National Veterinary Services Laboratories

NAHLN Document List

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Disease Number and Document Document Title
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FM-FADDL- ASF PCR Control QC Summary
ASF NVSL-FM-1094.05
0052 Sheet
African Swine Fever rPCR Assay on
FM-FADDL-
ASF NVSL-FM-1129.06 the Applied Biosystems 7500 Real-
0087
time PCR System Worksheet
African Swine Fever rPCR Assay on
FM-FADDL- the Applied Biosystems™
ASF NVSL-FM-1134.03
0092 QuantStudio™ 5 Real-Time PCR
System Worksheet
Extraction of Total DNA from
SOP-FADDL- Tissues, Tissue Culture Supernatant,
ASF NVSL-SOP-0591.04
0067 and Whole Blood Using a Qiagen
DNeasy® Blood and Tissue Mini Kit
Preparation, Performance and
Interpretation of the African Swine
SOP-FADDL-
ASF NVSL-SOP-0646.05 Fever rPCR Assay on the Applied
0072
Biosystems 7500 Real-time PCR
System
Preparation, Performance, and
Interpretation of the African Swine
ASF NVSL-SOP-0650.05 SOP-PS-0002 Fever rPCR Assay on the Applied
Biosystems® QuantStudio 5 Real-
time PCR System
African Swine Fever and Classical
Swine Fever Real-Time RT-PCR
Assay on the Applied Biosystems ™
ASF, CSF NVSL-FM-2124.01 N/A
QuantStudio 5 ™ and the Applied
Biosystems ™ 7500 Real-Time PCR
Systems
African Swine Fever (ASF)/Classical
MAN-NAHLN-
ASF, CSF NVSL-MAN-0100.02 Swine Fever (CSF) Surveillance -
0006
NAHLN Laboratory Manual
Preparation, Performance, and
Interpretation of the African and
Classical Swine Fever Real-Time
ASF, CSF NVSL-SOP-1201.02 N/A Reverse-Transcription Multiplex
Polymerase Chain Reaction (rRT-
PCR) on the Applied Biosystems™
QuantStudio™ 5 Real-Time System
Preparation, Performance, and
Interpretation of the African and
Classical Swine Fever Real-Time
ASF, CSF NVSL-SOP-1203.02 WI-AV-0035 Reverse-Transcription Multiplex
Polymerase Chain Reaction (rRT-
PCR) on the Applied Biosystems™
7500 Real-Time System
ASF, CSF, FM-FADDL- Recovery of Sample from a Swab
NVSL-FM-1080.05
FMD 0038 Worksheet

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ASF, CSF, FM-FADDL- Recovery of Sample from Tissue
NVSL-FM-1081.05
FMD 0039 Worksheet
Nucleic Acid Extraction Using the
ASF, CSF, FM-FADDL- MagMAX™ CORE Nucleic Acid
NVSL-FM-1127.04
FMD 0085 Purification Kit on a Magnetic Particle
Processor Worksheet
Nucleic Acid Extraction Using the
ASF, CSF, FM-FADDL- MagMAX™ Pathogen RNA/ DNA Kit
NVSL-FM-1128.06
FMD 0086 on a Magnetic Particle Processor
Worksheet
Preparation of Tissues, Tissue
ASF, CSF, SOP-FADDL-
NVSL-SOP-0589.03 Homogenates, and Swabs for
FMD 0016
Nucleic Acid Extraction
Nucleic Acid Extraction Using the
ASF, CSF, SOP-FADDL- MagMAX™ CORE Nucleic Acid
NVSL-SOP-0643.04
FMD 0069 Purification Kit on a Magnetic Particle
Processor
Nucleic Acid Extraction using the
ASF, CSF, SOP-FADDL-
NVSL-SOP-0644.04 MagMAX Pathogen RNA/DNA kit on
FMD 0070
a Magnetic Particle Processor
Nucleic Acid Extraction Using the
