in between the
correct? DNA
(A) I and II (B) I and III (D) remove nucleotides
(C) II and III (D) I, II and III
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20. In the naming of restriction enzymes the 23. Plasmids are extra-chromosomal genetic
first letter is derived from ….A…. and material found in
next two letters from the ….B… and fourth
(A) Algae
letter from ….C… of ...D…. where the
enzymes are extracted. (B) Mammalian cell
A to D is the statement can be (C) Bacteria
A B C D (D) Viruses
(A) genus species strain bacteria 24. Agrobacterium tumefaciens deliver a piece
of DNA into a dicot plant. The piece of
(B) species genus strain bacteria
DNA is called as
(C) genus species variety eukaryote
(A) rDNA (B) T-DNA
(D) species genus variety eukaryote
(C) mDNA (D) cDNA
21. A foreign DNA and plasmid cut by the
25. Retroviruses in animals including humans
same restriction endonuclease can be joined
are able to change normal cells into
to form a recombinant plasmid using
(A) germ cell
(A) EcoRI
(B) cancerous cells
(B) Taq polymerase
(C) plasmid
(C) Helicase
(D) vector
(D) ligase
22. If gene of interest was inserted at Pst I site
in pBR322 the resulting plasmid will
confer resistance to
(A) Ampicillin
(B) Tetracycline
(C) Kanamycin
(D) Both A and C
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ANSWERS
1. (B)
2. (B)
3. (A)
4. (A)
5. (A)
6. (B)
7. (C)
8. (B)
9. (D)
10. (A)
11. (B)
12. (D)
13. (C)
14. (D)
15. (D)
16. (C)
17. (A)
18. (D)
19. (B)
20. (A)
21. (D)
22. (B)
23. (C)
24. (B)
25. (B)
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