T720 SERIES

OPERATOR MANUAL
(Please Read This Manual Before Using the Microscope)

T720 Series
Compound Microscope
T720 Series Microscope

Copyright © 2015 United Scope Inc. DBA AmScope. All rights reserved.

Copyright Notice

This document, which is in draft form and subject to change without notice, is an
unpublished work containing confidential, trade secret information that is proprietary to
AmScope, and is protected by laws pertaining to such materials. This document, the
information in this document and all rights thereto are the sole and exclusive property of
AmScope and are not to be copied, used or disclosed to anyone, in whole or in part,
without the express written permission of AmScope.

AmScope assumes no liability for any damages incurred, directly or indirectly, from any
errors, omissions or discrepancies between the use of this microscope and the information
contained in this document.

AmScope
Purchasing: 949-333-0001 or 1-888-950-2888 FREE (toll free)
Customer Service: 949-333-0002
Technical Support: 949-333-0004
Shipping: 949-333-0005
Accounting: 949-333-0008

Fax: 949-333-0007

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Contents
BEFORE USE..............................................................................................................................................................4
Introduction .........................................................................................................................................................4
Precautions ..........................................................................................................................................................4
1. MICROSCOPE PARTS ...............................................................................................................................................5
2. OPERATION ............................................................................................................................................................7
2.1 Unpacking ......................................................................................................................................................7
2.2 Assembly ........................................................................................................................................................8
2.3 Adjusting the View ........................................................................................................................................11
2.4 Specimen Set Up...........................................................................................................................................12
2.5 Focusing.......................................................................................................................................................13
2.6 Using the Trinocular Port .............................................................................................................................14
2.7 Attaching a Camera......................................................................................................................................14
2.8 Setting the Stage’s Stop-Limit........................................................................................................................15
2.9 Adjusting Focus Tension...............................................................................................................................15
2.10 Setting the Condenser Lens Adjustment Knob ..............................................................................................15
2.11 Adjusting the Iris ........................................................................................................................................16
2.12 Changing a Filter (Optional Accessory) ......................................................................................................16
2.13 Changing the Fuse ......................................................................................................................................16
2.14 Using Oil Immersion...................................................................................................................................17
2.15 Using Darkfield Condenser Lenses (Optional Accessories)..........................................................................19
2.16 Using a Dry Darkfield Condenser Lens (Optional Accessory)......................................................................20
2.17 Using Oil Darkfield Condenser Lens (Optional Accessory)..........................................................................21
2.18 Microscope Maintenance ............................................................................................................................24
2.19 Darkfield Condenser Kit Maintenance.........................................................................................................25
3. GENERAL SPECIFICATIONS ...................................................................................................................................26
3.1 Features .......................................................................................................................................................26
3.2 Eyepiece Comparison ...................................................................................................................................26
3.3 Eyepiece Parameters ....................................................................................................................................26
3.4 Objectives.....................................................................................................................................................27
3.5 Darkfield Condensers ...................................................................................................................................27
4. PARAMETERS .......................................................................................................................................................28
4.1 Electrical System ..........................................................................................................................................28
4.2 Microscope Parameters ................................................................................................................................28
5. RECOMMENDED ACCESSORIES..............................................................................................................................29
5.1 Eyepieces......................................................................................................................................................29
5.2 Darkfield Condensers ...................................................................................................................................29
5.3 Objective Lenses...........................................................................................................................................30
5.4 Fuses............................................................................................................................................................30
5.5 Cameras and Accessories..............................................................................................................................31
5.6 Slides............................................................................................................................................................32
5.7 Cleaners .......................................................................................................................................................32
5.8 Books ...........................................................................................................................................................32
6. TROUBLESHOOTING .............................................................................................................................................33
6.1 Optical Issues ...............................................................................................................................................33
6.2 Mechanical Issues.........................................................................................................................................34
6.3 Electrical Issues............................................................................................................................................35
7. GENERAL MICROSCOPY GUIDE .............................................................................................................................36

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T720 Series Microscope

Before Use
Introduction
Congratulations on the purchase of your new AmScope Microscope! This manual is
designed for T720 series microscopes. Though the microscope’s features may vary
depending on your series model number, the T720 series functions remain the
same.

Please take a few minutes to familiarize yourself with the features and functions of
your new microscope. If you want more information on microscope, parts, and
accessories, please visit our website at:

www.AmScope.com

We recommend that you study this manual thoroughly before operating the
microscope and that you keep it on hand for future reference. If you have additional
questions or need assistance, please send us an email at:
[email protected]
Please include the microscope model number in your email so that we can identify
your model and provide immediate help.

Precautions
1. As the microscope is a precision instrument, always handle it with care, avoiding
impact or abrupt movement during transportation. Do not shake the package.
2. Do not place the microscope in direct sunlight or in high heat. Keep it indoors in a
dry and clean place with temperatures between 32°-100° F (0°-40°C), maximum
relative humidity: 85%
3. Avoid touching the lenses on the objective and the eyepiece so that oil and dirt
from your fingerprints do not obstruct your view.
4. Before turning the power on, make sure that the power supply voltage is
consistent with the voltage of your microscope.

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1. Microscope Parts
1.1 Parts Diagram

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1.2 Features
This brand new professional trinocular compound microscope comes equipped with
an infinity plan optical system and offers advanced features as well as research grade
performance. Its crystal clear optical and precise mechanical systems promise high
quality performances for advanced applications. The backward nosepiece design
allows spacious room for convenient operation.

This microscope has five high quality Plan Achromatic objectives (4X, 10X, 20X, 40X,
100X), depending on the model one or two pair of extreme widefield eyepieces, a
Kohler illumination system, a 30-degree incline on the ocular tubes for comfortable
viewing, 360-degree swiveling, compensation-free trinocular head, a coaxial coarse &
fine focusing system, and a 3D mechanical stage. It is an ideal instrument for
advanced research.