ASF, CSF, SOP-FADDL- INDICAL BIOSCIENCE IndiMag
NVSL-SOP-0645.02
FMD 0071 Pathogen Kit on a Magnetic Particle
Processor
Production and Use of a Reference
ASF, CSF,
NVSL-SOP-1059.05 N/A Panel for Quality Control of Primers
FMD
and Probes for PCR
Work Instructions for Use of a
ASF, CSF, Reference Panel for QA/QC Testing
NVSL-WI-1174.03 WI-RVS-0003
FMD of Primers and Probes for Real-time
PCR Applications
Extraction of Total DNA from
Tissues, Tissue Culture Supernatant,
FM-FADDL-
ASF, FMD NVSL-FM-1083.04 and Whole Blood Using a Qiagen
0041
DNeasy® Blood and Tissue Mini Kit
Worksheet
Redirection of Bovine Spongiform
BSE NVSL-SOP-0886.02 SOP-PS-0048
Encephalopathy Samples
Quality Assurance: Proficiency
Testing and Quality Monitoring of
BSE NVSL-SOP-0924.02 SOP-PS-0051
BSE ELISA Contract Laboratories by
NVSL
Protocol for Bovine Spongiform
Encephalopathy (BSE) Approved
National Animal Health Laboratory
BSE NVSL-SOP-0927.02 SOP-RVS-0028 Network (NAHLN) Laboratories to
Receive and Test Bovine Brain
Samples and Report Results for BSE
Surveillance
FM-FADDL- CSF PCR Control QC Summary
CSF NVSL-FM-1093.05
0051 Sheet

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Disease Number and Document Document Title
Revision Number
Classical Swine Fever and Foot and
Mouth Disease Real-Time RT-PCR
Assay Using the TaqMan® Fast
CSF, FMD NVSL-FM-1126.04 NVSL-FM-1126
Virus 1-Step Master Mix on the
Applied Biosystems Quant Studio 5
Real-time PCR Platform Worksheet
Classical Swine Fever and Foot and
Mouth Disease rRT-PCR Using the
FM-FADDL-
CSF, FMD NVSL-FM-1130.07 TaqMan® Fast Virus 1-Step Master
0088
Mix on the Applied Biosystems 7500
Real-time PCR Platform Worksheet
Nucleic Acid Extraction Using the
FM-FADDL- INDICAL BIOSCIENCE IndiMag
CSF, FMD NVSL-FM-1131.03
0089 Pathogen Kit on a Magnetic Particle
Processor Worksheet
Classical Swine Fever and Foot and
Mouth Disease rRT-PCR Using the
SOP-FADDL-
CSF, FMD NVSL-SOP-0642.07 TaqMan® Fast Virus 1-Step Master
0068
Mix on the Applied Biosystems 7500
Real-time PCR Platform
Classical Swine Fever and Foot and
Mouth Disease rRT-PCR Using the
SOP-FADDL- TaqMan® Fast Virus 1-Step Master
CSF, FMD NVSL-SOP-0647.03
0073 Mix on the Applied Biosystems
QuantStudio 5 Real-time PCR
Platform
Chronic Wasting Disease (CWD)
CWD NVSL-SOP-0883.02 SOP-PS-0010 Enzyme-Linked Immunosorbent
Assay (ELISA)
CWD
Information on "Initial Reactives” for
ELISA, BSE NVSL-FM-1583.05 NVSL-FM-1583
TSE ELISA
ELISA
Detection of Scrapie and Chronic
CWD IHC /
NVSL-SOP-0878.03 SOP-PS-0007 Wasting Disease by
Scrapie IHC
Immunohistochemistry (IHC)
Fin Fish – VI
24 Well Plate Virus Isolation
(VHSV & NVSL-FM-0393.03 FM-BPA-0005
Worksheet
SVCV)
Fin Fish – VI
(VHSV & NVSL-FM-0408.04 FM-BPA-0020 Virus Isolation Worksheet
SVCV)
Fin Fish – VI
(VHSV &
Isolation of Viral Agents from Finfish
SVCV), NVSL-SOP-0285.05 SOP-BPA-0018
in Tissue Cell Culture
VHS-PCR,
ISA-PCR

