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Chromatography

The experiment aims to separate the colored components in brown ink using ascending paper chromatography and to calculate their Rf values. The procedure involves applying the ink to a filter paper, using distilled water as a solvent, and measuring the distances traveled by the ink spots and solvent front. The results will include the calculated Rf values for red, yellow, and green inks.

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Saksham Gaming
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55 views2 pages

Chromatography

The experiment aims to separate the colored components in brown ink using ascending paper chromatography and to calculate their Rf values. The procedure involves applying the ink to a filter paper, using distilled water as a solvent, and measuring the distances traveled by the ink spots and solvent front. The results will include the calculated Rf values for red, yellow, and green inks.

Uploaded by

Saksham Gaming
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd

EXPERIMENT NO.

AIM: To Separate the coloured components present in the BROWN ink of


sketch pen by ascending paper chromatography and find their Rf values.

REQUIREMENT: Chromatogram ,brown sketch pen ,distilled water ,boiling


test tube ,scale ,pencil etc.

THEORY: The word chromatography originated from two greek words


'chroma' meaning 'colour' and 'graphine' meaning 'to write'. Chromatography
means colour writing and it was first employed by a Russian scientist Mikhail
Tsvet. This method was first used for the separation of coloured substances in
plants.

In the chromatographic technique, the mixture of substances is applied onto a


phase called the stationary phase. The stationary phase may be solid or liquid. A
moving phase that can be a pure solvent or a mixture of solvents, or a gas is
allowed to move slowly over the stationary phase. This moving phase is called
the mobile phase. When the mobile phase is moved over the mixture on the
stationary phase, the components of the mixture gradually separates from one
another.

PROCEDURE

 Take a Whatman filter paper strip and using a pencil draw a horizontal line
4cm from one end of the paper. Then draw another line lengthwise
(verticallly) from the centre of the paper. Name the point at which the two
lines intersect as P.
 Using a brown sketch pen put a dot at the point P. Let it dry in air.
 Put another dot on the same spot and dry again, so that the spot is rich in the
mixture.
 Pour distilled water into a boiling test tube as the solvent.
 Suspend the filter paper vertically in the boiling test tube containing the
solvent in such a way that the pencil line remains about 2cm above the
solvent level and keep it undisturbed till the components get separated .
 Take the chromatogram out of the boiling test tube and using a pencil mark
the distance that the solvent has risen on the paper. This is called the solvent
front.
 Dry the filter paper and put pencil marks at the centre of the red,yellow and
green ink spots.
 Measure the distance of the three spots from the original line and the
distance of the solvent from the original line.
 Calculate the Rf values of the red ,yellow and blue inks using the formula,

OBSERVATION

Distance travelled by the Distance travelled by the


SI
Components component from the original solvent from the original Rf value
No.
line (cm) line (cm)
1. Red
2. Yellow
3. Green
NOTE: make observation table on plane page after pasting chromatogram

RESULT

 Rf value of red ink =.....................,


 Rf value of Yellow ink =.....................,
 Rf value of Blue ink =.....................,

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