Ø Infinity Corrected Optical System with High Resolution

Ø Fully Coated Optics with Crystal Clear & Sharp Images
Ø Precise Mechanical Control System
Ø Reversed Nosepiece Design
Ø Kohler Illumination System with Field Diaphragm for Lighting Control
Ø 30-Degree Inclined, 360-Degree Swiveling, Compensation Free Trinocular
Head
Ø Five Magnification Levels: 40X, 100X, 200X, 400X, 1000X (for the T720, T720-
TP & T720-HD18 models)
Ø Eight Magnification Levels: 40X, 100X, 200X, 250X, 400X, 500X, 1000X &
2500X (for the T720QC, T720QC-TP & T720QC-HD18 models)
Ø Intensity-Variable Transmitted LED Lighting System
Ø Abbe Condenser with Iris Diaphragm and Swing-Out Filter Holder
Ø Rack and Pinion Adjustment for Condenser
Ø Low Position Coaxial Stage Movement Controlling Knobs
Ø Dual Side Coaxial Coarse and Fine Focusing Control
Ø Adjustable Interpupillary Distance
Ø Adjustable Diopter on Eyepieces
Ø Durable Cast Alloy Frame with Stain Resistant Enamel Finish
Ø Five Infinity Plan Objectives Included
Ø One Pair of 10X Extreme Widefield Eyepieces Included
Ø One Pair of 25X Extreme Widefield Eyepieces Included (for the T720QC,
T720QC-TP & T720QC-HD18 models only)
Ø Quintuple, Reversed, Extra-Large Nosepiece with Wide, Knurled Grip for Easy
Operation
Ø Large Double Layer Mechanical Stage with Stain Resistant Coating
Ø Upward Stage Limit Stop to Protect Objectives and Slides
Ø Manufactured under ISO 9001 Quality Control Standards
Ø Excellent Five (5) Year Factory Warranty

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2. Operation
2.1 Unpacking
2.1.1 Unpack the Box
1. Very carefully slide the Styrofoam container out of the cardboard carton.
2. Lay the Styrofoam container on its side. Make sure the side labeled up is up.
3. Remove the tape.
4. Carefully open the Styrofoam container, avoid dropping and damaging the
optical items.

2.1.2 Verify Packing List

Check the packing list to ensure that you’re received all items:
Ø Trinocular Compensation-Free Head
Ø Microscope Body with Frame, Base, and Kohler LED Illumination System
Ø Five High Quality DIN Plan Achromatic Objectives: 4X, 10X, 20X, 40X and
100X
Ø One Pair of Plan Extreme Widefield PL10X Eyepieces
Ø One Pair of Extreme Widefield 25X Eyepieces (for the T720QC, T720QC-TP
& T720QC-HD18 models only)
Ø 1 Unique 9.7" true color LCD screen and 5MP camera system (for the
T720QC-TP model only)
Ø 1920x1080p HDMI Camera (for the T720-HD18 & T720QC-HD18 models
only)
Ø TC2 Camera Body (for the T720-HD18 & T720QC-HD18 models only)
Ø HDMI Cable (for the T720-HD18 & T720QC-HD18 models only)
Ø Mounting Adapter (for the T720-HD18 & T720QC-HD18 models only)
Ø 90V-240 Wide Voltage Power Supply
Ø Dust Cover
Ø Immersion Oil

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T720 Series Microscope

2.2 Assembly
2.2.1 Carefully Remove Microscope from Box

Remove the microscope body from the box and

remove the plastic protective covering.

The body of the microscope is composed of the base,

the stage, the arm, and the nosepiece.

2.2.2 Loosen the Head Lock Screw

Loosen the metal knob located directly above the

nosepiece (the head lock screw) completely.

2.2.3 Install the Microscope Head

Carefully take the microscope head out of the plastic bag, making sure not to
touch glass.

Place the bottom side of the microscope head (flat, circular side down) into
the circular opening on top of the arm (just above the nosepiece and lock
screw).

Tighten the head lock screw to secure the head in

place.

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2.2.4 Install the Trinocular Port and C-Mount

Unscrew and remove the photo port cap from the top side of
the microscope head.

Screw in the Trinocular port.

If your camera has a c-mount, insert the c-mount adapter

onto the Trinocular port. If your camera does not have a c-
mount, you can skip this step.

2.2.5 Insert Eyepieces

Remove the eyetube caps.

Place the desired eyepieces into the empty

eye/ocular tubes.

Be sure to avoid touching the lenses.

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2.2.6 Insert Objective Lenses

Unpack the objective lenses. Screw the objectives into the microscope nosepiece.

Start with the lowest magnification, and in order

of magnification, insert each lens, without
touching the glass.

2.2.7 Plug it in, turn it on, and adjust the dimmer if more light is needed.

Plug in the microscope and turn it on.

If no light emerges from the light source, adjust

the dimmer knob on the side of the base.

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2.3 Adjusting the View

2.3.1 Choose forward or reverse viewing

The microscope head rotates to allow for forward or reverse viewing.

There is no difference as far as the optics; it depends on what’s comfortable. In

forward position you are looking over the stage, in reverse position you are
looking at the stage.

Once you’ve chosen your preferred microscope head position, lock the head-
lock.

2.3.2 Focus the distance between your eyes.

(The interpupillary distance, the distance between your eye pupils.)

With both eyes open, look into the eyepieces. Adjust the interpupillary distance
by holding the eyetubes and rotating the tubes either towards or away from each
other until only one circle of light is seen by both eyes.

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2.4 Specimen Set Up

2.4.1 Prepare the Specimen

Place the specimen to be studied on a glass slide

(or use a prepared slide, purchase separately).