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Disease Number and Document Document Title
Revision Number
Fin Fish – VI
(VHSV &
Disruption of Fish Tissue by Micro-
SVCV), NVSL-SOP-0324.03 SOP-BPA-0054
Bead Beating
VHS-PCR,
ISA-PCR
FM-FADDL- FMD PCR Control QC Summary
FMD NVSL-FM-1092.04
0050 Sheet
Foot and Mouth Disease Virus and
Senecavirus A Multiplex rRT-PCR
FMD/SVA FM-FADDL- Using the TaqMan® Fast Virus 1-
NVSL-FM-1132.03
Multiplex 0090 Step Master Mix on the Applied
Biosystems 7500 Real-time PCR
Platform Worksheet
Preparation, Performance and
Interpretation of the Foot and Mouth
FMD/SVA SOP-FADDL- Disease Virus and Senecavirus A
NVSL-SOP-0648.02
Multiplex 0075 Multiplex rRT-PCR Assay on the
Applied Biosystems 7500 FAST Real-
time PCR System
Isolation of Avian Influenza and Avian
IAV-A NVSL-SOP-0018.05 SOP-AV-0068 Paramyxoviruses Viruses in Chicken
Embryos from Avian Species
Quick Reference for Real-Time RT-
PCR Influenza A Detection using the
IAV-A NVSL-WI-0042.05 WI-AV-0050
IAV M 2009 Assay (for AB7500 Fast
and Cepheid SmartCycler II)
NVSL Testing Guidance for Post
IAV-A NVSL-WI-0048.06 WI-AV-0051
C&D Environmental Samples
Quick Reference for Real-Time RT-
PCR Influenza A Assays for Subtype
IAV-A NVSL-WI-0053.02 WI-AV-0052
H5 or H7 Detection (for AB7500 Fast
and Cepheid Smart Cycler)
IAV-A, IAV- NVSL-Process- Process-DVL- Influenza A Test Comparison for
S 0002.02 0001 Avian and Swine
Evaluation and Quality Control
IAV-A, IAV-
NVSL-WI-0054.04 WI-BPA-0032 Testing of Real Time PCR Primers
S, ND
and Probes
IAV-A, IAV- RNA Extraction Using a Magnetic
NVSL-WI-0055.02 WI-BPA-0048
S, ND Particle Processor
RRT-PCR Primer and Probe Quality
IAV-A, ND NVSL-FM-0039.04 FM-AV-0038
Control Worksheet
NVSL-Process- Process-AV- Testing and Confirmation for Avian
IAV-A, ND
0001.02 0001 influenza and Newcastle Disease
Real-time RT-PCR Detection of
IAV-A, ND NVSL-SOP-0068.05 SOP-BPA-0010 Influenza A and Avian Paramyxovirus
Type-1

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Disease Number and Document Document Title
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Programming and Analysis for the
IAV-A, ND NVSL-WI-0038.06 WI-AV-0037 Cepheid SmartCycler Real-Time
Instrument
Programming and Analysis for the
IAV-A, ND NVSL-WI-0039.05 WI-AV-0038 Roche LightCycler® 480 Real-Time
Instrument
Programming and Analysis for the
IAV-A, ND NVSL-WI-0040.04 WI-AV-0039 Applied Biosystems 7900 Real-Time
Instrument
Real-Time RT-PCR for the Detection
IAV-S NVSL-SOP-0289.03 SOP-BPA-0019 of Influenza A Virus in Swine (IAV-S)
in Clinical Samples
Amplification and Purification of PCR
Products for Direct Sequencing of
IAV-S NVSL-SOP-0290.03 SOP-BPA-0023 Hemagglutinin, Neuraminidase, and
Matrix Genes of Swine Influenza
Virus
IAV-S NVSL-SOP-0294.02 SOP-BPA-0044 Swine Influenza Virus Isolation
Real-Time RT-PCR for Subtyping
IAV-S NVSL-SOP-0315.04 SOP-BPA-0052 Type A Swine Influenza Virus in
Clinical Samples