2.4.2 Secure the Slide

Place the slide on the stage, holding it snugly in

place with the metal slide holders (clips) of the
mechanical stage.

2.4.3 Center the Specimen

Using the mechanical stage controls, center the

slide over the stage opening, lining it up with the
light and the objective lens.

The top ring moves the stage forward and

backward. The bottom ring moves the stage from
left to right.

2.4.4 Adjust the dimmer if more light is needed.

To adjust the illumination, slowly turn the dimmer on

the right side of the base until the desired intensity
of light is achieved.

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2.5 Focusing
2.5.1 Choose an Objective Lens
Turn the nosepiece to choose an objective. It is easiest to
use the lowest magnification first (4X objective) to locate
and focus on the specimen. As you move up in
magnification you will need to refocus the image a little
each time.

2.5.2 Parfocal

Under the diopters on the eyepieces, there are numbered

rings. Set these rings to 0 and the image should remain
in focus for the entire magnification range. This will
enable you to stay more in focus when you add a camera
or switch objective lenses.

2.5.3 Focus one eye at time

1. Look with one eye through the right eyetube.

2. Close your other eye.
3. Focus the image using the coarse focusing knob.
Adjust the height of the stage until the sample
comes into clear focus.

Note: You can loosen the limit-stop knob (located on the inside of the coarse
focusing knob on the left side of the microscope) in order to give yourself the
full range of motion.

2.5.4 Focus your other eye

1. Close the first eye. Open the other one.

2. Look with the second eye through the eyepiece.
3. Turn the diopter until the image is clear through
the side as well.

2.5.5 Re-center Specimen and Re-focus

When you change magnification levels, you may need to re-center the specimen.
When you change magnification, you will need to re-focus.

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T720 Series Microscope

2.6 Using the Trinocular Port

The AmScope T720 model is uniquely designed so that you can view the image
through the eyepieces and the Trinocular port simultaneously, as well as fine
tune the focus of the camera with a C-mount focus adjustment.

You do not need an adapter to attach your AmScope camera to the Trinocular
port; however you may need one if you have a non-AmScope camera. Our photo
port is a 23mm size.

2.7 Attaching a Camera

Most AmScope cameras come with a reduction lens.

Reduction lenses align the magnification of the
microscope’s image with the capacity of the digital
camera’s sensor.
The specimen’s image seen through the magnified
objective lens is very large. The reduction lens renders
the image in a size compatible with the smaller size of
the digital camera’s sensor.

1. If your camera has a 23mm mounting size, you do

not need the C-mount adapter. If installed, remove
the C-mount from the top of the Trinocular port by
loosening the screw.
2. Place the camera (mounted on a reduction lens)
directly into the Trinocular port. It should slide in
without issue.
3. Focus through the Trinocular port, by turning the
middle portion of the tube.
4. Open up your AmScope Digital Camera Solution
software on your computer and let it connect to your
microscope.

If your camera needs a C-mount adapter, attach the adapter

to your camera and screw the adapter into the Trinocular
port. Plug one end of the cord directly into the camera and
the USB end into your computer.

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2.8 Setting the Stage’s Stop-Limit

1. Unlock the stop-limit on the stage.

2. Adjust the stage to the desired maximum height.
3. Lock the stop-limit. This allows you to limit the movement of the stage from
the bottom of the range up to the point it is set at.
4. To reset it, unlock the stage and reset the stage to the new height.
If no limit is desired, simply unlock the stop-limit.

2.9 Adjusting Focus Tension

1. To adjust the tension of the focusing knobs,

first locate the black ridged tension knob on
the inside of the coarse focusing knob.
2. To decrease tension, rotate the adjustment
forward, towards the stage
(counterclockwise). To increase, rotate away
from the stage (clockwise).

Note: If your stage is slipping down after setting the focus, you need to increase the
tension.

2.10 Setting the Condenser Lens Adjustment Knob

The condenser adjustment knob raises and

lowers the condenser’s distance to the base
lens, varying light delivery.

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2.11 Adjusting the Iris

By changing the aperture (hole size) of the iris/diaphragm

of the condenser, you can adjust the background
brightness. Adjust the aperture of the iris diaphragm
using the numbered iris adjustment ring located directly
under the stage.

2.12 Changing a Filter (Optional Accessory)

1. At the bottom of the condenser lens, there is a small ring that slides out.
Swivel the ring as far out as possible.
2. Insert the preferred colored glass filter.
3. Slide the ring back into place.

Note: The filter holder is placed in from the factory in a manner in which it swings out
and hits the arm of the microscope (backwards). If this happens, simply grip the
condenser assembly and rotate it. It may take a small amount of force to rotate it,
but after doing so, you will be able to swing the filter holder out towards the front
of the unit for easier operation.

2.13 Changing the Fuse

The fuse port is on the back of the microscope on the base between the on/off
switch and the power inlet.

1. Turn off and unplug the microscope.

2. Be sure to remove the eyepieces from the unit
before turning the microscope over to prevent
them from falling and breaking.
3. Carefully turn the microscope on its side,
exposing the base.
4. Using a flat head screwdriver, unscrew the
cover and replace the T1A fuse.
5. Screw the fuse cover back in.

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2.14 Using Oil Immersion

When using the 100X objective, a drop of immersion oil should be

placed between the cover slip and the objective to minimize distortion
caused by air.

1. Place the slide on the stage and center the

specimen.

2. Close the condenser’s aperture iris and ensure that the

specimen focused and centered as possible. Increase
light intensity once the specimen is clearly illuminated.

3. Move the objectives out of the way to enable access

to the slide. Place a drop of immersion oil directly
onto the slide’s cover slip, not directly on the
specimen; a cover slip MUST be used.

4. Release the stage stop limit.

5. Lower your oil immersion lens (100X objective) into

position so that the front lens is immersed in the oil. If
your lens is not immersed, use the coarse focusing
knobs to adjust the height of the stage to the proper
levels. The oil lens should be immersed in oil, but not
touching the slide.

6. Adjust the focusing knob, watch for the field of

view to come into focus. This is best seen when
the iris of the condenser is smaller than the field of
the objective. Use the fine focusing knob to make
adjustments.

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T720 Series Microscope

7. Re-engage the stage stop limit.

8. When the specimen is in focus, adjust the iris to

open just larger than the condenser’s diameter.

9. After using the oil immersion lens, you will need to clean the lens and
specimen before the oil dries.

Note: Please be careful when using the stage stop limit. The stage limit stop
is designed to prevent impact between objective and slide, so when it is off
you can damage the microscope or the slide.

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 T720 Series Microscope

2.15 Using Darkfield Condenser Lenses (Optional Accessories)

Darkfield Illumination is a lighting method for increasing contrast. A darkfield
condenser lens produces bright images against a dark background for low contrast
biological specimens.

The dry darkfield condenser is for low power magnifications,

such as with the 4X, 10X or 40X objective lenses, with
numeric apertures of .65 or less.

The oil darkfield condenser is for high power magnifications,

the 40X and 100X objective lenses, with numeric apertures
of 1.25 or higher.

2.15.1 Installing a Darkfield Condenser Lens

1. Use the condenser lens adjustment knob to
lower the condenser so you have easy access to
the condenser knob.
2. Loosen the condenser lock screw and replace
the condenser lens.
3. Install the darkfield condenser lens and tighten
the condenser lock thumb screw on the
condenser holder.

When choosing a darkfield condenser, be sure the

condenser’s numerical aperture is larger than the
numerical aperture of the objective lens.

If the objective lens aperture is larger than the aperture of

the darkfield condenser, direct light will be transmitted and
a darkfield will not be achieved.

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2.16 Using a Dry Darkfield Condenser Lens (Optional Accessory)

2.16.1 Using the Dry Darkfield Condenser

1. The first step is centering the condenser. Move the 10X objective lens into the
light path.

2. Remove an eyepiece and look through the empty

eye tube, to see the condenser’s image directly.

3. Use the condenser lens adjustment

knob to adjust the height of the
condenser until the opaque disc is
slightly smaller than the entire image
circle, leaving a ring of light.

4. Turn the condenser’s centering screws to move the

dark spot to the center.

5. Replace the eyepieces.

6. Raise the condenser to the top of its range, just short

of the slide on the stage. The is the optimal position
for darkfield viewing, but can be adjusted later, if necessary.

7. Place a slide on the stage and focus. Adjust

the height of the stage up or down slightly for
optimal viewing, but the condenser lens
should be relatively close to the slide.

8. If you are using a variable numerical

aperture objective lens, adjust the ring at
the bottom to fine tune the darkfield.

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2.17 Using Oil Darkfield Condenser Lens (Optional Accessory)

The condenser works with 100X oil objective and low power objectives (4X, 10X,
etc) as well. Immersion oil is needed on top of the condenser lens for darkfield
observation. Immersion oil is needed between the 100X oil objective and slide.

2.17.1 Using the Oil Darkfield Condenser

1. The first step is centering the condenser. Move the 40X objective lens into the
light path.

2. Remove an eyepiece and look through the empty

eye tube, to see the condenser’s image directly.

3. Use the condenser lens adjustment

knob to adjust the height of the
condenser until the opaque disc is
slightly smaller than the entire image
circle, leaving a ring of light.

Note: The dark spot has a fuzzy edge and will become larger when the condenser
gets closers to the objective. The distance between the top of the condenser
and the objective is about 8mm, when the dark spot shows in the field of view.

4. Turn the condenser’s centering screws to move the

dark spot to the center.

5. Replace the eyepieces.

6. Raise the condenser to the top of its

range, just short of the slide on the stage.
The is the optimal position for darkfield
viewing, but can be adjusted later, if
necessary.

7. Adjust the dimmer so the lamp is at maximum brightness.

8. Place a slide on the stage and focus. Adjust the height of the stage up or
down slightly for optimal viewing, but the condenser lens should be relatively
close to the slide.

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2.17.2 Using Oil Immersion

1. Before you install the oil darkfield condenser lens,

apply a drop of immersion oil on the top lens of
condenser. If you’ve already installed it, remove
the condenser to apply the drop of oil to the lens,
and re-install it. You might need to re-center the
condenser.

2. Place the slide on the stage.

3. Raise the condenser and let the oil drop contact

the underside of the slide. If air bubbles exist in
the oil, clean the oil from the condenser lens and
bottom of slide and repeat the procedures.

4. Apply a drop of immersion oil to the slide’s

coverslip.

5. Carefully raise the stage until the oil makes

contact with the objective lens.

6. Adjust the iris ring to get proper brightness and

contrast of view field.

7. If you are using a variable numerical aperture

objective lens, adjust the ring at the bottom to
fine tune the darkfield.

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2.17.3 Cleaning the Oil Darkfield Condenser

1. When using oil immersion, once you have finished using the oil darkfield lens,
you will need to clean the lens and specimen of the oil before it dries.
2. You can wipe the lens with a soft nonabrasive cloth to remove the oil. If you
want to use a cleaner, you can use cigarette lighter fluid to safely remove the
oil, as it will break it down. Do not use alcohol based solvents, as they
dissolve the cement used to in assembly of the lens (thus breaking your
objective).
3. The specimen itself can be discarded, or if desired, you could clean it up and
store it for later use. The specimen may also be left with the oil on it, as you
will still need to apply more for use later, however it may dry up and cloud
your specimen. Cleaning is recommended.

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2.18 Microscope Maintenance

2.18.1 Handle with Care

As the microscope and all accessories are precision instruments, always handle
them with care, avoiding impact or abrupt movement during transportation. Do not
shake the package.