Minimum Requirements for
WI-NAHLN-
IAV-S NVSL-WI-0209.03 Submitting NAHLN Swine Influenza
0011
Virus Sequencing to GenBank
ISA-PCR NVSL-FM-0479.05 FM-BPA-0091 ISAV RT-PCR
ISAV VetMAX plus - Multiplex One
ISA-PCR NVSL-FM-0481.03 FM-BPA-0093
Step RT-PCR
Dissection of Fish for Detection of
ISA-PCR NVSL-SOP-0323.02 SOP-BPA-0053 Viruses During Large-Scale,
Targeted Surveillance for ISAV
Infectious Salmon Anemia Real-time
ISA-PCR NVSL-SOP-0326.03 SOP-BPA-0056
RT-PCR - segment 8 Snow primers
Infectious Salmon Anemia RT-PCR -
ISA-PCR NVSL-SOP-0327.03 SOP-BPA-0060
Segment 6 and 8
ISA-PCR, KingFisher AMB1836 Extraction
NVSL-FM-0480.03 FM-BPA-0092
VHS-PCR Worksheet for Aquaculture
FM-NAHLN- NAHLN Quality Standard
N/A NVSL-FM-1347.04
0051 Harmonization Checklist
MAN-NAHLN- NAHLN Laboratory Guide for Foreign
N/A NVSL-MAN-0028.04
0002 Animal Disease (FAD) Investigations
Policy-NAHLN-
N/A NVSL-Policy-0036.02 NAHLN Laboratory Performance
0006
NAHLN Quality Standard -
Policy-NAHLN-
N/A NVSL-Policy-0037.03 Harmonization of AAVLD and
0007
ISO/IEC 17025:2017
WI-NAHLN- NAHLN Laboratory Controlled Copy
N/A NVSL-WI-0702.05
0038 SOP Responsibilities

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Disease Number and Document Document Title
Revision Number
Registering for a Proficiency Test
N/A NVSL-WI-0721.04 WI-RVS-0003 (PT) Event in the APHIS Laboratory
Portal
WI-NAHLN-
N/A NVSL-WI-0729.10 NAHLN Test Reference Chart
0038
N/A NVSL-WI-1781.01 N/A NAHLN Electronic Messaging Guide
Quick Reference for Real-Time RT-
PCR Avian Paramyxovirus Type-1
ND NVSL-WI-0041.04 WI-AV-0045
Detection (for AB7500 Fast and
Cepheid SmartCycler)
VHS-PCR NVSL-FM-0482.04 FM-BPA-0094 VHSV rRT-PCR Worksheet
Viral Hemorrhagic Septicemia Virus
VHS-PCR NVSL-SOP-0331.02 SOP-BPA-0063
Real-time RT-PCR
VHS-PCR, MagMAX AM1836 Extraction
NVSL-FM-0458.04 FM-BPA-0070
ISA-PCR Worksheet for Aquaculture
VHS-PCR, Extraction of Nucleic Acid (DNA and
NVSL-SOP-0325.04 SOP-BPA-0055
ISA-PCR RNA) from Fish Tissues - AM1836
Vesicular Stomatitis Complement
VSV NVSL-FM-0405.05 NVSL-FM-0405
Fixation Bench Record
Complement Fixation Assay for
VSV NVSL-FM-0406.04 FM-BPA-0018 Vesicular Stomatitis Complement
Titration Worksheet
Complement Fixation Assay for
VSV NVSL-FM-0407.04 FM-BPA-0019 Vesicular Stomatitis
Hemolysin Titration Worksheet
Washing & Standardization of Sheep
VSV NVSL-FM-0420.04 FM-BPA-0032
Red Blood Cells
Vesicular Stomatitis PCR Worksheet
VSV NVSL-FM-0483.04 FM-BPA-0095
Using Fast Virus 1-Step Kit
MagMAX Express 96 Extraction
VSV NVSL-FM-0485.04 FM-BPA-0097
Worksheet (200µl sample)
Complement Fixation Test for
VSV NVSL-SOP-0281.03 SOP-BPA-0011 Detection of Antibodies to Vesicular
Stomatitis Virus
Reagent Preparation and Evaluation
VSV NVSL-SOP-0282.03 SOP-BPA-0014 for the Vesicular Stomatitis
Complement Fixation Assay
Real-time Reverse-Transcriptase
Polymerase Chain Reaction for the
SOP-FADDL-
VSV NVSL-SOP-0334.04 Detection of Vesiculovirus Nucleic