2.18.2 No Sun/Heat
Do not place the microscope and accessories in direct sunlight or in
high heat.
Keep the microscope and all accessories indoors in a dry and clean
place with temperatures between 32°-100° F (0°-40°C), maximum
relative humidity: 85%.
2.18.3 No Dust
Always keep the microscope covered by the dust cover when not in
use. The glass will attract dust, obscuring your view. Make sure to
keep it in a dry and clean place in order to prevent rust.

2.18.4 Keep Glass Clean

All glass surfaces must always be kept clean.
Oil and dirt from your fingerprints will obstruct your view.
Fine dust on the optical surface should be blown off using a hand blower or
gently wiped off with a soft lens tissue.
2.18.5 Use an optical lens cleaner.

Carefully wipe off oil or fingerprints on lens surfaces using a tissue moistened with
a small amount of 3:7 alcohol to ether mixture.

2.18.6 Do not use lens cleaner on the non-glass parts of the microscope.
Do not use the optical lens cleaning solution to wipe the surfaces of the other
components of the microscope.
All other parts, especially those made of plastic, should be cleaned with a mild
detergent.
2.18.7 Do not touch the glass.

Avoid touching the lenses on the objective, the eyepiece and all the
accessories with glass lenses.

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2.18.8 Do not take apart the microscope.

Do not assemble or disassemble the microscope’s electrical

components yourself.
.

2.19 Darkfield Condenser Kit Maintenance

2.19.1 Secure Setting

Ensure that the microscope is located on a smooth, level and firm
surface.

2.19.2 Do Not Take Apart

Do not attempt to disassemble any components.

2.19.3 Keep Kits Clean

Keep the kits clean; remove dirt and debris regularly.
Accumulated dirt on outer surfaces should be cleaned with a
damp cloth. More persistent dirt should be removed using a mild
soap solution. Do not use organic solvents for cleansing.

2.19.4 Keep Lens Clean

The outer surface of the optics should be inspected and cleaned periodically using
an air stream from an air bulb. If dirt remains on the optical surface, use a soft cloth
or cotton swab dampened with a lens cleaning solution (available at the camera
stores).

All optical lenses should be swabbed using a circular motion. A small amount of
absorbent cotton wound on the end of a tapered stick makes a useful tool for
cleaning recessed optical surfaces.

Avoid using an excessive amount of solvents as this may cause problems with
optical coatings or cemented optics or the flowing solvent may pick up grease
making cleaning more difficult.

2.19.5 Cool and Dry Storage

Store the instrument in a cool, dry environment. Put the condenser back in the
storage box when not in use.

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3. General Specifications
3.1 Features
FEATURE SPECIFICATION
Optical System Infinity corrected
Nosepiece Reversed, ball bearing quintuple
Head Gemel type trinocular head, 30-degree inclined
Infinity Plan Objectives 4X, 10X, 20X, 40X (spring), 100X (spring, oil)
Focusing Low position coaxial focus system
Interpupillary Adjustment Range 2-3/16" - 3" (55-75mm)
Mechanical Tube Length 6-5/16" (160mm)
Mechanical Stage 8.5" x 5.9" (216mm x 150mm)
Stage Traveling Range 2.9" x 2" (75x50mm)
Focusing Range 0.95" (24mm)
Division of Fine Focusing 0.00003935" (0.001mm)
Illumination Built-in Kohler LED Illumination System
Condenser N.A. 1.25/0.22 Swing Achromatic Condenser
Power Supply 90V-240 Wide Voltage, CE Certified

3.2 Eyepiece Comparison

EYEPIECES T720 T720QC T720-TP T720-HD18 T720QC-TP T720QC-HD18

PL10X22mm ● ● ● ● ● ●
WF25x30mm ● ● ●

3.3 Eyepiece Parameters

Eyepieces
Magnification 5X 10X 15X 16X 20X 25X
Field of View Φ18 Φ18 Φ13 Φ15 Φ11 Φ9

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3.4 Objectives

Numerical
Aperture Magnification
Model (N.A.) Marks
Type Number Magnification Rating Medium (Color Ring)
PA4X Plan 4X 0.10 Air Red
PA10X Plan 10X 0.25 Air Yellow
Plan Objective
PA20X Plan 20X Air
(195mm)
PA40X Plan 40X 0.65 Air Light Blue
PA100X Plan 100X 1.25 Cedar Oil White

3.5 Darkfield Condensers

Dry Darkfield Oil Darkfield
SPECIFICATIONS Condenser Condenser
Model DK-DRY100 DK-OIL100
Numerical Aperture (N.A.) Rating 0.7 – 0.9 1.36 – 1.25
Mounting Size (Diameter) 37mm 37mm

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4. Parameters
4.1 Electrical System
90V-240 Wide Voltage, CE Certified

4.2 Microscope Parameters

Magnification 10x-2500x
Field of View Φ0.8mm~Φ4.5mm
Mechanical Tube Length 160mm
Object to Primary Image Distance 195mm
Fine Focusing Sensitivity 0.002mm

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5. Recommended Accessories
(Purchase separately. Please visit www.amscope.com and search with SKU #, for more
information.)

5.1 Eyepieces

5X Eyepieces 20X Eyepieces 25X Eyepieces

SKU: EP5X23 SKU: EP20X23 SKU: EP25X23

10X w/ Pointer 10X w/ Reticle

SKU: EP10X23P SKU: EP10X23R

5.2 Darkfield Condensers

Dry Darkfield Condenser Oil Darkfield Condenser

SKU: DK-DRY100 SKU: DK-OIL100

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5.3 Objective Lenses

Achromatic

2X 5X 20X 60X
SKU: A2X SKU: A5X SKU: A20X SKU: A60X

Plan Achromatic

Plan 4X Plan 10X Plan 40X Plan 100X

SKU: PA4X SKU: PA10X SKU: PA40X SKU: PA100X

5.4 Fuses

Fuses For
Microscopes
SKU: FS-M
T1AL 220V

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5.5 Cameras and Accessories

Cameras
To capture images, video, or view live display on a computer (PC/Mac OS X). Real-
Time Live Video Microscope Digital Camera, Reduction Lens, High Sensitivity
Sensor and Deluxe Calibration Kit.

350K 1.3 Mega 3 Mega 5 Mega 8 Mega 9 Mega 10 Mega

Pixel Pixel Pixel Pixel Pixel Pixel Pixel
SKU: SKU: SKU: SKU: SKU: SKU: SKU:
MU035 MU130 MU300 MU500 MU800 MU900 MU1000

Calibration Micrometer
Calibrate the camera software for on-screen measurements.
SKU: MR400

Charge-Coupled Device Television

CCD Digital CCD TV/Video 1920x1080 Full HD HDMI

(VGA, Trinocular Only) (Trinocular Only) (Trinocular Only)
SKU: CCD-MT SKU: CCD-NP SKU: HD1080
To view live display on a To view live display on a To view live display on a
computer monitor (VGA). television (RCA). computer monitor (VGA).

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5.6 Slides

Prepared

25 Slides 50 Slides 100 Slides 200 Slides

SKU: PS25W SKU: PS50 SKU: PS100A SKU: PS200

Blank

50 Blank Slides 100 Blank Slides 144 Blank Slides 300 Blank Slides
SKU: BS-100P- SKU: BS-144P- SKU: BS-300P-
SKU: BS-50P-C 100S-22 200S-22 300S

5.7 Cleaners

Sparkle Microscope 3 in 1 Professional 3 in 1 Cleaning Kit

Optical Lens Cleaner Cleaning Kit for for Microscopes,
SKU: CLS Microscopes, Cameras Cameras and
and Laptops LCD Screens
SKU: CK-I SKU: CK-II

5.8 Books

Complete Book of the The Ultimate Guide to The World of the

Microscope Your Microscope Microscope
(Science and
(What to look for) Ages 8-Adult Experiments)
Ages 8-Adult Ages 8-12
SKU: BK-CM SKU: BK-UG SKU: BK-WM

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6. Troubleshooting
6.1 Optical Issues
SYMPTOM
SYMPTOM CAUSE REMEDY
OPTICAL ISSUES
Turn the nosepiece until it clicks into
One side of the field of view is The nosepiece is misaligned. place.
darker Stains, dust, or dirt has
accumulated on the objectives Clean all lenses with lens cleaner or a lint
or eyepieces. free non-abrasive cloth.
Stains, dust, or dirt has Clean the slide or use a new specimen if
Obstructions are observed in accumulated on the specimen. sample is destroyed.
the field of view Stains, dust, or dirt has
accumulated on the objective,
eyepieces, or Barlow lens. Clean the lens.
Add a cover slip. The objectives are
designed for use with a 0.17mm cover
There is no cover slip on the slip, so it is a requirement to use one for
slide. proper images.
The cover slip is not standard Replace the cover slip with the
sized. appropriate 0.17mm thickness slip.
The immersion oil has Thoroughly clean the objective lens with
accumulated on the dry lens cleaner or a lint free non-abrasive
objective. cloth
No immersion oil is used with Use immersion oil for better clarity and
the 100X objective. resolution.
Used wrong oil. Use standard cedar wood oil.
Unclear Image Adjust the aperture to have the light just
The aperture is not open to an larger than
appropriate diameter. the size of the condenser.
Stains, dust, or dirt has Clean the lens with lens cleaner or a non-
accumulated on the inlet of the abrasive lint free cloth, as well as spray
head. with compressed air.
Adjust condenser height to the top of the
The condenser is not in the travel range, and then adjust down to
right position. focus image.
Initial focus was at too high a Start focusing with the lowest objective
magnification. and then switch to the higher ones.
The slide thickness at higher
magnifications will impact clarity if the
The slide is upside down slide is upside down.
Adjust the brightness knob to a higher or
The brightness adjustment lower setting for color
The color of the image is not knob is not in the right position. clarity.
accurate
Remove color filter if natural light is
No filter is used or filter is in desired, or insert desired
use. filter.

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SYMPTOM CAUSE REMEDY

OPTICAL ISSUES
One side of the field of Secure the slide to the stage with
view is dark or the image The specimen slide is not fixed. clips.
moves while focusing The nosepiece is not in the right Turn the nosepiece until it clicks into
position. place.
Adjust the iris diaphragm to allow the
light to be just larger than the
The iris diaphragm is too small. condenser.
The field of view is not Adjust condenser height to the top of
bright enough The condenser is not in the right the travel range, and then adjust
position. down to focus image.
Stains, dust or dirt has accumulated
on the condenser, objective, Clean the lens with lens cleaner or a
eyepieces, or base lens. non-abrasive lint free cloth.

6.2 Mechanical Issues

SYMPTOM CAUSE REMEDY
MECHANICAL ISSUES
The slide is not secured Adjust the slide to use the stage clips and secure
Unable to move the correctly. the sample.
slide smoothly. The mechanical stage is not Tighten the mechanical stage screws to better
properly secured. secure the stage.
The cover slip is not standard Replace the cover slip with the appropriate 0.17mm
sized. thickness slip.

The objective
touches the cover Be careful to avoid contact between objective and
slip. the slide when the limit stop is not engaged (unless
using the 100X objective with oil). To re-engage,
focus the sample, then lock the limit stop into place
The limit-stop is set too high to set maximum height at a safe but usable
or not engaged. distance.
Loosen it by adjusting the tension ring inside the
Focus-knob does coarse focus knob counterclockwise (close to the
not turn. arm of the microscope on the left of the
The tension knob is too tight. microscope).