0014
Acids Using the ABI 7500 Detection
System
WI-NAHLN- RNA Extraction of Vesicular
VSV NVSL-WI-0225.02
0030 Stomatitis Using MagMAX Bead Kits

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Document
Document Number Document Title
Revision
Intravenous Pathogenicity Assessments for Avian Influenza Virus in
NVSL-SOP-0014 .07
Chickens
Isolation of Avian Influenza and Avian Paramyxoviruses Viruses in
NVSL-SOP-0018 .05
Chicken Empryos from Avian Species
Agar Gel Immunodiffusion Test to Detect Antibodies to Avian
NVSL-SOP-0066 .01
Encephalomyelitis Virus
Real-time RT-PCR Detection of Influenza A and Avian
NVSL-SOP-0068 .05
Paramyxovirus Type-1
Avian Influenza Real-Time RT-PCR Testing for Qualified NPIP
NVSL-SOP-0074 .02
Primary Breeder Authorized Laboratories
NVSL-SOP-0076 .05 Quality Control Testing of Taylorella spp. Media
NVSL-SOP-0082 .05 Pulsed Field Gel Electrophoresis (PFGE) on Taylorella isolates
NVSL-SOP-0089 .05 Salmonella pullorum Microagglutination Test
NVSL-SOP-0091 .04 Salmonella pullorum Tube Agglutination Test
NVSL-SOP-0180 .07 Taylorella spp. Isolation and Identification
NVSL-SOP-0181 .05 Taylorella spp. Direct Fluorescent Antibody Test
Real-time Polymerase Chain Reaction Test for Rapid Identification
NVSL-SOP-0183 .05
and Speciation of Taylorella spp.
Complement Fixation Test for Detection of Antibodies to Vesicular
NVSL-SOP-0281 .03
Stomatitis Virus
NVSL-SOP-0292 .01 Virus Isolation Procedure for Pseudorabies Virus (PRV)
Virus Neutralization Test for Detection of Antibodies Against
NVSL-SOP-0306 .06
Pseudorabies Virus
Serology Testing for Pseudorabies in the Diagnostic Virology
NVSL-SOP-0310 .05
Laboratory
Dissection of Fish for Detection of Viruses During Large-Scale,
NVSL-SOP-0323 .02
Targeted Surveillance for ISAV
NVSL-SOP-0324 .03 Disruption of Fish Tissue by Micro-Bead Beating
Extraction of Nucleic Acid (DNA and RNA) from Fish Tissues -
NVSL-SOP-0325 .04
AM1836
Infectious Salmon Anemia Real-time RT-PCR - segment 8 Snow
NVSL-SOP-0326 .03
primers
NVSL-SOP-0327 .03 Infectious Salmon Anemia RT-PCR - Segment 6 and 8
NVSL-SOP-0331 .02 Viral Hemorrhagic Septicemia Virus Real-time RT-PCR
Serotype Identification of Orbiviruses (BTV, EHDV, and AHSV) by
NVSL-SOP-0533 .03
RT-PCR
NVSL-SOP-0536 .03 Isolation of Bluetongue Virus
NVSL-SOP-0538 .02 Equine Arteritis Virus Isolation
NVSL-SOP-0541 .02 General Protocol for Isolation of Equine Viruses
NVSL-SOP-0558 .02 Microtiter Virus Neutralization Test for Equine Viral Arteritis
Requirements for Equine Infectious Anemia Agar Gel
NVSL-SOP-0562 .03 Immunodiffusion (Coggins) and Enzyme-Linked Immunosorbent
Assay Testing at APHIS-Approved Laboratories
Virus Neutralization Test for Bluetongue and Epizootic Hemorrhagic
NVSL-SOP-0564 .02
Disease Viruses
Real-Time RT-PCR Assays for Detection of Bluetongue, Epizootic
NVSL-SOP-0569 .06
Hemorrhagic Disease and African Horse Sickness Viruses