Stage declines by Tighten it by adjusting the tension ring inside the

itself. coarse focus knob clockwise (close to the arm of
The tension knob is too loose. the microscope on the left of the microscope).
The coarse
focusing knob won’t Disengage the limit stop on the left side of the
raise the stage. Limit-stop is engaged. microscope inside the coarse focusing knob.
The fine focusing
knob won’t raise the Disengage the limit stop on the left side of the
stage. Limit-stop is engaged. microscope inside the coarse focusing knob.

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6.3 Electrical Issues

SYMPTOM CAUSE REMEDY
ELECTRICAL ISSUES

The bulb/light source Replace the bulb. This unit uses our BH-
flickers. The bulb is close to 6V20W with our 20w unit or our BH-
burning out. 6V30W for the 30W unit.
The microscope is Insert the plug into the wall socket to
unplugged. achieve electrical illumination.
Check the bulb by unscrewing the base
(remove eyepieces first to prevent falling
The microscope does not The bulb is not inserted out) door and ensuring that the bulb is
light up. correctly. inserted.
Replace the bulb. This unit uses our BH-
6V20W with our 20w unit or our BH-
The bulb burned out. 6V30W for the 30W unit.
Replace with fuse on the bottom of the
The fuse burned out. microscope.
Use the correct power supply (110v if
The fuse burns out 110v unit, 220v if 220v
frequently. unit), or get a voltage adapter to convert to
the proper electrical
The voltage is too high. system.
Use the correct power supply (110v if
110v unit, 220v if 220v unit), or get a
voltage adapter to convert to the proper
electrical
The voltage is too high. system.
The bulb burns out Use the correct wattage bulb for the unit.
frequently. Using a higher wattage than it is rated for
can damage your unit (melt components
with additional heat), so please be sure to
use the correct one. Damage from
incorrect usage is not covered under
Used wrong bulb. warranty.

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7. General Microscopy Guide

Microscopes come in a wide variety of types with many different features. Each
AmScope model is designed for specific uses and specific users. This glossary will help
illustrate what the variations mean and why they are useful.

Compound and Stereo Microscopes

Using a compound or a stereo microscope

depends on the specimen being studied.

Compound microscopes are best for smaller

transparent specimens, like slides and biological
subjects.
Compound microscopes show a two-dimensional
image of the specimen (usually reversed and
upside-down)

The common magnification range of our

compound microscopes is between 40X-1000X,
could be up to 2500X. You need a minimum of
400X to study cell structure.

Stereo microscopes are best for larger specimens

you cannot see through.

Stereo microscopes show a 3D image. Three

dimensional imaging is perfect for performing
dissections, repairing circuit boards, studying
fossils and gems or examining any specimen
where you want to use your hands.

The magnification range of our stereo microscopes

is between 2X to 225X.

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Base Lens

Base lenses direct the light source towards the specimen.

Binocular Head-Rotating

A rotating binocular head is designed for

maximum flexibility in viewing options.

Binocular microscope heads allow for the

easy adjustment of the interpupillary distance
(the distance between the eyes), by moving
the eyepieces toward or away from each
other.

The rotating feature allows for either forward

or reverse view, depending on whether you’d
rather look at the specimen or over it.

Condenser Lens

A condenser lens concentrates light on a specimen and

increases the resolution. An adjustable iris controls the diameter
of the beam of light entering the lens system. Abbe Condenser
lenses are specially designed to mount under the stage.

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Condenser Lens Adjustment Knob

The Condenser Lens Adjustment

Knob changes the distance
between the light condenser and
the base lens. This allows you to
control the concentration of the
light hitting your slide.

Darkfield Condenser Lens

A darkfield condenser lens produces bright images against a dark background for low
contrast biological specimens.
With brightfield lenses, a solid
beam of light illuminates the
objective lens. Darkfield condenser
lenses block the center of the
beam of light to produce a hollow
cone of light. Light does not enter
the objective lens directly. The
specimen scatters light, which then
enters the objective lens.

Brightfield Darkfield

Dimmer
Dimmers control the amount of light escaping from the base lens.

DIN/JIS
DIN is the Deutsches Institut für Normung, the German Institute for Standardization,
an international standards organization determining the "standard" for many types of
technology. JIS is the Japanese Industrial Standard, specifying the standards used
for industrial activities in Japan.

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Diopters

Small rings on the eyetubes, used to focus ocular

lenses (eyepieces).

Eye Guards
Eye Guards fit over the eyepieces, they are for
comfort and to protect the glass in the eyepiece.

Eyepieces
Eyepieces are also called ocular lenses. Eyepieces
come in many magnifications and you replace them
by swapping them out of the eyetubes.

The eyepieces magnify the specimen further from the

first magnification through the objective lens. Each
eyepiece is marked with 2 numbers. The first number
is the magnification and the second is the field of
view.

For example, an eyepiece marked WF10/20 has a

magnification of 10X and a field of view of 20mm.

Eyetubes

Eyetubes are also called ocular tubes. Eyetubes

house the eyepieces and camera adapters. You
change eyepeices by removing them from the
eyetubes and replacing them with the new ones.

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Field of View and Working Distance

Field of view is how much of the specimen you can see

through the eyepiece. The linear field of view of the
eyepiece is divided by the magnification of the objective.
The higher the magnification, the smaller the field of
view.

Working distance is the distance between the

bottom of objective lens and the stage. You
change the working distance when you use the
coarse focusing knob.

Together, working distance and field of view

determine how much of the specimen you see
and how closely.

Focusing Knobs

Coarse Focus: This is the large knob on the side of the

microscope that moves the objective lens up and down. It
is used in conjunction with the fine focus. Do not use this
knob with the 100X objective lens.