Procedure for Performing Miscellaneous Gel-based PCR Assays:
NVSL-SOP-0571 .03
EAV; Bunyavirus; and EHV-2, -3, & -5

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Extraction of Total DNA from Tissues, Tissue Culture Supernatant,
NVSL-SOP-0591 .04 and Whole Blood Using a Qiagen DNeasy® Blood and Tissue Mini
Kit
Nucleic Acid Sequencing of the Foot and Mouth Disease S
NVSL-SOP-0616 .01
Fragment
NVSL-SOP-0617 .02 Nucleic Acid Sequencing of the Foot and Mouth Disease L Fragment
NVSL-SOP-0618 .01 Nucleic Acid Sequencing of the Foot and Mouth Disease P1 Region
NVSL-SOP-0619 .01 Nucleic Acid Sequencing of the Foot and Mouth Disease 3’ Region
Classical Swine Fever and Foot and Mouth Disease rRT-PCR Using
NVSL-SOP-0642 .07 the TaqMan® Fast Virus 1-Step Master Mix on the Applied
Biosystems 7500 Real-time PCR Platform
Nucleic Acid Extraction using the MagMAX Pathogen RNA/DNA Kit
NVSL-SOP-0644 .04
on a Magnetic Particle Processor
NVSL-SOP-0659 .03 Testing Requirements for Culture Media; Brucella spp. Isolation
NVSL-SOP-0660 .03 Isolation: Brucella spp.
NVSL-SOP-0662 .03 Evaluation of Individual Lots of Brucella Ring Test Antigen
NVSL-SOP-0664 .03 Procedure for the Propagation of Brucellaphages
NVSL-SOP-0668 .03 AMOS PCR
NVSL-SOP-0670 .04 Identification of Brucella species
16s rDNA, rpoB, hsp65, ITS Sequencing for Bacterial Species and
NVSL-SOP-0682 .04
Prp Sequencing for Scrapie susceptibility genotyping.
NVSL-SOP-0690 .06 VersaTrekTM Liquid Culture of Mycobacterium species
HPC Decontamination of Tissues Submitted for Mycobacteria
NVSL-SOP-0692 .06
Culture
HPC Decontamination of Fecal Swabs and Feces Submitted for
NVSL-SOP-0693 .04
Mycobacteria Culture
NVSL-SOP-0695 .02 Procedures for Brucella Milk Ring Tests
NVSL-SOP-0696 .07 NaOH Decontamination Method for Mycobacterial Culture
Direct PCR Assay from Feces for Detection of Mycobacterium avium
NVSL-SOP-0697 .02 subsp. paratuberculosis Using Magmax Core Mechanical Lysis and
Purification Kit
NVSL-SOP-0701 .07 Inactivation of Brucella
NVSL-SOP-0708 .02 Isolation of Mycobacteria Using Solid Media
NVSL-SOP-0710 .05 MGIT™ 960 Liquid Culture of Mycobacteria species
Detection of Scrapie and Chronic Wasting Disease by
NVSL-SOP-0878 .03
Immunohistochemistry (IHC)
NVSL-SOP-0882 .03 Scrapie Enzyme-Linked Immunosorbent Assay (ELISA)
Protocol for Bovine Spongiform Encephalopathy (BSE) Approved
National Animal Health Laboratory Network (NAHLN) Laboratories to
NVSL-SOP-0927 .02
Receive and Test Bovine Brain Samples and Report Results for
BSE Surveillance
Detection of Mycobacterial Nucleic Acid in Formalin-Fixed Paraffin-
NVSL-SOP-0930 .04
Embedded Tissues by Polymerase Chain Reaction
Preparation of Antiserum for the Avian Influenza Agar Gel
NVSL-SOP-0963 .01
Immunodiffusion Test
Preparation of Optimal Dilutions for Antigen and Antiserum for the
NVSL-SOP-0965 .02
Avian Influenza Agar Gel Immunodiffusion Test
Standard Operating Procedure (SOP) for Cell Culture Media
NVSL-SOP-1033 .08
Preparation
Standard Operating Procedure (SOP) for Production of Virus Stocks
NVSL-SOP-1035 .07
Used in the Various Virus Neutralization (VN) Tests

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Standard Operating Procedure (SOP) for Quality Control Testing of
NVSL-SOP-1047 .02 Virus Stocks Prepared by the Reagents and Vaccine Services
Section
Classical Swine Fever 96 Well Plate Preparation for the
NVSL-SOP-1050 .05
Immunoperoxidase Test
Enzyme Linked Immunosorbent Assay (ELISA) for Titrating Rabbit
NVSL-SOP-1051 .06
and Guinea Pig Antisera Against Vesicular Disease Agents
Enzyme Linked Immunosorbent Assay (ELISA) for Testing
NVSL-SOP-1052 .05
Specificity of Rabbit Antisera Against Vesicular Disease Agents
Enzyme Linked Immunosorbent Assay (ELISA) for Testing
NVSL-SOP-1053 .05
Specificity of Guinea Pig Antisera Against Vesicular Disease Agents
Standard operating Procedure for Foot-and Mouth Disease (FMD)
NVSL-SOP-1062 .07
RT-qPCR Proficiency Panel Assembly and Distribution
Standard operating Procedure for Classical Swine Fever (CSF) rRT-
NVSL-SOP-1069 .06
PCR Proficiency Panel Assembly and Distribution
Standard Operating Procedure (SOP) of Demonstration of
NVSL-SOP-1071 .06 Contagious Bovine Pleuropneumonia in Foreign Animal Disease
Diagnostician Course
Standard Operating Procedure for the Application and Authorization
NVSL-SOP-1077 .03 for the Use of Biological Materials Containing Rinderpest Virus
(RPV)
Handling, packaging, transfer and shipping of Rinderpest Virus
NVSL-SOP-1078 .02 (RPV) containing Materials from the Reagents and Vaccine Services
Section (RVSS) RPV holding Facility.
General Procedures for Manipulating Rinderpest virus (RPV)-
NVSL-SOP-1080 .02 Containing Materials in the Reagents and Vaccines Service Section
(RVSS) of the Foreign Animal Disease Laboratory (FADDL)
Workflow for Receipt and Transfer of DNA Materials for NaOH
NVSL-SOP-1084 .03
Safety Treatment
Disinfectants and Cleaners Used in the Reagents and Vaccines
NVSL-SOP-1085 .06
Services Section (RVSS)
Standard Operating Procedure (SOP) for Preparation of Vesicular
NVSL-SOP-1087 .05
Exanthema of Swine Virus (VESV) Slides
Production of Antisera against Antigens of Foot and Mouth Disease
NVSL-SOP-1092 .05 Virus (FMDV), Swine Vesicular Disease Virus (SVDV), and Vesicular
Stomatitis Virus (VSV)
Workflow for Receipt, Process and Transfer of DNA Materials for
NVSL-SOP-1094 .05
NaOH Safety Treatment using Safety Treatment Database
Solid Phase Competition ELISA for Detecting Antibodies against
NVSL-SOP-1095 .03
Foot-and-Mouth Disease Virus
Production of Recombinant Foot-and-Mouth Disease Virus (FMDV)
NVSL-SOP-1101 .04 3D RNA Dependent RNA Polymerase for Virus Infection Associated
Antigen (VIAA) Test
Propagation of Foot and Mouth Disease Virus (FMDV), Swine
NVSL-SOP-1102 .03 Vesicular Disease Virus (SVDV) and Vesicular Stomatitis Virus