Fine Focus: The outer knob, used to fine-tune the focus of

a specimen in conjunction with the coarse focus.

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Head Types

Monocular

Monocular microscope heads have one eyepiece.

Binocular

Binocular microscope heads have two eyepieces.

Trinocular

Trinocular microscope heads have two eyepieces and a photo port

for a camera. This allows you to look at a specimen with a camera
without removing an eyepiece.

Multi-head

Multi head microscopes are specially designed for three-person

observation. Ideal for teaching and/or training purposes. It has a
binocular head and two monocular heads.

Simul-Focal

Simul-Focal heads mitigate the amount of re-focusing needed when

you change your view from the eyepieces to the monitor.

Perfect for lectures, teaching demonstrations, clinical examinations

and laboratory applications.

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Immersion Oil
A special oil used with the 100X objective to concentrate the light and increase
the resolution of the image. At high magnifications the field of view is so small
that very little light passes. The light refracts, scatters and produces a dark
image.

A drop of oil is placed on the slide’s cover slip and the objective is lowered until
it touches the oil. The oil will match the specimen’s light refracting index to the
lens, adding more light to the specimen, which improves resolution and clarity.

Interpupillary Distance
Interpupillary Distance is the distance between your eyes. AmScope uses 2 types of
systems to change interpupillary distance.

Siedentopf heads adjust the interpupillary distance by rotating the

eyetubes around a central axis. A Siedentopf head changes the
interpupillary distance without changing the tube distance.

Jentsch heads adjust the interpupillary distance by moving the

eyetubes closer together or farther apart in a linear fashion. The
tube length changes when changing interpupillary distance, so the
user compensates by adjusting the focus of the eyepieces to
correspond to the new interpupillary distance.

Iris or Diaphragm

The diaphragm or iris is located under the stage and is an apparatus

that can be adjusted to vary the intensity, and size, of the cone of
light that is projected through the slide.

The iris adjustment slider allows you to adjust the opening of the iris.

Lenses
Microscopes have a two lens system, objective and ocular.

The Objective Lens is the lens closest to the specimen or object.

The Ocular Lens (Eyepiece) is the lens closest to the eye.

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Light Bulbs-Caution
1. Turn off the microscope when not in use to preserve the life of the bulb.
2. Never touch a light bulb directly, especially when it’s turned on.
a. The light bulbs can get hot enough to burn skin. Wait until the bulb has cooled
completely before handling it.
b. The oil from your skin will create a hot spot on the bulb which will damage the
glass, dramatically shortening the life of the bulb.
c. When changing light bulbs cover skin with cloth or paper to handle the bulb.
This will protect both you and the bulb.
3. Hot bulbs will not damage the microscope. AmScope’s microscopes are designed
specifically to handle the heat output of the model’s bulb.

Lighting-Types
Tungsten
Tungsten light bulbs are the most economical, providing a reliable
source of light. They burn hotter than the other bulbs, but are the best
option for certain microscopes.

Fluorescent
Fluorescent bulbs burn cooler and brighter than tungsten bulbs. The
bulbs are more expensive than tungsten, but
last longer.

LED
LED light bulbs burn the coolest of all the bulbs and last the longest.
The bulbs are also the most expensive. Depending on use, LED
lights should last about 40,000 hours.

Halogen
Halogen light bulbs produce the most amount of light. The light is
very white and concentrated; the bulbs can get very hot and must be
cooled regularly. Depending on use, halogen light bulbs can last
anywhere from 1 week to 6 months.

Fiber Optic
Fiber optical lighting uses a system of flexible transparent fibers
made of plastic or glass. The light is transmitted between the two
ends of the fiber, allowing for greater illumination in confined spaces.

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Limit Stop Knob

The limit stop is designed to prevent impact between objective and slide. You may
loosen the limit-stop knob in order to give yourself the full range of motion for fine tuning
the focus when using the 40X and 100X objective lenses.

When the limit stop is off you will be able to damage the microscope or the glass slide.
For safety, engage the limit stop once you have it close or in contact with the objective
(if using oil contact is required).

Magnification
Microscopes have a two lens system. Total Magnification is the power of two lenses
multiplied together.

E.g.: (10X Eyepieces) x (4X Objective) = 40X Total Magnification

Mechanical Stage

A Mechanical Stage is a flat mechanism that sits on top of

the stage and allows the viewer to move a specimen small
distances - a task that is otherwise difficult at higher
magnifications. Most mechanical stages are equipped with
an X and Y axis so the viewer can see how far the slide
has moved.

Mechanical Stage Control

The mechanical stage control moves the stage,

mechanically moving the slide along an X and Y axis for
optimal positioning.

The top ring moves the stage forward and backward. The
bottom ring moves the stage from left to right.

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Nosepiece

This circular structure is where the different objective lenses are

screwed in. To change the magnification power, rotate the turret.

Numerical Aperture (N.A.)

N.A. is a rating for the resolution of the objective lens. The N.A. rating ranks the
objective lens’ ability to capture light and show fine detail.

Lens with larger numerical apertures capture finer details than lenses with smaller
numerical apertures. Generally, lenses with larger numerical apertures create a brighter
image, but the depth of field will be shallower.

N.A. Rating
Average
Objective Numerical
Lens Aperture
4X 0.1
10X 0.25
40X 0.65
100X 1.25

Parcentered
When changing objectives, the image of the specimen stays centered. Most compound
microscopes are parcentered.

Parfocal
When changing objectives, the image of the specimen stays in focus without
needing to adjust the coarse focusing knob. Not all compound microscopes are
parfocal.

Tension Knob

The large, innermost ring on the focusing knob.

Adjusts the tension of the focusing knobs.

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Trinocular Port

A trinocular microscope has three viewing ports.

There are two eyepieces and a third port for
photography and video.

The trinocular C-Mount adapter.

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