(VSV) in Roller Bottles for Antigen Purification
Standard Operating Procedure (SOP) for Preparation of African
NVSL-SOP-1106 .03
Swine Fever Virus (ASFV) Slides
Production of Binary Ethyleneimine (BEI) Inactivated Positive
NVSL-SOP-1107 .03
Amplification Controls (PAC) and Positive Extraction Controls (PEC)
NVSL-SOP-1108 .03 Purification of Vesicular Stomatitis Virus (VSV) Antigen
Inactivation of African Swine Fever Virus (ASFV) in Tissue Culture
NVSL-SOP-1109 .05
Supernatant using 3mM Binary Ethylenimine (BEI)

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Preparation, Performance, and Interpretation of a Real-Time PCR
NVSL-SOP-1111 .03 Assay for the Detection of Capripoxviruses on ABI 7500 Fast
Thermocycler Using TaqMan Fast Virus One-step Master Mix
Complement Fixation Test for Detection of Antibodies to Babesia
NVSL-SOP-1116 .03
caballi and Theileria (Babesia) equi—Microtitration Test
Indirect Fluorescent Antibody Test for Detection of Antibodies to
NVSL-SOP-1117 .07
Babesia caballi and Theileria (Babesia) equi
Complement Fixation Test for Detection of Antibodies to Anaplasma
NVSL-SOP-1123 .02
marginale—Microtitration Test
NVSL-SOP-1125 .03 Johne's Complement Fixation Test
Complement Fixation Test for Detection of Antibodies to Brucella
NVSL-SOP-1128 .03
abortus and Brucella suis
Plate Agglutination Test for Detection of Antibodies to Brucella
NVSL-SOP-1129 .07
abortus/suis
Buffered Acidified Plate Antigen Test for Detection of Antibodies to
NVSL-SOP-1130 .05
Brucella
Tube Agglutination Test for Detection of Antibodies to Brucella
NVSL-SOP-1132 .04
abortus/suis
Performance of ID Screen Brucellosis Serum Indirect Multi-species
NVSL-SOP-1136 .05
ELISA
NVSL-SOP-1140 .05 Card Test for Detection of Antibodies to Brucella
NVSL-SOP-1142 .05 Anaplasma Antibody Test Kit, cELISA v2
NVSL-SOP-1144 .11 Brucella Fluorescence Polarization Assay (FPA) Using Tubes
NVSL-SOP-1145 .04 VMRD cELISA Kits for Babesia caballi and Babesia equi
NVSL-SOP-1151 .06 IDEXX Johne's Disease ELISA Kit
NVSL-SOP-1152 .05 Contagious Equine Metritis Complement Fixation Test
NVSL-SOP-1154 .04 IDEXX ELISA Antibody Test Kits for Mycobacterium bovis
NVSL-SOP-1158 .04 Johne's Agar Gel Immunodiffusion Test
NVSL-SOP-1164 .05 Mycobacterium bovis Gamma Interferon Test Kit for Cattle
Preparation of African Swine Fever Virus (ASFV) Antigen Plates and
NVSL-SOP-1193 .01
Strips for Antibody Detection
Preparation, Performance, and Interpretation of the African and
Classical Swine Fever Real-Time Reverse-Transcription Multiplex
NVSL-SOP-1201 .02
Polymerase Chain Reaction (rRT-PCR) on the Applied Biosystems™
QuantStudio™ 5 Real-Time System
Preparation, Performance, and Interpretation of the African and
Classical Swine Fever Real-Time Reverse-Transcription Multiplex
NVSL-SOP-1203 .02
Polymerase Chain Reaction (rRT-PCR) on the Applied Biosystems™
7500 Real-Time System

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